pGEX-4T-1
pGEX-4T-1
编号 | 载体名称 |
北京华越洋生物VECT4940 | pGEX-4T-1 |
pGEX4T1载体基本信息
别名: | pGEX-4T-1, pGEX4T1, pGEX 4T 1 |
质粒类型: | 大肠杆菌蛋白表达载体 |
表达水平: | 高拷贝 |
启动子: | Tac |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 4969 bp |
5' 测序引物及序列: | pGEX5': GGGCTGGCAAGCCACGTTTGGTG |
3' 测序引物及序列: | pGEX3': CCGGGAGCTGCATGTGTCAGAGG |
载体标签: | N-GST |
载体抗性: | Ampicillin 氨苄 |
备注: | 复制子是pMB1，限制性内切酶酶切位点有 BamHI,EcoRI SmaI,Sal I,Xho I,Not I等，可转化进大肠杆菌中高拷贝表达。pGEX-4T-1载体是一种常用的原核表达载体，该载体多克隆位点区域含有多个常用的内切酶位点序列，便于不同基因的克隆；且全序列中含有tac启动子、GST标签序列，是一种高效的蛋白表达载体。Tac启动子在没有诱导物存在的情况下，质粒上携带的lacIq基因产物可以有效抑制tac启动子的转录。可在BL21、Rosstea等表达菌株中高表达融合有GST标签的蛋白，且用凝血酶可将目的蛋白的GST标签切掉，便于下游蛋白纯化。 |
稳定性: | 瞬时表达 Transient |
组成型: | 诱导表达 |
病毒/非病毒: | 非病毒 |
pGEX4T1载体质粒图谱和多克隆位点信息
pGEX4T1载体简介
There are a range of Gluthatione Sepharose™ prepacked column and bulk media products available to purify GST Fusion proteins. For manual purification of sample volumes up to 600 µl use GST SpinTrap™ microspin columns or GST MultiTrap™ 4B 96-well plates. For sample volumes between 600 µl and 10 ml use GST GraviTrap™ gravity flow column. Where sample volumes are above 10 ml, use LabMate™ reservoir together with GST GraviTrap. All formats described can be used for preparation of samples in parallel. In addition GST HiTrap™ 1 and 5 ml columns and GST HiPrep™ FF 16/10 column are available for purification in a chromatography system such as the ÄKTA™ design system. Alternatively, Gluthatione Sepharose bulk media are available from 10 ml up to 500 ml. A GST Bulk Kit is also available combining 10 ml Gluthatione Sepharose 4B bulk medium and 5 empty columns with required buffers.
pGEX系列载体：
pGEX-1λT EcoRI/BAP
pGEX-2T
pGEX-2TK
pGEX-20T
pGEX-3X
pGEX-4T-1
pGEX-4T-2
pGEX-4T-3
pGEX-5X-1
pGEX-5X-2
pGEX-5X-3
pGEX-6P-1
pGEX-6P-2
pGEX-6P-3
pGEX-KG
pGEX4T1载体序列
ORIGIN
1 ACGTTATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC GGAAGCTGTG
61 GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC GCACTCCCGT
121 TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC TGAAATGAGC
181 TGTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATT GTGAGCGGAT AACAATTTCA
241 CACAGGAAAC AGTATTCATG TCCCCTATAC TAGGTTATTG GAAAATTAAG GGCCTTGTGC
301 AACCCACTCG ACTTCTTTTG GAATATCTTG AAGAAAAATA TGAAGAGCAT TTGTATGAGC
361 GCGATGAAGG TGATAAATGG CGAAACAAAA AGTTTGAATT GGGTTTGGAG TTTCCCAATC
421 TTCCTTATTA TATTGATGGT GATGTTAAAT TAACACAGTC TATGGCCATC ATACGTTATA
481 TAGCTGACAA GCACAACATG TTGGGTGGTT GTCCAAAAGA GCGTGCAGAG ATTTCAATGC
541 TTGAAGGAGC GGTTTTGGAT ATTAGATACG GTGTTTCGAG AATTGCATAT AGTAAAGACT
601 TTGAAACTCT CAAAGTTGAT TTTCTTAGCA AGCTACCTGA AATGCTGAAA ATGTTCGAAG
661 ATCGTTTATG TCATAAAACA TATTTAAATG GTGATCATGT AACCCATCCT GACTTCATGT
721 TGTATGACGC TCTTGATGTT GTTTTATACA TGGACCCAAT GTGCCTGGAT GCGTTCCCAA
781 AATTAGTTTG TTTTAAAAAA CGTATTGAAG CTATCCCACA AATTGATAAG TACTTGAAAT
841 CCAGCAAGTA TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC
901 ATCCTCCAAA ATCGGATCTG GTTCCGCGTG GATCCCCGGA ATTCCCGGGT CGACTCGAGC
961 GGCCGCATCG TGACTGACTG ACGATCTGCC TCGCGCGTTT CGGTGATGAC GGTGAAAACC
1021 TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA
1081 GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC
1141 AGTCACGTAG CGATAGCGGA GTGTATAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC
1201 TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC
1261 GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC
1321 CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA
1381 GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT
1441 TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG
1501 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG
1561 AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG
1621 TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG
1681 AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA
1741 GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG
1801 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC
1861 GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG
1921 CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC
1981 GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG
2041 CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG
2101 GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA
2161 CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC
2221 TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA
2281 AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA
2341 AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG
2401 GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC
2461 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA
2521 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC
2581 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG
2641 TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC
2701 CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC
2761 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC
2821 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA
2881 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC
2941 TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG
3001 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT
3061 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA
3121 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC
3181 GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATAAATTCCG
3241 ACACCATCGA ATGGTGCAAA ACCTTTCGCG GTATGGCATG ATAGCGCCCG GAAGAGAGTC
3301 AATTCAGGGT GGTGAATGTG AAACCAGTAA CGTTATACGA TGTCGCAGAG TATGCCGGTG
3361 TCTCTTATCA GACCGTTTCC CGCGTGGTGA ACCAGGCCAG CCACGTTTCT GCGAAAACGC
3421 GGGAAAAAGT GGAAGCGGCG ATGGCGGAGC TGAATTACAT TCCCAACCGC GTGGCACAAC
3481 AACTGGCGGG CAAACAGTCG TTGCTGATTG GCGTTGCCAC CTCCAGTCTG GCCCTGCACG
3541 CGCCGTCGCA AATTGTCGCG GCGATTAAAT CTCGCGCCGA TCAACTGGGT GCCAGCGTGG
3601 TGGTGTCGAT GGTAGAACGA AGCGGCGTCG AAGCCTGTAA AGCGGCGGTG CACAATCTTC
3661 TCGCGCAACG CGTCAGTGGG CTGATCATTA ACTATCCGCT GGATGACCAG GATGCCATTG
3721 CTGTGGAAGC TGCCTGCACT AATGTTCCGG CGTTATTTCT TGATGTCTCT GACCAGACAC
3781 CCATCAACAG TATTATTTTC TCCCATGAAG ACGGTACGCG ACTGGGCGTG GAGCATCTGG
3841 TCGCATTGGG TCACCAGCAA ATCGCGCTGT TAGCGGGCCC ATTAAGTTCT GTCTCGGCGC
3901 GTCTGCGTCT GGCTGGCTGG CATAAATATC TCACTCGCAA TCAAATTCAG CCGATAGCGG
3961 AACGGGAAGG CGACTGGAGT GCCATGTCCG GTTTTCAACA AACCATGCAA ATGCTGAATG
4021 AGGGCATCGT TCCCACTGCG ATGCTGGTTG CCAACGATCA GATGGCGCTG GGCGCAATGC
4081 GCGCCATTAC CGAGTCCGGG CTGCGCGTTG GTGCGGATAT CTCGGTAGTG GGATACGACG
4141 ATACCGAAGA CAGCTCATGT TATATCCCGC CGTTAACCAC CATCAAACAG GATTTTCGCC
4201 TGCTGGGGCA AACCAGCGTG GACCGCTTGC TGCAACTCTC TCAGGGCCAG GCGGTGAAGG
4261 GCAATCAGCT GTTGCCCGTC TCACTGGTGA AAAGAAAAAC CACCCTGGCG CCCAATACGC
4321 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC
4381 GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA
4441 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA
4501 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGGAT TCACTGGCCG TCGTTTTACA
4561 ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC
4621 TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG
4681 CAGCCTGAAT GGCGAATGGC GCTTTGCCTG GTTTCCGGCA CCAGAAGCGG TGCCGGAAAG
4741 CTGGCTGGAG TGCGATCTTC CTGAGGCCGA TACTGTCGTC GTCCCCTCAA ACTGGCAGAT
4801 GCACGGTTAC GATGCGCCCA TCTACACCAA CGTAACCTAT CCCATTACGG TCAATCCGCC
4861 GTTTGTTCCC ACGGAGAATC CGACGGGTTG TTACTCGCTC ACATTTAATG TTGATGAAAG
4921 CTGGCTACAG GAAGGCCAGA CGCGAATTAT TTTTGATGGC GTTGGAATT
//
其他大肠杆菌表达载体：
