pBAD33

pBAD33

pBAD33载体基本信息

        pBAD33载体复制子是pBR322 ori,f1 ori，质粒大小是5352bp，带有Ampicillin (氨苄青霉素)抗性，可转化进大肠杆菌DH5α，通过在含有Ampicillin (氨苄青霉素)抗性的平板上培养筛选大量复制扩增，培养条件是LB，37℃。
        pBAD33载体的启动子是araC，能在大肠杆菌DE3工程菌（建议用BL21 DE3或Rosetta DE3）中，用IPTG诱导表达出重组蛋白，可选用镍柱纯化。
        pBAD33载体的 5' 测序引物:是pBAD-F: ATGCCATAGCATTTTTATCC， 3' 测序引物是pBAD-R: GATTTAATCTGTATCAGG。

 pBAD33载体质粒图谱和多克隆位点信息

 pBAD33载体序列

ORIGIN
        1 ATCGATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC
       61 TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA
      121 TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA
      181 AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA
      241 GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG
      301 CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG
      361 CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG
      421 TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT
      481 TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC
      541 CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC
      601 GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA
      661 TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA
      721 TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA
      781 ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA
      841 TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC
      901 GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT
      961 TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG AAGAAACCAA TTGTCCATAT
     1021 TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA
     1081 ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT
     1141 AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA
     1201 CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG ATCCTACCTG ACGCTTTTTA
     1261 TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGCGAATT CGAGCTCGGT
     1321 ACCCGGGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC TTGGCTGTTT TGGCGGATGA
     1381 GAGAAGATTT TCAGCCTGAT ACAGATTAAA TCAGAACGCA GAAGCGGTCT GATAAAACAG
     1441 AATTTGCCTG GCGGCAGTAG CGCGGTGGTC CCACCTGACC CCATGCCGAA CTCAGAAGTG
     1501 AAACGCCGTA GCGCCGATGG TAGTGTGGGG TCTCCCCATG CGAGAGTAGG GAACTGCCAG
     1561 GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT
     1621 GTCGGTGAAC GCTCTCCTGA GTAGGACAAA TCCGCCGGGA GCGGATTTGA ACGTTGCGAA
     1681 GCAACGGCCC GGAGGGTGGC GGGCAGGACG CCCGCCATAA ACTGCCAGGC ATCAAATTAA
     1741 GCAGAAGGCC ATCCTGACGG ATGGCCTTTT TGCGTTTCTA CAAACTCTTT TGTTTATTTT
     1801 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT
     1861 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT
     1921 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG
     1981 CTGAAGATCA GTTGGGGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA
     2041 CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT
     2101 CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC
     2161 ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG
     2221 AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT
     2281 AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTACGCGCC
     2341 CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT
     2401 TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC
     2461 CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT
     2521 ACGGCACCTC GACCCCAAAA AACTTGATTT GGGTGATGGT TCACGTAGTG GGCCATCGCC
     2581 CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT
     2641 GTTCCAAACT TGAACAACAC TCAACCCTAT CTCGGGCTAT TCTTTTGATT TATAAGGGAT
     2701 TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA
     2761 TTTTAACAAA ATATTAACGT TTACAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     2821 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     2881 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     2941 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     3001 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC
     3061 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     3121 TCCTGTTACC AGTCAGGCAT TTGAGAAGCA CACGGTCACA CTGCTTCCGG TAGTCAATAA
     3181 ACCGGTAAAC CAGCAATAGA CATAAGCGGC TATTTAACGA CCCTGCCCTG AACCGACGAC
     3241 CGGGTCGAAT TTGCTTTCGA ATTTCTGCCA TTCATCCGCT TATTATCACT TATTCAGGCG
     3301 TAGCACCAGG CGTTTAAGGG CACCAATAAC TGCCTTAAAA AAATTACGCC CCGCCCTGCC
     3361 ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAGA
     3421 CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT
     3481 TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC
     3541 TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA AACCCTTTAG
     3601 GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT
     3661 GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA
     3721 AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA
     3781 TACGGAATTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA
     3841 ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT
     3901 GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT
     3961 GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATTTTAGCT TCCTTAGCTC
     4021 CTGAAAATCT CGATAACTCA AAAAATACGC CCGGTAGTGA TCTTATTTCA TTATGGTGAA
     4081 AGTTGGAACC TCTTACGTGC CGATCAACGT CTCATTTTCG CCAAAAGTTG GCCCAGGGCT
     4141 TCCCGGTATC AACAGGGACA CCAGGATTTA TTTATTCTGC GAAGTGATCT TCCGTCACAG
     4201 GTATTTATTC GGCGCAAAGT GCGTCGGGTG ATGCTGCCAA CTTACTGATT TAGTGTATGA
     4261 TGGTGTTTTT GAGGTGCTCC AGTGGCTTCT GTTTCTATCA GCTGTCCCTC CTGTTCAGCT
     4321 ACTGACGGGG TGGTGCGTAA CGGCAAAAGC ACCGCCGGAC ATCAGCGCTA GCGGAGTGTA
     4381 TACTGGCTTA CTATGTTGGC ACTGATGAGG GTGTCAGTGA AGTGCTTCAT GTGGCAGGAG
     4441 AAAAAAGGCT GCACCGGTGC GTCAGCAGAA TATGTGATAC AGGATATATT CCGCTTCCTC
     4501 GCTCACTGAC TCGCTACGCT CGGTCGTTCG ACTGCGGCGA GCGGAAATGG CTTACGAACG
     4561 GGGCGGAGAT TTCCTGGAAG ATGCCAGGAA GATACTTAAC AGGGAAGTGA GAGGGCCGCG
     4621 GCAAAGCCGT TTTTCCATAG GCTCCGCCCC CCTGACAAGC ATCACGAAAT CTGACGCTCA
     4681 AATCAGTGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGCGGC
     4741 TCCCTCGTGC GCTCTCCTGT TCCTGCCTTT CGGTTTACCG GTGTCATTCC GCTGTTATGG
     4801 CCGCGTTTGT CTCATTCCAC GCCTGACACT CAGTTCCGGG TAGGCAGTTC GCTCCAAGCT
     4861 GGACTGTATG CACGAACCCC CCGTTCAGTC CGACCGCTGC GCCTTATCCG GTAACTATCG
     4921 TCTTGAGTCC AACCCGGAAA GACATGCAAA AGCACCACTG GCAGCAGCCA CTGGTAATTG
     4981 ATTTAGAGGA GTTAGTCTTG AAGTCATGCG CCGGTTAAGG CTAAACTGAA AGGACAAGTT
     5041 TTGGTGACTG CGCTCCTCCA AGCCAGTTAC CTCGGTTCAA AGAGTTGGTA GCTCAGAGAA
     5101 CCTTCGAAAA ACCGCCCTGC AAGGCGGTTT TTTCGTTTTC AGAGCAAGAG ATTACGCGCA
     5161 GACCAAAACG ATCTCAAGAA GATCATCTTA TTAATCAGAT AAAATATTTG CTCATGAGCC
     5221 CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG
     5281 CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC TGCTCATGTT
     5341 TGACAGCTTA TC
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其他大肠杆菌表达载体：