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pQE-30

编号	载体名称
北京华越洋生物VECT5540	pQE-30

pQE30载体基本信息

别名:	pQE30, pQE 30
质粒类型:	大肠杆菌蛋白表达载体
表达水平:	低拷贝
启动子:	T5
克隆方法:	多克隆位点，限制性内切酶
载体大小:	3461 bp
5' 测序引物及序列:	pQE30-F: AGCGGATAACAATTTCACACAG
3' 测序引物及序列:	pQE30-R: TTCTGAGGTCATTACTGGATC
载体标签:	N-His
载体抗性:	Ampicillin
备注:	pQE vectors and constructs can be maintained in any E. coli strain that is ampicillin-sensitive and carries the pREP4 repressor plasmid, or harbors the lacIq gene on the F-factor episome.
稳定性:	瞬时表达 Transient
组成型:	组成型 Constitutive
病毒/非病毒:	非病毒

pQE30载体质粒图谱和多克隆位点信息

pQE30载体简介

pQE-30载体的多克隆位点MCS有BanII，SacI，Acc651，SmaI等酶切位点，可采用双酶切连接方法连接目的基因

pQE30载体序列

ORIGIN
1 CTCGAGAAAT CATAAAAAAT TTATTTGCTT TGTGAGCGGA TAACAATTAT AATAGATTCA
61 ATTGTGAGCG GATAACAATT TCACACAGAA TTCATTAAAG AGGAGAAATT AACTATGAGA
121 GGATCGCATC ACCATCACCA TCACGGATCC GCATGCGAGC TCGGTACCCC GGGTCGACCT
181 GCAGCCAAGC TTAATTAGCT GAGCTTGGAC TCCTGTTGAT AGATCCAGTA ATGACCTCAG
241 AACTCCATCT GGATTTGTTC AGAACGCTCG GTTGCCGCCG GGCGTTTTTT ATTGGTGAGA
301 ATCCAAGCTA GCTTGGCGAG ATTTTCAGGA GCTAAGGAAG CTAAAATGGA GAAAAAAATC
361 ACTGGATATA CCACCGTTGA TATATCCCAA TGGCATCGTA AAGAACATTT TGAGGCATTT
421 CAGTCAGTTG CTCAATGTAC CTATAACCAG ACCGTTCAGC TGGATATTAC GGCCTTTTTA
481 AAGACCGTAA AGAAAAATAA GCACAAGTTT TATCCGGCCT TTATTCACAT TCTTGCCCGC
541 CTGATGAATG CTCATCCGGA ATTTCGTATG GCAATGAAAG ACGGTGAGCT GGTGATATGG
601 GATAGTGTTC ACCCTTGTTA CACCGTTTTC CATGAGCAAA CTGAAACGTT TTCATCGCTC
661 TGGAGTGAAT ACCACGACGA TTTCCGGCAG TTTCTACACA TATATTCGCA AGATGTGGCG
721 TGTTACGGTG AAAACCTGGC CTATTTCCCT AAAGGGTTTA TTGAGAATAT GTTTTTCGTC
781 TCAGCCAATC CCTGGGTGAG TTTCACCAGT TTTGATTTAA ACGTGGCCAA TATGGACAAC
841 TTCTTCGCCC CCGTTTTCAC CATGGGCAAA TATTATACGC AAGGCGACAA GGTGCTGATG
901 CCGCTGGCGA TTCAGGTTCA TCATGCCGTT TGTGATGGCT TCCATGTCGG CAGAATGCTT
961 AATGAATTAC AACAGTACTG CGATGAGTGG CAGGGCGGGG CGTAATTTTT TTAAGGCAGT
1021 TATTGGTGCC CTTAAACGCC TGGGGTAATG ACTCTCTAGC TTGAGGCATC AAATAAAACG
1081 AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT TGTTTGTCGG TGAACGCTCT
1141 CCTGAGTAGG ACAAATCCGC CCTCTAGAGC TGCCTCGCGC GTTTCGGTGA TGACGGTGAA
1201 AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG
1261 AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CGCAGCCATG
1321 ACCCAGTCAC GTAGCGATAG CGGAGTGTAT ACTGGCTTAA CTATGCGGCA TCAGAGCAGA
1381 TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT
1441 ACCGCATCAG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC
1501 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG
1561 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG
1621 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC
1681 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG
1741 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT
1801 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG
1861 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT
1921 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC
1981 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT
2041 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC
2101 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA
2161 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT
2221 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC
2281 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT
2341 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC
2401 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG
2461 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG
2521 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC
2581 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA
2641 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG
2701 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT
2761 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA
2821 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG
2881 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA
2941 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT
3001 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA
3061 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT
3121 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT
3181 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA
3241 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT
3301 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC
3361 GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA
3421 CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTTCA C
//

其他大肠杆菌表达载体：

pKD13	PinPoint Xa-1	pKD4	pTf16
pTWIN2	pTYB11	pKJE7	pET-17b
pTrcHis2 A	pBad/gIII B	pGEX-6P-2	pG-KJE8
pET-46 EK/LIC	pMal-p2G	pGEX-2TK	pGEX-4T-3
pET-49b(+)	pEZZ18	pMal-c2X	pET300/NT-DEST
pBAD-TOPO	pMal-p2X	pET-33b(+)	pRSET-CFP
pET-23(+)	pMal-c4X	pET-24c(+)	pTrcHis2 B
pET-11b(+)	pET-41a(+)	pET-24(+)	pET-44c(+)
pQE-82L	pGEX-4T-1	pMal-p4X	pET-43.1c(+)
pQE-9	pALEX a,b,c	pBad/gIII C	pET-27b(+)
pQE-70	pKD20	pET102/D-TOPO	pET-21a(+)
pET-16b	pRSET B	pACYCDuet-1	pET-20b(+)
pET-21d(+)	pCOLADuet-1	pCYB1	pET-14b
pRSET-EmGFP	pET-42b(+)	pGEX-5X-2	pET-26b(+)
pET301/CT-DEST	pGEX-6P-1	pET-28c(+)	pET-50b(+)
pGro7	pRSET A	pET-32a(+)	pMal-c2G
pET-11a(+)	pRSET C	ptdTomato	pQE-30
pET-12b(+)	pET-42c(+)	pET-43.1b(+)	pET-41c(+)
pET-23b(+)	pET-39b(+)	pBV220	pET-41c(+)
pBad/His C	pET-32b(+)	pCold IV	pETDuet-1
pBAD/Thio-TOPO	pET-29c(+)	pE-SUMO	pTYB12
pCold-ProS2	pET-24b(+)	pACYC184	pEGM-7ZF(+)
pCold III	pBAD33	pBad/Myc-His A	pSP73
pCold-GST	pAmCyan	PinPoint Xa-2	pTXB1
pET-52b(+)	pColdS-SUMO	pTWIN1	pBad/gIII A
pET-23d(+)	pCold II	pET-24a(+)	pkk223-3
pET-29a(+)	pBAD102/D-TOPO	pET-28a(+)	pBAD18
pET-30 EK/LIC	pBad/Myc-His C	pProEX HTc	pET-44 EK/LIC
pET-32 Xa/LIC	pBad/His B	pMal-p2E	pTrcHis B
pET-40b(+)	pET-23a(+)	pQE-60	pGEX-3X
pET-45b(+)	pET-12c(+)	pQE-32	pTrc99a
pMal-c2E	pET-11c(+)	pBad24	pACYC177
pGEX-5X-1	pQE-80L	pET-12a(+)	pMXB10
pGEX-6P-3	pQE-31	pET-23c(+)	pGEX-KG
pTrcHis A	pET-51b(+)	pBad/His A	pTrcHis C
pGEX-2T	pET-47b(+)	pBad/Myc-His B	pET-3a(+)
pEcoli-6xHN-GFPuv	pET-22b(+)	pBAD202/D-TOPO	pGEX-5X-3
pBR322	pET-19b	pCold I	pET-32 EK/LIC
pET-28b(+)	pET-21b(+)	pCold TF	pET-30 Xa/LIC
pGEX-4T-2	pET-25b(+)	pQE-81L	pET-29b(+)
pET-31b(+)	pET-42a(+)	pQE-16	pGEM-T
pET-43.1 EK/LIC	pET-44a(+)	pQE-40	pBlueScript SK(+)
pMAL-c5x	pTrcHis2 C	pET-48b(+)	pET-SUMO
pkk232-8	pET302/NT-His	pET-21b(+)	pCDFDuet-1
pET-5a(+)	pGFPuv	pET-15b	pBluescript II SK(+)
pET-5b(+)	pBad43	pET-21c(+)	pProEX HTb
pSP64	pG-Tf2	pET-24d(+)	pKD46
PinPoint Xa-3	pSE380	pET-30a(+)	pBluescript II KS(-)
pMAL-p5e	pMAL-p5x	pET-43.1a(+)	pEGM-11ZF(+)
pTYB2	pET-30b(+)	pET-37b(+)	pDsRed-Express2
pKD3	pProEX HTa	pET303/CT-His	pBV221
pSUMO	pTYB1	pRSET-BFP	pET-11d(+)
pET-3b(+)	pET-41 EK/LIC	pET-32c(+)	pET-44b(+)
pRSFDuet-1	pET-30c(+)	pET-His	pET-41b(+)