pET-41 EK/LIC

pET41 EK/LIC载体基本信息

pET41 EK/LIC载体质粒图谱和多克隆位点信息

pET41 EK/LIC载体简介

The pET-41 Ek/LIC vector is prepared for rapid, directional cloning of PCR-amplified DNA for high-level expression of polypeptides fused with N-terminal GST•Tag™, His•Tag® and S•Tag™ sequences. Using specifically designed primers for amplification and the pET-41 Ek/LIC Vector Kit (Cat. No. 71071-3), inserts can be efficiently cloned without the need for restriction digestion or ligation. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3). Vector encoded sequence can be completely removed when cloning into the Ek/LIC site (as shown below left) by cleaving the fusion protein with enterokinase.

pET41 EK/LIC载体序列

ORIGIN

    1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa

   61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt

  121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt

  181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc

  241 ggatccccga tatccatggt tgaggagaag cccgggctct tgtcgtcgtc atcaccagaa

  301 ccaccaccgg tacccagatc tgggctgtcc atgtgctggc gttcgaattt agcagcagcg

  361 gtttctttca taccaattgc agtactaccg cgtggcacca gacccgcgga gtgatggtga

  421 tggtgatgac cagaaccact agttgaacca tccgattttg gaggatggtc gccaccacca

  481 aacgtggctt gccagccctg caaaggccat gctatatact tgctggattt caagtactta

  541 tcaatttgtg ggatagcttc aatacgtttt ttaaaacaaa ctaattttgg gaacgcatcc

  601 aggcacattg ggtccatgta taaaacaaca tcaagagcgt catacaacat gaagtcagga

  661 tgggttacat gatcaccatt taaatatgtt ttatgacata aacgatcttc gaacattttc

  721 agcatttcag gtagcttgct aagaaaatca actttgagag tttcaaagtc tttactatat

  781 gcaattctcg aaacaccgta tctaatatcc aaaaccgctc cttcaagcat tgaaatctct

  841 gcacgctctt ttggacaacc acccaacatg ttgtgcttgt cagctatata acgtatgatg

  901 gccatagact gtgttaattt aacatcacca tcaatataat aaggaagatt gggaaactcc

  961 aaacccaatt caaacttttt gtttcgccat ttatcacctt catcgcgctc atacaaatgc

 1021 tcttcatatt tttcttcaag atattccaaa agaagtcgag tgggttgcac aaggccctta

 1081 attttccaat aacctagtat aggggacata tgtatatctc cttcttaaag ttaaacaaaa

 1141 ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt

 1201 tcgcgggatc gagatcgatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg

 1261 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg

 1321 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg

 1381 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca

 1441 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc

 1501 gagatcccgg acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg

 1561 gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag

 1621 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct

 1681 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc

 1741 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg

 1801 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt

 1861 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg

 1921 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag

 1981 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct

 2041 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg

 2101 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct

 2161 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag

 2221 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa

 2281 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg

 2341 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggacat ctcggtagtg

 2401 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag

 2461 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag

 2521 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg

 2581 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga

 2641 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtaa gttagctcac

 2701 tcattaggca ccgggatctc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt

 2761 ccggtgggcg cggggcatga ctagcatgat cgtgctcctg tcgttgagga cccggctagg

 2821 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag

 2881 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg

 2941 tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg

 3001 catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg

 3061 gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc

 3121 ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct

 3181 ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc

 3241 agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt

 3301 aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc

 3361 gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg

 3421 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc

 3481 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc

 3541 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag

 3601 cagattgtac tgagagtgca ccatatatgc ggtgtgaaat accgcacaga tgcgtaagga

 3661 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg

 3721 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat

 3781 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta

 3841 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa

 3901 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc

 3961 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt

 4021 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca

 4081 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg

 4141 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat

 4201 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta

 4261 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct

 4321 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac

 4381 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa

 4441 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa

 4501 actcacgtta agggattttg gtcatgaaca ataaaactgt ctgcttacat aaacagtaat

 4561 acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctctaggcc gcgattaaat

 4621 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca

 4681 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat

 4741 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg

 4801 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta

 4861 ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca

 4921 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt

 4981 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg

 5041 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa

 5101 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat

 5161 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat

 5221 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc

 5281 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct

 5341 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaaga attaattcat

 5401 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt

 5461 tccccgaaaa gtgccacctg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa

 5521 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa

 5581 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact

 5641 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc

 5701 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa

 5761 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc

 5821 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt

 5881 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca

 5941 ttcgcca

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