pKD13

pKD13载体基本信息

pKD13载体质粒图谱和多克隆位点信息

pKD13载体简介

pKD13载体是RED系统敲除载体载体，复制子是R6K Y ori，质粒大小是3434bp，带有Ampicillin (氨苄青霉素), Kanamycin(卡那霉素), Chloramphenicol(氯霉素)抗性，可转化进大肠杆菌DH5α，通过抗生素抗性筛选大量复制扩增，培养条件是LB，30℃。

pKD13载体序列

ORIGIN     

        1 agattgcagc attacacgtc ttgagcgatt gtgtaggctg gagctgcttc gaagttccta

       61 tactttctag agaataggaa cttcggaata ggaacttcaa gatcccctta ttagaagaac

      121 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc

      181 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac

      241 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag

      301 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc

      361 tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga

      421 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc

      481 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc

      541 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg

      601 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg

      661 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg

      721 tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc

      781 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca

      841 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca

      901 atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag

      961 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag

     1021 ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat

     1081 cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc

     1141 cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc

     1201 tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca gcgtgagctt

     1261 caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcgaac tgcaggtcga

     1321 cggatccccg gaattaattc tcatgtttga cagcttatca ctgatcagtg aattaatggc

     1381 gatgacgcat cctcacgata atatccgggt aggcgcaatc actttcgtct ctactccgtt

     1441 acaaagcgag gctgggtatt tcccggcctt tctgttatcc gaaatccact gaaagcacag

     1501 cggctggctg aggagataaa taataaacga ggggctgtat gcacaaagca tcttctgttg

     1561 agttaagaac gagtatcgag atggcacata gccttgctca aattggaatc aggtttgtgc

     1621 caataccagt agaaacagac gaagaagcta gctttgcact ggattgcgag gctttgccat

     1681 ggctaattcc catgtcagcc gttaagtgtt cctgtgtcac tgaaaattgc tttgagaggc

     1741 tctaagggct tctcagtgcg ttacatccct ggcttgttgt ccacaaccgt taaaccttaa

     1801 aagctttaaa agccttatat attctttttt ttcttataaa acttaaaacc ttagaggcta

     1861 tttaagttgc tgatttatat taattttatt gttcaaacat gagagcttag tacgtgaaac

     1921 atgagagctt agtacgttag ccatgagagc ttagtacgtt agccatgagg gtttagttcg

     1981 ttaaacatga gagcttagta cgttaaacat gagagcttag tacgtgaaac atgagagctt

     2041 agtacgtact atcaacaggt tgaactgcgg atcttgcggc cgcaaaaatt aaaaatgaag

     2101 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat

     2161 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc

     2221 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat

     2281 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag

     2341 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg

     2401 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc

     2461 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca

     2521 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg

     2581 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc

     2641 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta

     2701 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc

     2761 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg

     2821 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc

     2881 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc

     2941 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat

     3001 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag

     3061 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc

     3121 ccgaaaagtg ccacctgcat cgatggcccc ccgatggtag tgtggggtct ccccatgcga

     3181 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt

     3241 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg

     3301 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact

     3361 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtggccagtg

     3421 ccaagcttgc atgc

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