北京华越洋生物
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首页    载体及质粒    pKD20
2

pKD20

pKD20

pKD20

 

编号

载体名称

北京华越洋生物VECT4940

pKD20

 

pKD20载体基本信息

载体名称:

pKD20

质粒类型:

细菌表达;基因敲除系统

高拷贝/低拷贝:

--

启动子:

--

克隆方法:

多克隆位点,限制性内切酶

载体大小:

6078  bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

--

筛选标记:

--

备注:

--

稳定性:

--

组成型:

--

病毒/非病毒:

--

 

pKD20载体质粒图谱和多克隆位点信息

 


  

pKD20载体简介

pKD20载体是Red系统敲除载体,复制子是pSC101 ori,质粒大小是6078bp,带有AmpicillinAmp)抗性,可转化进相应大肠杆菌宿主,通过抗生素抗性筛选大量复制扩增,培养条件是LB30℃。

pKD20载体启动子有araBAD,标签有araC

pKD20载体5´测序引物:根据序列设计,3´测序引物:根据序列设计。

 

pKD20载体序列

ORIGIN     

        1 CATCGATTTA TTATGACAAC TTGACGGCTA CATCATTCAC TTTTTCTTCA CAACCGGCAC

       61 GGAACTCGCT CGGGCTGGCC CCGGTGCATT TTTTAAATAC CCGCGAGAAA TAGAGTTGAT

      121 CGTCAAAACC AACATTGCGA CCGACGGTGG CGATAGGCAT CCGGGTGGTG CTCAAAAGCA

      181 GCTTCGCCTG GCTGATACGT TGGTCCTCGC GCCAGCTTAA GACGCTAATC CCTAACTGCT

      241 GGCGGAAAAG ATGTGACAGA CGCGACGGCG ACAAGCAAAC ATGCTGTGCG ACGCTGGCGA

      301 TATCAAAATT GCTGTCTGCC AGGTGATCGC TGATGTACTG ACAAGCCTCG CGTACCCGAT

      361 TATCCATCGG TGGATGGAGC GACTCGTTAA TCGCTTCCAT GCGCCGCAGT AACAATTGCT

      421 CAAGCAGATT TATCGCCAGC AGCTCCGAAT AGCGCCCTTC CCCTTGCCCG GCGTTAATGA

      481 TTTGCCCAAA CAGGTCGCTG AAATGCGGCT GGTGCGCTTC ATCCGGGCGA AAGAACCCCG

      541 TATTGGCAAA TATTGACGGC CAGTTAAGCC ATTCATGCCA GTAGGCGCGC GGACGAAAGT

      601 AAACCCACTG GTGATACCAT TCGCGAGCCT CCGGATGACG ACCGTAGTGA TGAATCTCTC

      661 CTGGCGGGAA CAGCAAAATA TCACCCGGTC GGCAAACAAA TTCTCGTCCC TGATTTTTCA

      721 CCACCCCCTG ACCGCGAATG GTGAGATTGA GAATATAACC TTTCATTCCC AGCGGTCGGT

      781 CGATAAAAAA ATCGAGATAA CCGTTGGCCT CAATCGGCGT TAAACCCGCC ACCAGATGGG

      841 CATTAAACGA GTATCCCGGC AGCAGGGGAT CATTTTGCGC TTCAGCCATA CTTTTCATAC

      901 TCCCGCCATT CAGAGAAGAA ACCAATTGTC CATATTGCAT CAGACATTGC CGTCACTGCG

      961 TCTTTTACTG GCTCTTCTCG CTAACCAAAC CGGTAACCCC GCTTATTAAA AGCATTCTGT

     1021 AACAAAGCGG GACCAAAGCC ATGACAAAAA CGCGTAACAA AAGTGTCTAT AATCACGGCA

     1081 GAAAAGTCCA CATTGATTAT TTGCACGGCG TCACACTTTG CTATGCCATA GCATTTTTAT

     1141 CCATAAGATT AGCGGATCCT ACCTGACGCT TTTTATCGCA ACTCTCTACT GTTTCTCCAT

     1201 ACCCGTTTTT TTGGGAATTC GAGCTCTAAG GAGGTTATAA AAAATGGATA TTAATACTGA

     1261 AACTGAGATC AAGCAAAAGC ATTCACTAAC CCCCTTTCCT GTTTTCCTAA TCAGCCCGGC

     1321 ATTTCGCGGG CGATATTTTC ACAGCTATTT CAGGAGTTCA GCCATGAACG CTTATTACAT

     1381 TCAGGATCGT CTTGAGGCTC AGAGCTGGGC GCGTCACTAC CAGCAGCTCG CCCGTGAAGA

     1441 GAAAGAGGCA GAACTGGCAG ACGACATGGA AAAAGGCCTG CCCCAGCACC TGTTTGAATC

     1501 GCTATGCATC GATCATTTGC AACGCCACGG GGCCAGCAAA AAATCCATTA CCCGTGCGTT

     1561 TGATGACGAT GTTGAGTTTC AGGAGCGCAT GGCAGAACAC ATCCGGTACA TGGTTGAAAC

     1621 CATTGCTCAC CACCAGGTTG ATATTGATTC AGAGGTATAA AACGAATGAG TACTGCACTC

     1681 GCAACGCTGG CTGGGAAGCT GGCTGAACGT GTCGGCATGG ATTCTGTCGA CCCACAGGAA

     1741 CTGATCACCA CTCTTCGCCA GACGGCATTT AAAGGTGATG CCAGCGATGC GCAGTTCATC

     1801 GCATTACTGA TCGTTGCCAA CCAGTACGGC CTTAATCCGT GGACGAAAGA AATTTACGCC

     1861 TTTCCTGATA AGCAGAATGG CATCGTTCCG GTGGTGGGCG TTGATGGCTG GTCCCGCATC

     1921 ATCAATGAAA ACCAGCAGTT TGATGGCATG GACTTTGAGC AGGACAATGA ATCCTGTACA

     1981 TGCCGGATTT ACCGCAAGGA CCGTAATCAT CCGATCTGCG TTACCGAATG GATGGATGAA

     2041 TGCCGCCGCG AACCATTCAA AACTCGCGAA GGCAGAGAAA TCACGGGGCC GTGGCAGTCG

     2101 CATCCCAAAC GGATGTTACG TCATAAAGCC ATGATTCAGT GTGCCCGTCT GGCCTTCGGA

     2161 TTTGCTGGTA TCTATGACAA GGATGAAGCC GAGCGCATTG TCGAAAATAC TGCATACACT

     2221 GCAGAACGTC AGCCGGAACG CGACATCACT CCGGTTAACG ATGAAACCAT GCAGGAGATT

     2281 AACACTCTGC TGATCGCCCT GGATAAAACA TGGGATGACG ACTTATTGCC GCTCTGTTCC

     2341 CAGATATTTC GCCGCGACAT TCGTGCATCG TCAGAACTGA CACAGGCCGA AGCAGTAAAA

     2401 GCTCTTGGAT TCCTGAAACA GAAAGCCGCA GAGCAGAAGG TGGCAGCATG ACACCGGACA

     2461 TTATCCTGCA GCGTACCGGG ATCGATGTGA GAGCTGTCGA ACAGGGGGAT GATGCGTGGC

     2521 ACAAATTACG GCTCGGCGTC ATCACCGCTT CAGAAGTTCA CAACGTGATA GCAAAACCCC

     2581 GCTCCGGAAA GAAGTGGCCT GACATGAAAA TGTCCTACTT CCACACCCTG CTTGCTGAGG

     2641 TTTGCACCGG TGTGGCTCCG GAAGTTAACG CTAAAGCACT GGCCTGGGGA AAACAGTACG

     2701 AGAACGACGC CAGAACCCTG TTTGAATTCA CTTCCGGCGT GAATGTTACT GAATCCCCGA

     2761 TCATCTATCG CGACGAAAGT ATGCGTACCG CCTGCTCTCC CGATGGTTTA TGCAGTGACG

     2821 GCAACGGCCT TGAACTGAAA TGCCCGTTTA CCTCCCGGGA TTTCATGAAG TTCCGGCTCG

     2881 GTGGTTTCGA GGCCATAAAG TCAGCTTACA TGGCCCAGGT GCAGTACAGC ATGTGGGTGA

     2941 CGCGAAAAAA TGCCTGGTAC TTTGCCAACT ATGACCCGCG TATGAAGCGT GAAGGCCTGC

     3001 ATTATGTCGT GATTGAGCGG GATGAAAAGT ACATGGCGAG TTTTGACGAG ATCGTGCCGG

     3061 AGTTCATCGA AAAAATGGAC GAGGCACTGG CTGAAATTGG TTTTGTATTT GGGGAGCAAT

     3121 GGCGATGATA AGCTAGCCCA TGGGTATGGA CAGTTTTCCC TTTGATATGT AACGGTGAAC

     3181 AGTTGTTCTA CTTTTGTTTG TTAGTCTTGA TGCTTCACTG ATAGATACAA GAGCCATAAG

     3241 AACCTCAGAT CCTTCCGTAT TTAGCCAGTA TGTTCTCTAG TGTGGTTCGT TGTTTTTGCG

     3301 TGAGCCATGA GAACGAACCA TTGAGATCAT ACTTACTTTG CATGTCACTC AAAAATTTTG

     3361 CCTCAAAACT GGTGAGCTGA ATTTTTGCAG TTAAAGCATC GTGTAGTGTT TTTCTTAGTC

     3421 CGTTACGTAG GTAGGAATCT GATGTAATGG TTGTTGGTAT TTTGTCACCA TTCATTTTTA

     3481 TCTGGTTGTT CTCAAGTTCG GTTACGAGAT CCATTTGTCT ATCTAGTTCA ACTTGGAAAA

     3541 TCAACGTATC AGTCGGGCGG CCTCGCTTAT CAACCACCAA TTTCATATTG CTGTAAGTGT

     3601 TTAAATCTTT ACTTATTGGT TTCAAAACCC ATTGGTTAAG CCTTTTAAAC TCATGGTAGT

     3661 TATTTTCAAG CATTAACATG AACTTAAATT CATCAAGGCT AATCTCTATA TTTGCCTTGT

     3721 GAGTTTTCTT TTGTGTTAGT TCTTTTAATA ACCACTCATA AATCCTCATA GAGTATTTGT

     3781 TTTCAAAAGA CTTAACATGT TCCAGATTAT ATTTTATGAA TTTTTTTAAC TGGAAAAGAT

     3841 AAGGCAATAT CTCTTCACTA AAAACTAATT CTAATTTTTC GCTTGAGAAC TTGGCATAGT

     3901 TTGTCCACTG GAAAATCTCA AAGCCTTTAA CCAAAGGATT CCTGATTTCC ACAGTTCTCG

     3961 TCATCAGCTC TCTGGTTGCT TTAGCTAATA CACCATAAGC ATTTTCCCTA CTGATGTTCA

     4021 TCATCTGAGC GTATTGGTTA TAAGTGAACG ATACCGTCCG TTCTTTCCTT GTAGGGTTTT

     4081 CAATCGTGGG GTTGAGTAGT GCCACACAGC ATAAAATTAG CTTGGTTTCA TGCTCCGTTA

     4141 AGTCATAGCG ACTAATCGCT AGTTCATTTG CTTTGAAAAC AACTAATTCA GACATACATC

     4201 TCAATTGGTC TAGGTGATTT TAATCACTAT ACCAATTGAG ATGGGCTAGT CAATGATAAT

     4261 TACTAGTCCT TTTCCTTTGA GTTGTGGGTA TCTGTAAATT CTGCTAGACC TTTGCTGGAA

     4321 AACTTGTAAA TTCTGCTAGA CCCTCTGTAA ATTCCGCTAG ACCTTTGTGT GTTTTTTTTG

     4381 TTTATATTCA AGTGGTTATA ATTTATAGAA TAAAGAAAGA ATAAAAAAAG ATAAAAAGAA

     4441 TAGATCCCAG CCCTGTGTAT AACTCACTAC TTTAGTCAGT TCCGCAGTAT TACAAAAGGA

     4501 TGTCGCAAAC GCTGTTTGCT CCTCTACAAA ACAGACCTTA AAACCCTAAA GGCTTAAGTA

     4561 GCACCCTCGC AAGCTCGGTT GCGGCCGCAA TCGGGCAAAT CGCTGAATAT TCCTTTTGTC

     4621 TCCGACCATC AGGCACCTGA GTCGCTGTCT TTTTCGTGAC ATTCAGTTCG CTGCGCTCAC

     4681 GGCTCTGGCA GTGAATGGGG GTAAATGGCA CTACAGGCGC CTTTTATGGA TTCATGCAAG

     4741 GAAACTACCC ATAATACAAG AAAAGCCCGT CACGGGCTTC TCAGGGCGTT TTATGGCGGG

     4801 TCTGCTATGT GGTGCTATCT GACTTTTTGC TGTTCAGCAG TTCCTGCCCT CTGATTTTCC

     4861 AGTCTGACCA CTTCGGATTA TCCCGTGACA GGTCATTCAG ACTGGCTAAT GCACCCAGTA

     4921 AGGCAGCGGT ATCATCAACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA

     4981 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA

     5041 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA

     5101 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC

     5161 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA

     5221 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA

     5281 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT

     5341 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG

     5401 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC

     5461 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG

     5521 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG

     5581 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT

     5641 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT

     5701 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC

     5761 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC

     5821 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG

     5881 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA

     5941 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA

     6001 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC

     6061 CCCGAAAAGT GCCACCTG

//

 

 

其他大肠杆菌表达载体:

pKD13

PinPoint Xa-1

pKD4

pTf16

pTWIN2

pTYB11

pKJE7

pET-17b

pTrcHis2 A

pBad/gIII B

pGEX-6P-2

pG-KJE8

pET-46 EK/LIC

pMal-p2G

pGEX-2TK

pGEX-4T-3

pET-49b(+)

pEZZ18

pMal-c2X

pET300/NT-DEST

pBAD-TOPO

pMal-p2X

pET-33b(+)

pRSET-CFP

pET-23(+)

pMal-c4X

pET-24c(+)

pTrcHis2 B

pET-11b(+)

pET-41a(+)

pET-24(+)

pET-44c(+)

pQE-82L

pGEX-4T-1

pMal-p4X

pET-43.1c(+)

pQE-9

pALEX a,b,c

pBad/gIII C

pET-27b(+)

pQE-70

pKD20

pET102/D-TOPO

pET-21a(+)

pET-16b

pRSET B

pACYCDuet-1

pET-20b(+)

pET-21d(+)

pCOLADuet-1

pCYB1

pET-14b

pRSET-EmGFP

pET-42b(+)

pGEX-5X-2

pET-26b(+)

pET301/CT-DEST

pGEX-6P-1

pET-28c(+)

pET-50b(+)

pGro7

pRSET A

pET-32a(+)

pMal-c2G

pET-11a(+)

pRSET C

ptdTomato

pQE-30

pET-12b(+)

pET-42c(+)

pET-43.1b(+)

pET-41c(+)

pET-23b(+)

pET-39b(+)

pBV220

pET-41c(+)

pBad/His C

pET-32b(+)

pCold IV

pETDuet-1

pBAD/Thio-TOPO

pET-29c(+)

pE-SUMO

pTYB12

pCold-ProS2

pET-24b(+)

pACYC184

pEGM-7ZF(+)

pCold III

pBAD33

pBad/Myc-His A

pSP73

pCold-GST

pAmCyan

PinPoint Xa-2

pTXB1

pET-52b(+)

pColdS-SUMO

pTWIN1

pBad/gIII A

pET-23d(+)

pCold II

pET-24a(+)

pkk223-3

pET-29a(+)

pBAD102/D-TOPO

pET-28a(+)

pBAD18

pET-30 EK/LIC

pBad/Myc-His C

pProEX HTc

pET-44 EK/LIC

pET-32 Xa/LIC

pBad/His B

pMal-p2E

pTrcHis B

pET-40b(+)

pET-23a(+)

pQE-60

pGEX-3X

pET-45b(+)

pET-12c(+)

pQE-32

pTrc99a

pMal-c2E

pET-11c(+)

pBad24

pACYC177

pGEX-5X-1

pQE-80L

pET-12a(+)

pMXB10

pGEX-6P-3

pQE-31

pET-23c(+)

pGEX-KG

pTrcHis A

pET-51b(+)

pBad/His A

pTrcHis C

pGEX-2T

pET-47b(+)

pBad/Myc-His B

pET-3a(+)

pEcoli-6xHN-GFPuv

pET-22b(+)

pBAD202/D-TOPO

pGEX-5X-3

pBR322

pET-19b

pCold I

pET-32 EK/LIC

pET-28b(+)

pET-21b(+)

pCold TF

pET-30 Xa/LIC

pGEX-4T-2

pET-25b(+)

pQE-81L

pET-29b(+)

pET-31b(+)

pET-42a(+)

pQE-16

pGEM-T

pET-43.1 EK/LIC

pET-44a(+)

pQE-40

pBlueScript SK(+)

pMAL-c5x

pTrcHis2 C

pET-48b(+)

pET-SUMO

pkk232-8

pET302/NT-His

pET-21b(+)

pCDFDuet-1

pET-5a(+)

pGFPuv

pET-15b

pBluescript II SK(+)

pET-5b(+)

pBad43

pET-21c(+)

pProEX HTb

pSP64

pG-Tf2

pET-24d(+)

pKD46

PinPoint Xa-3

pSE380

pET-30a(+)

pBluescript II KS(-)

pMAL-p5e

pMAL-p5x

pET-43.1a(+)

pEGM-11ZF(+)

pTYB2

pET-30b(+)

pET-37b(+)

pDsRed-Express2

pKD3

pProEX HTa

pET303/CT-His

pBV221

pSUMO

pTYB1

pRSET-BFP

pET-11d(+)

pET-3b(+)

pET-41 EK/LIC

pET-32c(+)

pET-44b(+)

pRSFDuet-1

pET-30c(+)

pET-His

pET-41b(+)