pGEX-2TK

pGEX2TK载体基本信息

pGEX2TK载体质粒图谱和多克隆位点信息

pGEX2TK载体简介

There are a range of Gluthatione Sepharose™ prepacked column and bulk media products available to purify GST Fusion proteins. For manual purification of sample volumes up to 600 µl use GST SpinTrap™ microspin columns or GST MultiTrap™ 4B 96-well plates. For sample volumes between 600 µl and 10 ml use GST GraviTrap™ gravity flow column. Where sample volumes are above 10 ml, use LabMate™ reservoir together with GST GraviTrap. All formats described can be used for preparation of samples in parallel. In addition GST HiTrap™ 1 and 5 ml columns and GST HiPrep™ FF 16/10 column are available for purification in a chromatography system such as the ÄKTA™ design system. Alternatively, Gluthatione Sepharose bulk media are available from 10 ml up to 500 ml. A GST Bulk Kit is also available combining 10 ml Gluthatione Sepharose 4B bulk medium and 5 empty columns with required buffers.

pGEX系列载体：

         pGEX-1λT EcoRI/BAP

         pGEX-2T

         pGEX-2TK

         pGEX-20T

         pGEX-3X

         pGEX-4T-1

         pGEX-4T-2

         pGEX-4T-3

         pGEX-5X-1

         pGEX-5X-2

         pGEX-5X-3

         pGEX-6P-1

         pGEX-6P-2

         pGEX-6P-3

         pGEX-KG

pGEX2TK载体序列

ORIGIN

    1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg

   61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt

  121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc

  181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca

  241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc

  301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc

  361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc

  421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata

  481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc

  541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact

  601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag

  661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt

  721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa

  781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat

  841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc

  901 atcctccaaa atcggatctg gttccgcgtg gatctcgtcg tgcatctgtt ggatccccgg

  961 gaattcatcg tgactgactg acgatctgcc tcgcgcgttt cggtgatgac ggtgaaaacc

 1021 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca

 1081 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc

 1141 agtcacgtag cgatagcgga gtgtataatt cttgaagacg aaagggcctc gtgatacgcc

 1201 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc

 1261 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc

 1321 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga

 1381 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt

 1441 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag

 1501 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag

 1561 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg

 1621 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg

 1681 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca

 1741 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag

 1801 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc

 1861 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg

 1921 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc

 1981 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg

 2041 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg

 2101 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga

 2161 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac

 2221 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa

 2281 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca

 2341 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag

 2401 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac

 2461 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa

 2521 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc

 2581 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag

 2641 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac

 2701 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc

 2761 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc

 2821 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca

 2881 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc

 2941 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg

 3001 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct

 3061 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata

 3121 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc

 3181 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc ataaattccg

 3241 acaccatcga atggtgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc

 3301 aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg

 3361 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc

 3421 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac

 3481 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg

 3541 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg

 3601 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc

 3661 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg

 3721 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac

 3781 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg

 3841 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc

 3901 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg

 3961 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg

 4021 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc

 4081 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg

 4141 ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc

 4201 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg

 4261 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc

 4321 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc

 4381 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca

 4441 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa

 4501 caatttcaca caggaaacag ctatgaccat gattacggat tcactggccg tcgttttaca

 4561 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc

 4621 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg

 4681 cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag

 4741 ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat

 4801 gcacggttac gatgcgccca tctacaccaa cgtaacctat cccattacgg tcaatccgcc

 4861 gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag

 4921 ctggctacag gaaggccaga cgcgaattat ttttgatggc gttggaatt

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