pET-42b(+)

pET-42b(+)

pet42b载体基本信息

pet42b载体质粒图谱和多克隆位点信息

pet42b载体简介

The pET-42 series is designed for cloning and high-level expression of peptide sequencesfused with the 220 aa GST•Tag™ protein. Unique sites are shown on the circle map. Note that thesequence is numbered by the pBR322 convention, so the T7 expression region is reversed on thecircle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymeraseis shown below. The f1 origin is oriented so that infection with helper phage will produce virionscontaining single stranded DNA that corresponds to the coding strand. Therefore, single strandedsequencing should be performed using the T7 terminator primer (cat. no. 69337-3). Vector encodedsequence can be completely removed when cloning into the PshAI site (as shown below) and thencleaving the GST fusion protein with Factor Xa.

pet42b载体序列

ORIGIN

    1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA

   61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT

  121 TGTTTAGCAG CCTAGGTATT AATCAATTAG TGGTGGTGGT GGTGGTGGTG GTGCTCGAGT

  181 GCGGCCGCAA GCTTGTCGAC GGAGCTCGCC TGCAGGCGCG CCAAGGCCTG TACAGAATTC

  241 GGATCCCCGA TATCCATGGA CCCGCGTCCC TCAATACCGG AGCCACCACC GGTACCCAGA

  301 TCTGGGCTGT CCATGTGCTG GCGTTCGAAT TTAGCAGCAG CGGTTTCTTT CATACCAATT

  361 GCAGTACTAC CGCGTGGCAC CAGACCCGCG GAGTGATGGT GATGGTGATG ACCAGAACCA

  421 CTAGTTGAAC CATCCGATTT TGGAGGATGG TCGCCACCAC CAAACGTGGC TTGCCAGCCC

  481 TGCAAAGGCC ATGCTATATA CTTGCTGGAT TTCAAGTACT TATCAATTTG TGGGATAGCT

  541 TCAATACGTT TTTTAAAACA AACTAATTTT GGGAACGCAT CCAGGCACAT TGGGTCCATG

  601 TATAAAACAA CATCAAGAGC GTCATACAAC ATGAAGTCAG GATGGGTTAC ATGATCACCA

  661 TTTAAATATG TTTTATGACA TAAACGATCT TCGAACATTT TCAGCATTTC AGGTAGCTTG

  721 CTAAGAAAAT CAACTTTGAG AGTTTCAAAG TCTTTACTAT ATGCAATTCT CGAAACACCG

  781 TATCTAATAT CCAAAACCGC TCCTTCAAGC ATTGAAATCT CTGCACGCTC TTTTGGACAA

  841 CCACCCAACA TGTTGTGCTT GTCAGCTATA TAACGTATGA TGGCCATAGA CTGTGTTAAT

  901 TTAACATCAC CATCAATATA ATAAGGAAGA TTGGGAAACT CCAAACCCAA TTCAAACTTT

  961 TTGTTTCGCC ATTTATCACC TTCATCGCGC TCATACAAAT GCTCTTCATA TTTTTCTTCA

 1021 AGATATTCCA AAAGAAGTCG AGTGGGTTGC ACAAGGCCCT TAATTTTCCA ATAACCTAGT

 1081 ATAGGGGACA TATGTATATC TCCTTCTTAA AGTTAAACAA AATTATTTCT AGAGGGGAAT

 1141 TGTTATCCGC TCACAATTCC CCTATAGTGA GTCGTATTAA TTTCGCGGGA TCGAGATCGA

 1201 TCTCGATCCT CTACGCCGGA CGCATCGTGG CCGGCATCAC CGGCGCCACA GGTGCGGTTG

 1261 CTGGCGCCTA TATCGCCGAC ATCACCGATG GGGAAGATCG GGCTCGCCAC TTCGGGCTCA

 1321 TGAGCGCTTG TTTCGGCGTG GGTATGGTGG CAGGCCCCGT GGCCGGGGGA CTGTTGGGCG

 1381 CCATCTCCTT GCATGCACCA TTCCTTGCGG CGGCGGTGCT CAACGGCCTC AACCTACTAC

 1441 TGGGCTGCTT CCTAATGCAG GAGTCGCATA AGGGAGAGCG TCGAGATCCC GGACACCATC

 1501 GAATGGCGCA AAACCTTTCG CGGTATGGCA TGATAGCGCC CGGAAGAGAG TCAATTCAGG

 1561 GTGGTGAATG TGAAACCAGT AACGTTATAC GATGTCGCAG AGTATGCCGG TGTCTCTTAT

 1621 CAGACCGTTT CCCGCGTGGT GAACCAGGCC AGCCACGTTT CTGCGAAAAC GCGGGAAAAA

 1681 GTGGAAGCGG CGATGGCGGA GCTGAATTAC ATTCCCAACC GCGTGGCACA ACAACTGGCG

 1741 GGCAAACAGT CGTTGCTGAT TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG

 1801 CAAATTGTCG CGGCGATTAA ATCTCGCGCC GATCAACTGG GTGCCAGCGT GGTGGTGTCG

 1861 ATGGTAGAAC GAAGCGGCGT CGAAGCCTGT AAAGCGGCGG TGCACAATCT TCTCGCGCAA

 1921 CGCGTCAGTG GGCTGATCAT TAACTATCCG CTGGATGACC AGGATGCCAT TGCTGTGGAA

 1981 GCTGCCTGCA CTAATGTTCC GGCGTTATTT CTTGATGTCT CTGACCAGAC ACCCATCAAC

 2041 AGTATTATTT TCTCCCATGA AGACGGTACG CGACTGGGCG TGGAGCATCT GGTCGCATTG

 2101 GGTCACCAGC AAATCGCGCT GTTAGCGGGC CCATTAAGTT CTGTCTCGGC GCGTCTGCGT

 2161 CTGGCTGGCT GGCATAAATA TCTCACTCGC AATCAAATTC AGCCGATAGC GGAACGGGAA

 2221 GGCGACTGGA GTGCCATGTC CGGTTTTCAA CAAACCATGC AAATGCTGAA TGAGGGCATC

 2281 GTTCCCACTG CGATGCTGGT TGCCAACGAT CAGATGGCGC TGGGCGCAAT GCGCGCCATT

 2341 ACCGAGTCCG GGCTGCGCGT TGGTGCGGAC ATCTCGGTAG TGGGATACGA CGATACCGAA

 2401 GACAGCTCAT GTTATATCCC GCCGTTAACC ACCATCAAAC AGGATTTTCG CCTGCTGGGG

 2461 CAAACCAGCG TGGACCGCTT GCTGCAACTC TCTCAGGGCC AGGCGGTGAA GGGCAATCAG

 2521 CTGTTGCCCG TCTCACTGGT GAAAAGAAAA ACCACCCTGG CGCCCAATAC GCAAACCGCC

 2581 TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA

 2641 AGCGGGCAGT GAGCGCAACG CAATTAATGT AAGTTAGCTC ACTCATTAGG CACCGGGATC

 2701 TCGACCGATG CCCTTGAGAG CCTTCAACCC AGTCAGCTCC TTCCGGTGGG CGCGGGGCAT

 2761 GACTAGCATG ATCGTGCTCC TGTCGTTGAG GACCCGGCTA GGCTGGCGGG GTTGCCTTAC

 2821 TGGTTAGCAG AATGAATCAC CGATACGCGA GCGAACGTGA AGCGACTGCT GCTGCAAAAC

 2881 GTCTGCGACC TGAGCAACAA CATGAATGGT CTTCGGTTTC CGTGTTTCGT AAAGTCTGGA

 2941 AACGCGGAAG TCAGCGCCCT GCACCATTAT GTTCCGGATC TGCATCGCAG GATGCTGCTG

 3001 GCTACCCTGT GGAACACCTA CATCTGTATT AACGAAGCGC TGGCATTGAC CCTGAGTGAT

 3061 TTTTCTCTGG TCCCGCCGCA TCCATACCGC CAGTTGTTTA CCCTCACAAC GTTCCAGTAA

 3121 CCGGGCATGT TCATCATCAG TAACCCGTAT CGTGAGCATC CTCTCTCGTT TCATCGGTAT

 3181 CATTACCCCC ATGAACAGAA ATCCCCCTTA CACGGAGGCA TCAGTGACCA AACAGGAAAA

 3241 AACCGCCCTT AACATGGCCC GCTTTATCAG AAGCCAGACA TTAACGCTTC TGGAGAAACT

 3301 CAACGAGCTG GACGCGGATG AACAGGCAGA CATCTGTGAA TCGCTTCACG ACCACGCTGA

 3361 TGAGCTTTAC CGCAGCTGCC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT

 3421 GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG

 3481 TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG

 3541 CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG

 3601 CACCATATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC

 3661 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG

 3721 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA

 3781 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG

 3841 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA

 3901 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG

 3961 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG

 4021 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC

 4081 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG

 4141 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA

 4201 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT

 4261 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG

 4321 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG

 4381 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC

 4441 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT

 4501 TGGTCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA ATACAAGGGG TGTTATGAGC

 4561 CATATTCAAC GGGAAACGTC TTGCTCTAGG CCGCGATTAA ATTCCAACAT GGATGCTGAT

 4621 TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGA

 4681 TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC

 4741 AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT GCCTCTTCCG

 4801 ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC TGCGATCCCC

 4861 GGGAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA TATTGTTGAT

 4921 GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC

 4981 AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT

 5041 GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG GAAAGAAATG

 5101 CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT CTCACTTGAT

 5161 AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG AGTCGGAATC

 5221 GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA

 5281 TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG

 5341 TTTCATTTGA TGCTCGATGA GTTTTTCTAA GAATTAATTC ATGAGCGGAT ACATATTTGA

 5401 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC

 5461 TGAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC

 5521 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA

 5581 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC

 5641 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC

 5701 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG

 5761 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA

 5821 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC

 5881 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCA

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其他大肠杆菌表达载体：