pKD3
pKD3
编号 | 载体名称 |
北京华越洋生物VECT4290 | pKD3 |
pKD3载体基本信息
载体名称: | pKD3 |
质粒类型: | 细菌表达；基因敲除系统 |
高拷贝/低拷贝: | -- |
启动子: | R6K |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 2804 bp |
5' 测序引物及序列: | R6K-3 |
3' 测序引物及序列: | rrnB-T1-term-F |
载体标签: | -- |
载体抗性: | Ampicillin 和 Chloramphenicol |
筛选标记: | -- |
备注: | -- |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | -- |
pKD3载体质粒图谱和多克隆位点信息
pKD3载体简介：
This is a template plasmid for FRT-flanked cat cassette. The cat cassette originally came from pSC140. pKD3 and pKD4 are identical except for the region between the FRT sites, so they create an identical scar that has stop codons in all six reading frames. As drawn in the map, this scar has an idealized ribosome binding site and start codon for downstream (rightward) gene expression.2.
pKD3载体序列
ORIGIN
1 AGATTGCAGC ATTACACGTC TTGAGCGATT GTGTAGGCTG GAGCTGCTTC GAAGTTCCTA
61 TACTTTCTAG AGAATAGGAA CTTCGGAATA GGAACTTCAT TTAAATGGCG CGCCTTACGC
121 CCCGCCCTGC CACTCATCGC AGTACTGTTG TATTCATTAA GCATCTGCCG ACATGGAAGC
181 CATCACAAAC GGCATGATGA ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG
241 TATAATATTT GCCCATGGTG AAAACGGGGG CGAAGAAGTT GTCCATATTG GCCACGTTTA
301 AATCAAAACT GGTGAAACTC ACCCAGGGAT TGGCTGAGAC GAAAAACATA TTCTCAATAA
361 ACCCTTTAGG GAAATAGGCC AGGTTTTCAC CGTAACACGC CACATCTTGC GAATATATGT
421 GTAGAAACTG CCGGAAATCG TCGTGGTATT CACTCCAGAG CGATGAAAAC GTTTCAGTTT
481 GCTCATGGAA AACGGTGTAA CAAGGGTGAA CACTATCCCA TATCACCAGC TCACCGTCTT
541 TCATTGCCAT ACGTAATTCC GGATGAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG
601 CCGGATAAAA CTTGTGCTTA TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA ATATCCAGCT
661 GAACGGTCTG GTTATAGGTA CATTGAGCAA CTGACTGAAA TGCCTCAAAA TGTTCTTTAC
721 GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC ATTTTAGCTT
781 CCTTAGCTCC TGAAAATCTC GACAACTCAA AAAATACGCC CGGTAGTGAT CTTATTTCAT
841 TATGGTGAAA GTTGGAACCT CTTACGTGCC GATCAACGTC TCATTTTCGC CAAAAGTTGG
901 CCCAGGGCTT CCCGGTATCA ACAGGGACAC CAGGATTTAT TTATTCTGCG AAGTGATCTT
961 CCGTCACAGG TAGGCGCGCC GAAGTTCCTA TACTTTCTAG AGAATAGGAA CTTCGGAATA
1021 GGAACTAAGG AGGATATTCA TATGGACCAT GGCTAATTCC CATGTCAGCC GTTAAGTGTT
1081 CCTGTGTCAC TGAAAATTGC TTTGAGAGGC TCTAAGGGCT TCTCAGTGCG TTACATCCCT
1141 GGCTTGTTGT CCACAACCGT TAAACCTTAA AAGCTTTAAA AGCCTTATAT ATTCTTTTTT
1201 TTCTTATAAA ACTTAAAACC TTAGAGGCTA TTTAAGTTGC TGATTTATAT TAATTTTATT
1261 GTTCAAACAT GAGAGCTTAG TACGTGAAAC ATGAGAGCTT AGTACGTTAG CCATGAGAGC
1321 TTAGTACGTT AGCCATGAGG GTTTAGTTCG TTAAACATGA GAGCTTAGTA CGTTAAACAT
1381 GAGAGCTTAG TACGTGAAAC ATGAGAGCTT AGTACGTACT ATCAACAGGT TGAACTGCGG
1441 ATCTTGCGGC CGCAAAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
1501 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT
1561 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG
1621 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA
1681 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
1741 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
1801 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT
1861 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT
1921 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
1981 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC
2041 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT
2101 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG
2161 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT
2221 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
2281 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA
2341 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG
2401 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
2461 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGCAT CGATGGCCCC
2521 CCGATGGTAG TGTGGGGTCT CCCCATGCGA GAGTAGGGAA CTGCCAGGCA TCAAATAAAA
2581 CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT
2641 CTCCTGAGTA GGACAAATCC GCCGGGAGCG GATTTGAACG TTGCGAAGCA ACGGCCCGGA
2701 GGGTGGCGGG CAGGACGCCC GCCATAAACT GCCAGGCATC AAATTAAGCA GAAGGCCATC
2761 CTGACGGATG GCCTTTTTGC GTGGCCAGTG CCAAGCTTGC ATGC
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pKD3其他大肠杆菌表达载体：
