北京华越洋生物
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3

pKD4

pKD4

pKD4

 

编号

名称

北京华越洋VECT-510

pKD4

 

pKD4载体基本信息

载体名称:

pKD4

质粒类型:

细菌表达;基因敲除系统

高拷贝/低拷贝:

--

启动子:

--

克隆方法:

多克隆位点,限制性内切酶

载体大小:

3267 bp

5' 测序引物及序列:

Amp-R: ATAATACCGCGCCACATAGC

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

氨苄青霉素(Ampicillin

筛选标记:

新霉素(Neomycin

备注:

--

稳定性:

--

组成型:

--

病毒/非病毒:

--

 

pKD4载体质粒图谱和多克隆位点信息


pKD4载体简介

pKD4载体是RED系统敲除载体,复制子是R6K y ori,质粒大小是3267bp,带有Ampicillin(Amp)KanamycinKan)抗性,可转化进大肠杆菌DH5α,通过抗生素抗性筛选大量复制扩增,培养条件是LB30℃。

 

pKD4载体序列

ORIGIN

    1 agattgcagc attacacgtc ttgagcgatt gtgtaggctg gagctgcttc gaagttccta

   61 tactttctag agaataggaa cttcggaata ggaacttcaa gatcccctca cgctgccgca

  121 agcactcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc agtccgcaga

  181 aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa

  241 gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg

  301 ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga

  361 agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat

  421 caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc

  481 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga

  541 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt

  601 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat

  661 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg

  721 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg

  781 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc

  841 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga

  901 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag

  961 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc

 1021 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg

 1081 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata

 1141 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg

 1201 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac

 1261 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc

 1321 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat

 1381 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccagct tcaaaagcgc

 1441 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaacta aggaggatat

 1501 tcatatggac catggctaat tcccatgtca gccgttaagt gttcctgtgt cactgaaaat

 1561 tgctttgaga ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac

 1621 cgttaaacct taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa

 1681 accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct

 1741 tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg

 1801 agggtttagt tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga

 1861 aacatgagag cttagtacgt actatcaaca ggttgaactg cggatcttgc ggccgcaaaa

 1921 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt

 1981 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag

 2041 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca

 2101 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc

 2161 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt

 2221 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg

 2281 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca

 2341 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg

 2401 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca

 2461 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg

 2521 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct

 2581 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca

 2641 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca

 2701 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg

 2761 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac

 2821 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt

 2881 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc

 2941 cgcgcacatt tccccgaaaa gtgccacctg catcgatggc cccccgatgg tagtgtgggg

 3001 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa

 3061 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa

 3121 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg

 3181 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt

 3241 tgcgtggcca gtgccaagct tgcatgc

//

 

其他大肠杆菌表达载体:

pKD13

PinPoint Xa-1

pKD4

pTf16

pTWIN2

pTYB11

pKJE7

pET-17b

pTrcHis2 A

pBad/gIII B

pGEX-6P-2

pG-KJE8

pET-46 EK/LIC

pMal-p2G

pGEX-2TK

pGEX-4T-3

pET-49b(+)

pEZZ18

pMal-c2X

pET300/NT-DEST

pBAD-TOPO

pMal-p2X

pET-33b(+)

pRSET-CFP

pET-23(+)

pMal-c4X

pET-24c(+)

pTrcHis2 B

pET-11b(+)

pET-41a(+)

pET-24(+)

pET-44c(+)

pQE-82L

pGEX-4T-1

pMal-p4X

pET-43.1c(+)

pQE-9

pALEX a,b,c

pBad/gIII C

pET-27b(+)

pQE-70

pKD20

pET102/D-TOPO

pET-21a(+)

pET-16b

pRSET B

pACYCDuet-1

pET-20b(+)

pET-21d(+)

pCOLADuet-1

pCYB1

pET-14b

pRSET-EmGFP

pET-42b(+)

pGEX-5X-2

pET-26b(+)

pET301/CT-DEST

pGEX-6P-1

pET-28c(+)

pET-50b(+)

pGro7

pRSET A

pET-32a(+)

pMal-c2G

pET-11a(+)

pRSET C

ptdTomato

pQE-30

pET-12b(+)

pET-42c(+)

pET-43.1b(+)

pET-41c(+)

pET-23b(+)

pET-39b(+)

pBV220

pET-41c(+)

pBad/His C

pET-32b(+)

pCold IV

pETDuet-1

pBAD/Thio-TOPO

pET-29c(+)

pE-SUMO

pTYB12

pCold-ProS2

pET-24b(+)

pACYC184

pEGM-7ZF(+)

pCold III

pBAD33

pBad/Myc-His A

pSP73

pCold-GST

pAmCyan

PinPoint Xa-2

pTXB1

pET-52b(+)

pColdS-SUMO

pTWIN1

pBad/gIII A

pET-23d(+)

pCold II

pET-24a(+)

pkk223-3

pET-29a(+)

pBAD102/D-TOPO

pET-28a(+)

pBAD18

pET-30 EK/LIC

pBad/Myc-His C

pProEX HTc

pET-44 EK/LIC

pET-32 Xa/LIC

pBad/His B

pMal-p2E

pTrcHis B

pET-40b(+)

pET-23a(+)

pQE-60

pGEX-3X

pET-45b(+)

pET-12c(+)

pQE-32

pTrc99a

pMal-c2E

pET-11c(+)

pBad24

pACYC177

pGEX-5X-1

pQE-80L

pET-12a(+)

pMXB10

pGEX-6P-3

pQE-31

pET-23c(+)

pGEX-KG

pTrcHis A

pET-51b(+)

pBad/His A

pTrcHis C

pGEX-2T

pET-47b(+)

pBad/Myc-His B

pET-3a(+)

pEcoli-6xHN-GFPuv

pET-22b(+)

pBAD202/D-TOPO

pGEX-5X-3

pBR322

pET-19b

pCold I

pET-32 EK/LIC

pET-28b(+)

pET-21b(+)

pCold TF

pET-30 Xa/LIC

pGEX-4T-2

pET-25b(+)

pQE-81L

pET-29b(+)

pET-31b(+)

pET-42a(+)

pQE-16

pGEM-T

pET-43.1 EK/LIC

pET-44a(+)

pQE-40

pBlueScript SK(+)

pMAL-c5x

pTrcHis2 C

pET-48b(+)

pET-SUMO

pkk232-8

pET302/NT-His

pET-21b(+)

pCDFDuet-1

pET-5a(+)

pGFPuv

pET-15b

pBluescript II SK(+)

pET-5b(+)

pBad43

pET-21c(+)

pProEX HTb

pSP64

pG-Tf2

pET-24d(+)

pKD46

PinPoint Xa-3

pSE380

pET-30a(+)

pBluescript II KS(-)

pMAL-p5e

pMAL-p5x

pET-43.1a(+)

pEGM-11ZF(+)

pTYB2

pET-30b(+)

pET-37b(+)

pDsRed-Express2

pKD3

pProEX HTa

pET303/CT-His

pBV221

pSUMO

pTYB1

pRSET-BFP

pET-11d(+)

pET-3b(+)

pET-41 EK/LIC

pET-32c(+)

pET-44b(+)

pRSFDuet-1

pET-30c(+)

pET-His

pET-41b(+)