pET-43.1 EK/LIC

pET-43.1 EK/LIC

pET43.1 EK/LIC载体基本信息

pET43.1 EK/LIC载体质粒图谱和多克隆位点信息

pET43.1 EK/LIC载体简介

The pET-43.1 Ek/LIC vector is prepared for rapid, directional cloning of PCR-amplified DNA for high-level expression of polypeptides fused with N-terminal Nus•Tag™, His•Tag® and S•Tag™ sequences. Using specifically designed primers for amplification and the pET-43.1 Ek/LIC Vector Kit (Cat. No. 71072-3), inserts can be efficiently cloned without the need for restriction digestion or ligation. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the COLIDOWN primer (Cat. No. 70845-3). Vector encoded sequence can be completely removed when cloning into the Ek/LIC site (as shown below left) by cleaving the fusion protein with enterokinase.

pET43.1 EK/LIC载体序列

ORIGIN

    1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA

   61 GGGGTTATGC TAGTTATTGC TCAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA

  121 AAAGAGTTTG TAGAAACGCA AAAAGGCCAT CCGTCAGGAT GGCCTTCTGC TTAATTTGAT

  181 GCCTGGCAGT TTATGGCGGG CGTCCTGCCC GCCACCCTCC GGGCCGTTGC TTCGCAACGT

  241 TCAAATCCGC TCCCGGCGGA TTTGTCCTAC TCAGGAGAGC GTTCACCGAC AAACAACAGA

  301 TAAAACGAAA GGCCCAGTCT TTCGACTGAG CCTTTCGTTT TATTTGATGC CTGGCAGTTC

  361 CCTACTCTCG CATGGGGAGA CCCCACACTA CCATCGGCGC TACGGCGTTT CACTTCTGAG

  421 TTCGGCATGG GGTCAGGTGG GACCACCGCG CTACTGCCGC CAGGCAAATT CTGTTTTATC

  481 AGCCTAGGAA CGCCCAACTT AATTAACATT AGTGGTGGTG GTGGTGGTGC TCGAGATCCT

  541 CTGGGTCTTC AGGAGCGAGT TCTGGCTGGC TTGCACGTGT ATACAGCTGT GCGGCCGCAA

  601 GCTTCGAACG CGTATCGATG GTACCGTCGA CGTCCTGCAG GCGCGCCTGT ACAGAATTCG

  661 GATCCACGAG CTCAGATATC GTTGAGGAGA AGCCCGGACT CTTGTCGTCG TCATCAATCG

  721 TACCAGAACC CGCGCTGCCC CGGGGGACCA GACCAGTTGG CGGTGGCGAG TCCATGTGCT

  781 GGCGTTCAAA TTTCGCAGCA GCGGTTTCTT TACCCGCGGA GTGATGGTGA TGGTGATGAC

  841 CAGAACCACT AGTCGCTTCG TCACCGAACC AGCAAATATT ACGGGCAGCC ATAATCAGTG

  901 CTCCGGCTTT TTCGTCGGTC AACCCTTCGA TATCAGCCAG ATCATCAATG CCCTGTTCGG

  961 CGAGATCTTC CAGCGTACAA ACGCCACGGG CGGCCAGTTT GAATGCCAAA TCACGATCTA

 1021 CCCCTTCAAG GTTCAGCAGA TCGTCAGCCG GTTTGTTATC ACCGAGGCTT TCTTCCTGGG

 1081 CCTGTGCAAT GGTGGCCAGT GCATTTTTAG CACGCTCGCG CAGTGCTTCA ACGGTCGGCT

 1141 CATCAAGGCC TTCGATTTCC AACAGCTCTT TCATCGGCAC ATAGGCCAAT TCTTCCAGCG

 1201 TCGAGAAGCC TTCTTCTACC AGAACAGTCG CGAAGTCTTC GTCGATGTCG AGATATTTGG

 1261 TGAAGGTGTC GATCGCTGCG TGCGCTTCCG CCTGATGCTT AGCTTGCAGG TCGTCAACGG

 1321 TCATCACGTT GAGTTCCCAA CCGCTCAGTT GCGAAGCCAG ACGCACGTTC TGACCGTTAC

 1381 GGCCAATCGC CTGCGCCAGA TTACCGGCTT CAACGGCGAT GTCCATGGTG TGTTTATCTT

 1441 CATCCACCAC GATAGAAGCA ACGTCTGCCG GTGCCATTGC GTTAATCACG AACTGCGCCG

 1501 GGTTATCATC CCACAGGACG ATATCGATAC GCTCGCCACC CAGTTCAGTA GACACCGCCT

 1561 GAACACGCGC GCCACGCATA CCTACGCAAG CACCTACCGG ATCGATACGT TTATCGTTGG

 1621 TTTTCACCGC GATTTTCGCA CGAGAACCCG GATCGCGAGC CGCTGCTTTA ATTTCAATCA

 1681 CTTCTTCGCC GATTTCTGGC ACTTCAATAC GGAACAGTTC GATCAGCATT TCCGGCTTGG

 1741 AACGAGTGAC GAACAGTTGC GCGCCACGCG CTTCCGGGCG AACGGAATAG AGCACGCCAC

 1801 GAACGCGGTC GCCAGGGCGG AAGTTTTCAC GCGGCAGCAT ATCTTCGCGC AGGATCACGG

 1861 CTTCAGCGTT GTTGCCCAGA TCCAGAGAGA TGTTGTCGCG GTTTACTTTT TTCACCACGC

 1921 CGGTGATGAT TTCACCTTCG TGTTCACGGA ACTGATCAAC CACCATCGCA CGTTCGGCTT

 1981 CACGCACTTT CTGCACGATA ACCTGTTTTG CCGTCTGGGT AGTGATACGG TCAAAGGTAA

 2041 CAGACTCAAT CTGATCTTCA ACGTAATCGC CCAGGTTCAG GCTTTCATCT TCATAACGTG

 2101 CGGCTTCAAG GGTGATTTCC TTGGTCGGCT GGGTGACTTC ATCAACAACT AACCAGCGAC

 2161 GGAAAGTGTC AAAATCACCG CTTTTGCGAT CGATCTGTAC GCGGACGTCG ATCTCTTGTT

 2221 CATATTTTTT CTTTGTTGCT GTCGCCAGCG CGCTTTCCAA TGCTTCGAAA ATCTTCTCGC

 2281 GAGGTAGCGC CTTTTCATTG GATACGGCTT CAACTACAGC CAAAATTTCT TTGTTCATAT

 2341 GTATATCTCC TTCTTAAAGT TAAACAAAAT TATTTCTAGA GGGGAATTGT TATCCGCTCA

 2401 CAATTCCCCT ATAGTGAGTC GTATTAATTT CGCGGGATCG AGATCGATCT CGATCCTCTA

 2461 CGCCGGACGC ATCGTGGCCG GCATCACCGG CGCCACAGGT GCGGTTGCTG GCGCCTATAT

 2521 CGCCGACATC ACCGATGGGG AAGATCGGGC TCGCCACTTC GGGCTCATGA GCGCTTGTTT

 2581 CGGCGTGGGT ATGGTGGCAG GCCCCGTGGC CGGGGGACTG TTGGGCGCCA TCTCCTTGCA

 2641 TGCACCATTC CTTGCGGCGG CGGTGCTCAA CGGCCTCAAC CTACTACTGG GCTGCTTCCT

 2701 AATGCAGGAG TCGCATAAGG GAGAGCGTCG AGATCCCGGA CACCATCGAA TGGCGCAAAA

 2761 CCTTTCGCGG TATGGCATGA TAGCGCCCGG AAGAGAGTCA ATTCAGGGTG GTGAATGTGA

 2821 AACCAGTAAC GTTATACGAT GTCGCAGAGT ATGCCGGTGT CTCTTATCAG ACCGTTTCCC

 2881 GCGTGGTGAA CCAGGCCAGC CACGTTTCTG CGAAAACGCG GGAAAAAGTG GAAGCGGCGA

 2941 TGGCGGAGCT GAATTACATT CCCAACCGCG TGGCACAACA ACTGGCGGGC AAACAGTCGT

 3001 TGCTGATTGG CGTTGCCACC TCCAGTCTGG CCCTGCACGC GCCGTCGCAA ATTGTCGCGG

 3061 CGATTAAATC TCGCGCCGAT CAACTGGGTG CCAGCGTGGT GGTGTCGATG GTAGAACGAA

 3121 GCGGCGTCGA AGCCTGTAAA GCGGCGGTGC ACAATCTTCT CGCGCAACGC GTCAGTGGGC

 3181 TGATCATTAA CTATCCGCTG GATGACCAGG ATGCCATTGC TGTGGAAGCT GCCTGCACTA

 3241 ATGTTCCGGC GTTATTTCTT GATGTCTCTG ACCAGACACC CATCAACAGT ATTATTTTCT

 3301 CCCATGAAGA CGGTACGCGA CTGGGCGTGG AGCATCTGGT CGCATTGGGT CACCAGCAAA

 3361 TCGCGCTGTT AGCGGGCCCA TTAAGTTCTG TCTCGGCGCG TCTGCGTCTG GCTGGCTGGC

 3421 ATAAATATCT CACTCGCAAT CAAATTCAGC CGATAGCGGA ACGGGAAGGC GACTGGAGTG

 3481 CCATGTCCGG TTTTCAACAA ACCATGCAAA TGCTGAATGA GGGCATCGTT CCCACTGCGA

 3541 TGCTGGTTGC CAACGATCAG ATGGCGCTGG GCGCAATGCG CGCCATTACC GAGTCCGGGC

 3601 TGCGCGTTGG TGCGGACATC TCGGTAGTGG GATACGACGA TACCGAAGAC AGCTCATGTT

 3661 ATATCCCGCC GTTAACCACC ATCAAACAGG ATTTTCGCCT GCTGGGGCAA ACCAGCGTGG

 3721 ACCGCTTGCT GCAACTCTCT CAGGGCCAGG CGGTGAAGGG CAATCAGCTG TTGCCCGTCT

 3781 CACTGGTGAA AAGAAAAACC ACCCTGGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT

 3841 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG

 3901 CGCAACGCAA TTAATGTAAG TTAGCTCACT CATTAGGCAC CGGGATCTCG ACCGATGCCC

 3961 TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC GGGGCATGAC TAGCATGATC

 4021 GTGCTCCTGT CGTTGAGGAC CCGGCTAGGC TGGCGGGGTT GCCTTACTGG TTAGCAGAAT

 4081 GAATCACCGA TACGCGAGCG AACGTGAAGC GACTGCTGCT GCAAAACGTC TGCGACCTGA

 4141 GCAACAACAT GAATGGTCTT CGGTTTCCGT GTTTCGTAAA GTCTGGAAAC GCGGAAGTCA

 4201 GCGCCCTGCA CCATTATGTT CCGGATCTGC ATCGCAGGAT GCTGCTGGCT ACCCTGTGGA

 4261 ACACCTACAT CTGTATTAAC GAAGCGCTGG CATTGACCCT GAGTGATTTT TCTCTGGTCC

 4321 CGCCGCATCC ATACCGCCAG TTGTTTACCC TCACAACGTT CCAGTAACCG GGCATGTTCA

 4381 TCATCAGTAA CCCGTATCGT GAGCATCCTC TCTCGTTTCA TCGGTATCAT TACCCCCATG

 4441 AACAGAAATC CCCCTTACAC GGAGGCATCA GTGACCAAAC AGGAAAAAAC CGCCCTTAAC

 4501 ATGGCCCGCT TTATCAGAAG CCAGACATTA ACGCTTCTGG AGAAACTCAA CGAGCTGGAC

 4561 GCGGATGAAC AGGCAGACAT CTGTGAATCG CTTCACGACC ACGCTGATGA GCTTTACCGC

 4621 AGCTGCCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG

 4681 ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA

 4741 GCGGGTGTTG GCGGGTGTCG GGGCGCAGCC ATGACCCAGT CACGTAGCGA TAGCGGAGTG

 4801 TATACTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATATGCG

 4861 GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCT CTTCCGCTTC

 4921 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC

 4981 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC

 5041 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG

 5101 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC

 5161 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT

 5221 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT

 5281 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG

 5341 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT

 5401 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT

 5461 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG

 5521 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA

 5581 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT

 5641 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC

 5701 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGACCTA

 5761 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT

 5821 AAATGCTTCA ATCATGATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC

 5881 CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG

 5941 AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC

 6001 AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT

 6061 TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC

 6121 GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG

 6181 CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT

 6241 AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT

 6301 TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA

 6361 GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC

 6421 AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG

 6481 GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT

 6541 GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA

 6601 GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT

 6661 GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA

 6721 GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG

 6781 ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGCCATCGT GCCGCCAGAA ATTGTAAACG

 6841 TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT

 6901 AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG

 6961 TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC

 7021 GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT

 7081 TGGGGTCGAG GTGCCGTAAA GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG

 7141 CTTGACGGGG AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG

 7201 GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC

 7261 TTAATGCGCC GCTACAGGGC GCGTCCCATT CGCCA

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pET-43.1 EK/LIC其他大肠杆菌表达载体：