pKD4
pKD4
编号 | 名称 |
北京华越洋VECT-510 | pKD4 |
pKD4载体基本信息
载体名称: | pKD4 |
质粒类型: | 细菌表达;基因敲除系统 |
高拷贝/低拷贝: | -- |
启动子: | -- |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 3267 bp |
5' 测序引物及序列: | Amp-R: ATAATACCGCGCCACATAGC |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素(Ampicillin) |
筛选标记: | 新霉素(Neomycin) |
备注: | -- |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | -- |
pKD4载体质粒图谱和多克隆位点信息
pKD4载体简介
pKD4载体是RED系统敲除载体,复制子是R6K y ori,质粒大小是3267bp,带有Ampicillin(Amp)和Kanamycin(Kan)抗性,可转化进大肠杆菌DH5α,通过抗生素抗性筛选大量复制扩增,培养条件是LB,30℃。
pKD4载体序列
ORIGIN
1 agattgcagc attacacgtc ttgagcgatt gtgtaggctg gagctgcttc gaagttccta
61 tactttctag agaataggaa cttcggaata ggaacttcaa gatcccctca cgctgccgca
121 agcactcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc agtccgcaga
181 aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa
241 gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg
301 ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga
361 agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat
421 caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
481 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
541 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
601 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
661 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
721 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
781 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
841 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
901 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
961 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
1021 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
1081 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
1141 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
1201 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
1261 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
1321 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
1381 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccagct tcaaaagcgc
1441 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaacta aggaggatat
1501 tcatatggac catggctaat tcccatgtca gccgttaagt gttcctgtgt cactgaaaat
1561 tgctttgaga ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac
1621 cgttaaacct taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa
1681 accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct
1741 tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg
1801 agggtttagt tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga
1861 aacatgagag cttagtacgt actatcaaca ggttgaactg cggatcttgc ggccgcaaaa
1921 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
1981 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
2041 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
2101 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
2161 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
2221 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
2281 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
2341 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
2401 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
2461 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
2521 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
2581 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
2641 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
2701 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
2761 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
2821 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
2881 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
2941 cgcgcacatt tccccgaaaa gtgccacctg catcgatggc cccccgatgg tagtgtgggg
3001 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
3061 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
3121 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg
3181 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt
3241 tgcgtggcca gtgccaagct tgcatgc
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