pRSFDuet-1

pRSFDuet1载体基本信息

pRSFDuet1载体质粒图谱和多克隆位点信息

pRSFDuet1载体简介

pRSFDuet-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). The vector also carries the RSF1030-derived RSF replicon, lacI gene and kanamycin resistance gene (KnR). This vector can be used in combination with pACYCDuet™-1 , pCDFDuet™-1, and pETDuet™-1 in an appropriate host strain for the coexpression of 4 to 8 target proteins. ORFs inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer. Unique sites are shown on the circle map.

pRSFDuet1载体序列

ORIGIN

    1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag

   61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag

  121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag

  181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt

  241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata

  301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt

  361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg

  421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg

  481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg

  541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt

  601 aacgaccctg ccctgaaccg acgacaagct gacgaccggg tctccgcaag tggcactttt

  661 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat

  721 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt

  781 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa

  841 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg

  901 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa

  961 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca

 1021 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc

 1081 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cggtcgctgt taaaaggaca

 1141 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt

 1201 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt

 1261 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat

 1321 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc

 1381 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt

 1441 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat

 1501 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcatactctt

 1561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt

 1621 tgaatgtatt tagaaaaata aacaaatagg catgcagcgc tcttccgctt cctcgctcac

 1681 tgactcgcta cgctcggtcg ttcgactgcg gcgagcggtg tcagctcact caaaagcggt

 1741 aatacggtta tccacagaat caggggataa agccggaaag aacatgtgag caaaaagcaa

 1801 agcaccggaa gaagccaacg ccgcaggcgt ttttccatag gctccgcccc cctgacgagc

 1861 atcacaaaaa tcgacgctca agccagaggt ggcgaaaccc gacaggacta taaagatacc

 1921 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg

 1981 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgtt

 2041 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg

 2101 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac

 2161 acgacttatc gccactggca gcagccattg gtaactgatt tagaggactt tgtcttgaag

 2221 ttatgcacct gttaaggcta aactgaaaga acagattttg gtgagtgcgg tcctccaacc

 2281 cacttacctt ggttcaaaga gttggtagct cagcgaacct tgagaaaacc accgttggta

 2341 gcggtggttt ttctttattt atgagatgat gaatcaatcg gtctatcaag tcaacgaaca

 2401 gctattccgt tactctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc

 2461 agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg

 2521 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga

 2581 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt

 2641 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc

 2701 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg

 2761 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt

 2821 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact

 2881 accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc

 2941 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc

 3001 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga

 3061 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa

 3121 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg

 3181 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag

 3241 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg

 3301 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc

 3361 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc

 3421 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga

 3481 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg

 3541 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa

 3601 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct

 3661 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg

 3721 cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg

 3781 ctctccctta tgcgactcct gcattaggaa attaatacga ctcactata

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