pET-24(+)
pET-24(+)
编号 | 名称 |
北京华越洋VECT-640 | pET-24(+) |
pET24+载体基本信息
别名: | pET24(+), pet 24(+) |
质粒类型: | 大肠杆菌蛋白表达 |
表达水平: | 高 |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 5236 bp |
5' 测序引物: | T7 |
5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
载体标签: | C-His |
载体抗性: | Kana |
备注: | Designed for expression from bacterial translational signal within cloned insert (no ribosome binding site or ATG start codon). |
稳定性: | 瞬时表达 Transient |
组成型: | 组成型 Constitutive |
病毒/非病毒: | 非病毒 |
pET24+载体质粒图谱和多克隆位点信息
Feature Name | Start | End |
T7_terminator | 129 | 1 |
T7_Terminal_primer | 69 | 87 |
lacO | 235 | 208 |
T7_promoter | 253 | 235 |
tet (300 - 563) | 285 | 548 |
pBRrevBam_primer | 356 | 337 |
lacI | 631 | 1722 |
tet (576 - 851) | 1781 | 2056 |
ROP | 2531 | 2722 |
pGEX_3_primer | 2738 | 2716 |
pBR322_origin | 3756 | 3137 |
KanR2 | 3862 | 4677 |
ORF | Start | End |
ORF frame 3 | 390 | 890 |
ORF frame 1 | 763 | 1722 |
ORF frame 1 | 3862 | 4677 |
Enzyme Name | Cut |
XhoI | 158 |
NotI | 166 |
EagI | 166 |
HindIII | 173 |
SalI | 179 |
SacI | 190 |
EcoRI | 192 |
BamHI | 198 |
BglII | 268 |
BclI | 1004 |
ApaI | 1201 |
EcoRV | 1440 |
HpaI | 1496 |
FspI | 2072 |
NruI | 3950 |
ClaI | 3984 |
XmaI | 4165 |
SmaI | 4167 |
pET24+载体简介
pET-24(+) is a transcription vector designed for expression from bacterial translation signals carried within a cloned insert. It therefore lacks the ribosome binding site and ATG start codon present on the pET translation vectors. A C-terminal His•Tag® sequence is available. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer.
pET24+载体序列
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tcctagaggg gaattgttat ccgctcacaa ttcccctata
241 gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctacgccgga cgcatcgtgg
301 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
361 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
421 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
481 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
541 agggagagcg tcgagatccc ggacaccatc gaatggcgca aaacctttcg cggtatggca
601 tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgttatac
661 gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc
721 agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac
781 attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc
841 acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc
901 gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt
961 aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg
1021 ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt
1081 cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg
1141 cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc
1201 ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc
1261 aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa
1321 caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat
1381 cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat
1441 atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc
1501 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc
1561 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa
1621 accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
1681 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
1741 aagttagctc actcattagg caccgggatc tcgaccgatg cccttgagag ccttcaaccc
1801 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
1861 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga
1921 ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca
1981 cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc
2041 cattatcgcc ggcatggcgg ccccacgggt gcgcatgatc gtgctcctgt cgttgaggac
2101 ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg
2161 aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt
2221 cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt
2281 ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac
2341 gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag
2401 ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt
2461 gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc ccccttacac
2521 ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag
2581 ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat
2641 ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg
2701 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
2761 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
2821 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
2881 gcatcagagc agattgtact gagagtgcac catatatgcg gtgtgaaata ccgcacagat
2941 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
3001 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
3061 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
3121 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
3181 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
3241 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
3301 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
3361 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
3421 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
3481 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
3541 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
3601 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
3661 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
3721 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
3781 ggaacgaaaa ctcacgttaa gggattttgg tcatgaacaa taaaactgtc tgcttacata
3841 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcttg ctctaggccg
3901 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
3961 gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt
4021 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
4081 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
4141 gcatggttac tcaccactgc gatccccggg aaaacagcat tccaggtatt agaagaatat
4201 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
4261 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
4321 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
4381 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
4441 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
4501 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
4561 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
4621 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaagaa
4681 ttaattcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
4741 gcgcacattt ccccgaaaag tgccacctga aattgtaaac gttaatattt tgttaaaatt
4801 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
4861 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
4921 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
4981 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
5041 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
5101 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
5161 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
5221 cgcgtcccat tcgcca
//
其他大肠杆菌表达载体：
