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pTrc99a

编号	载体名称
北京华越洋生物VECT5460	pTrc99a

pTrc 99a载体基本信息

别名:	pTrc99a, pTrc 99a
质粒类型:	大肠杆菌蛋白表达载体
拷贝水平:	高拷贝
启动子:	Trc
克隆方法:	多克隆位点，限制性内切酶
载体大小:	4176 bp
5' 测序引物及序列:	RV-M: GAGCGGATAACAATTTCACACAGG
3' 测序引物及序列:	pTrcHis-R: GATTTAATCTGTATCAGG
载体标签:	none
载体抗性:	氨苄
备注:	宿主菌: E.coli. 相关载体: pBR322, pTrc97.
稳定性:	瞬时表达
组成型:	组成型
病毒/非病毒:	非病毒

pTrc 99a载体质粒图谱和多克隆位点信息

pTrc 99a载体简介

细菌表达，可诱导蛋白表达，使用IPTG进行诱导（1 mM IPTG）。

pTrc 99a载体序列

ORIGIN

1 GTTTGACAGC TTATCATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC

61 GGAAGCTGTG GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC

121 GCACTCCCGT TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC

181 TGAAATGAGC TGTTGACAAT TAATCATCCG GCTCGTATAA TGTGTGGAAT TGTGAGCGGA

241 TAACAATTTC ACACAGGAAA CAGACCATGG AATTCGAGCT CGGTACCCGG GGATCCTCTA

301 GAGTCGACCT GCAGGCATGC AAGCTTGGCT GTTTTGGCGG ATGAGAGAAG ATTTTCAGCC

361 TGATACAGAT TAAATCAGAA CGCAGAAGCG GTCTGATAAA ACAGAATTTG CCTGGCGGCA

421 GTAGCGCGGT GGTCCCACCT GACCCCATGC CGAACTCAGA AGTGAAACGC CGTAGCGCCG

481 ATGGTAGTGT GGGGTCTCCC CATGCGAGAG TAGGGAACTG CCAGGCATCA AATAAAACGA

541 AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC

601 CTGAGTAGGA CAAATCCGCC GGGAGCGGAT TTGAACGTTG CGAAGCAACG GCCCGGAGGG

661 TGGCGGGCAG GACGCCCGCC ATAAACTGCC AGGCATCAAA TTAAGCAGAA GGCCATCCTG

721 ACGGATGGCC TTTTTGCGTT TCTACAAACT CTTTTTGTTT ATTTTTCTAA ATACATTCAA

781 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA

841 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC

901 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG

961 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC

1021 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT

1081 TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG

1141 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG

1201 AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA

1261 CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC

1321 GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA

1381 CGATGCCTAC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC

1441 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC

1501 TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG

1561 GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA

1621 TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG

1681 GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA

1741 TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC

1801 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA

1861 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA

1921 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC

1981 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT

2041 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC

2101 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC

2161 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA

2221 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG

2281 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG

2341 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT

2401 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT

2461 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC

2521 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT

2581 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG

2641 CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA

2701 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC

2761 GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC

2821 GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG

2881 GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCAGATCAA

2941 TTCGCGCGCG AAGGCGAAGC GGCATGCATT TACGTTGACA CCATCGAATG GTGCAAAACC

3001 TTTCGCGGTA TGGCATGATA GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT GAATGTGAAA

3061 CCAGTAACGT TATACGATGT CGCAGAGTAT GCCGGTGTCT CTTATCAGAC CGTTTCCCGC

3121 GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA AGCGGCGATG

3181 GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA ACAGTCGTTG

3241 CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT TGTCGCGGCG

3301 ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT AGAACGAAGC

3361 GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT CAGTGGGCTG

3421 ATCATTAACT ATCCGCTGGA TGACCAGGAT GCCATTGCTG TGGAAGCTGC CTGCACTAAT

3481 GTTCCGGCGT TATTTCTTGA TGTCTCTGAC CAGACACCCA TCAACAGTAT TATTTTCTCC

3541 CATGAAGACG GTACGCGACT GGGCGTGGAG CATCTGGTCG CATTGGGTCA CCAGCAAATC

3601 GCGCTGTTAG CGGGCCCATT AAGTTCTGTC TCGGCGCGTC TGCGTCTGGC TGGCTGGCAT

3661 AAATATCTCA CTCGCAATCA AATTCAGCCG ATAGCGGAAC GGGAAGGCGA CTGGAGTGCC

3721 ATGTCCGGTT TTCAACAAAC CATGCAAATG CTGAATGAGG GCATCGTTCC CACTGCGATG

3781 CTGGTTGCCA ACGATCAGAT GGCGCTGGGC GCAATGCGCG CCATTACCGA GTCCGGGCTG

3841 CGCGTTGGTG CGGATATCTC GGTAGTGGGA TACGACGATA CCGAAGACAG CTCATGTTAT

3901 ATCCCGCCGT CAACCACCAT CAAACAGGAT TTTCGCCTGC TGGGGCAAAC CAGCGTGGAC

3961 CGCTTGCTGC AACTCTCTCA GGGCCAGGCG GTGAAGGGCA ATCAGCTGTT GCCCGTCTCA

4021 CTGGTGAAAA GAAAAACCAC CCTGGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG

4081 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG

4141 CAACGCAATT AATGTGAGTT AGCGCGAATT GATCTG

其他大肠杆菌表达载体：

pKD13	PinPoint Xa-1	pKD4	pTf16
pTWIN2	pTYB11	pKJE7	pET-17b
pTrcHis2 A	pBad/gIII B	pGEX-6P-2	pG-KJE8
pET-46 EK/LIC	pMal-p2G	pGEX-2TK	pGEX-4T-3
pET-49b(+)	pEZZ18	pMal-c2X	pET300/NT-DEST
pBAD-TOPO	pMal-p2X	pET-33b(+)	pRSET-CFP
pET-23(+)	pMal-c4X	pET-24c(+)	pTrcHis2 B
pET-11b(+)	pET-41a(+)	pET-24(+)	pET-44c(+)
pQE-82L	pGEX-4T-1	pMal-p4X	pET-43.1c(+)
pQE-9	pALEX a,b,c	pBad/gIII C	pET-27b(+)
pQE-70	pKD20	pET102/D-TOPO	pET-21a(+)
pET-16b	pRSET B	pACYCDuet-1	pET-20b(+)
pET-21d(+)	pCOLADuet-1	pCYB1	pET-14b
pRSET-EmGFP	pET-42b(+)	pGEX-5X-2	pET-26b(+)
pET301/CT-DEST	pGEX-6P-1	pET-28c(+)	pET-50b(+)
pGro7	pRSET A	pET-32a(+)	pMal-c2G
pET-11a(+)	pRSET C	ptdTomato	pQE-30
pET-12b(+)	pET-42c(+)	pET-43.1b(+)	pET-41c(+)
pET-23b(+)	pET-39b(+)	pBV220	pET-41c(+)
pBad/His C	pET-32b(+)	pCold IV	pETDuet-1
pBAD/Thio-TOPO	pET-29c(+)	pE-SUMO	pTYB12
pCold-ProS2	pET-24b(+)	pACYC184	pEGM-7ZF(+)
pCold III	pBAD33	pBad/Myc-His A	pSP73
pCold-GST	pAmCyan	PinPoint Xa-2	pTXB1
pET-52b(+)	pColdS-SUMO	pTWIN1	pBad/gIII A
pET-23d(+)	pCold II	pET-24a(+)	pkk223-3
pET-29a(+)	pBAD102/D-TOPO	pET-28a(+)	pBAD18
pET-30 EK/LIC	pBad/Myc-His C	pProEX HTc	pET-44 EK/LIC
pET-32 Xa/LIC	pBad/His B	pMal-p2E	pTrcHis B
pET-40b(+)	pET-23a(+)	pQE-60	pGEX-3X
pET-45b(+)	pET-12c(+)	pQE-32	pTrc99a
pMal-c2E	pET-11c(+)	pBad24	pACYC177
pGEX-5X-1	pQE-80L	pET-12a(+)	pMXB10
pGEX-6P-3	pQE-31	pET-23c(+)	pGEX-KG
pTrcHis A	pET-51b(+)	pBad/His A	pTrcHis C
pGEX-2T	pET-47b(+)	pBad/Myc-His B	pET-3a(+)
pEcoli-6xHN-GFPuv	pET-22b(+)	pBAD202/D-TOPO	pGEX-5X-3
pBR322	pET-19b	pCold I	pET-32 EK/LIC
pET-28b(+)	pET-21b(+)	pCold TF	pET-30 Xa/LIC
pGEX-4T-2	pET-25b(+)	pQE-81L	pET-29b(+)
pET-31b(+)	pET-42a(+)	pQE-16	pGEM-T
pET-43.1 EK/LIC	pET-44a(+)	pQE-40	pBlueScript SK(+)
pMAL-c5x	pTrcHis2 C	pET-48b(+)	pET-SUMO
pkk232-8	pET302/NT-His	pET-21b(+)	pCDFDuet-1
pET-5a(+)	pGFPuv	pET-15b	pBluescript II SK(+)
pET-5b(+)	pBad43	pET-21c(+)	pProEX HTb
pSP64	pG-Tf2	pET-24d(+)	pKD46
PinPoint Xa-3	pSE380	pET-30a(+)	pBluescript II KS(-)
pMAL-p5e	pMAL-p5x	pET-43.1a(+)	pEGM-11ZF(+)
pTYB2	pET-30b(+)	pET-37b(+)	pDsRed-Express2
pKD3	pProEX HTa	pET303/CT-His	pBV221
pSUMO	pTYB1	pRSET-BFP	pET-11d(+)
pET-3b(+)	pET-41 EK/LIC	pET-32c(+)	pET-44b(+)
pRSFDuet-1	pET-30c(+)	pET-His	pET-41b(+)