넳 넲

pET-5a(+)

编号	载体名称
北京华越洋生物VECT4350	pET-5a(+)

pet5a载体基本信息

质粒类型:	未知
克隆方法:	多克隆位点
载体大小:	4134
备注:	宿主菌: E.coli. 相关载体: pBR322.
稳定性:	瞬时表达
组成型/诱导型:	诱导表达
病毒/非病毒:	非病毒

pet5a载体质粒图谱和多克隆位点信息

pet5a载体序列

ORIGIN

1 TTCGGATCCG CGACCCATTT GCTGTCCACC AGTCATGCTA GCCATATGTA TATCTCCTTC

61 TTAAAGTTAA ACAAAATTAT TTCTAGAGGG AAACCGTTGT GGTCTCCCTA TAGTGAGTCG

121 TATTAATTTC GCGGGATCGA GATCTCGATC CTCTACGCCG GACGCATCGT GGCCGGCATC

181 ACCGGCGCCA CAGGTGCGGT TGCTGGCGCC TATATCGCCG ACATCACCGA TGGGGAAGAT

241 CGGGCTCGCC ACTTCGGGCT CATGAGCGCT TGTTTCGGCG TGGGTATGGT GGCAGGCCCC

301 GTGGCCGGGG GACTGTTGGG CGCCATCTCC TTGCATGCAC CATTCCTTGC GGCGGCGGTG

361 CTCAACGGCC TCAACCTACT ACTGGGCTGC TTCCTAATGC AGGAGTCGCA TAAGGGAGAG

421 CGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG GGCGCGGGGC

481 ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA TGCAACTCGT AGGACAGGTG

541 CCGGCAGCGC TCTGGGTCAT TTTCGGCGAG GACCGCTTTC GCTGGAGCGC GACGATGATC

601 GGCCTGTCGC TTGCGGTATT CGGAATCTTG CACGCCCTCG CTCAAGCCTT CGTCACTGGT

661 CCCGCCACCA AACGTTTCGG CGAGAAGCAG GCCATTATCG CCGGCATGGC GGCCGACGCG

721 CTGGGCTACG TCTTGCTGGC GTTCGCGACG CGAGGCTGGA TGGCCTTCCC CATTATGATT

781 CTTCTCGCTT CCGGCGGCAT CGGGATGCCC GCGTTGCAGG CCATGCTGTC CAGGCAGGTA

841 GATGACGACC ATCAGGGACA GCTTCAAGGA TCGCTCGCGG CTCTTACCAG CCTAACTTCG

901 ATCACTGGAC CGCTGATCGT CACGGCGATT TATGCCGCCT CGGCGAGCAC ATGGAACGGG

961 TTGGCATGGA TTGTAGGCGC CGCCCTATAC CTTGTCTGCC TCCCCGCGTT GCGTCGCGGT

1021 GCATGGAGCC GGGCCACCTC GACCTGAATG GAAGCCGGCG GCACCTCGCT AACGGATTCA

1081 CCACTCCAAG AATTGGAGCC AATCAATTCT TGCGGAGAAC TGTGAATGCG CAAACCAACC

1141 CTTGGCAGAA CATATCCATC GCGTCCGCCA TCTCCAGCAG CCGCACGCGG CGCATCTCGG

1201 GCAGCGTTGG GTCCTGGCCA CGGGTGCGCA TGATCGTGCT CCTGTCGTTG AGGACCCGGC

1261 TAGGCTGGCG GGGTTGCCTT ACTGGTTAGC AGAATGAATC ACCGATACGC GAGCGAACGT

1321 GAAGCGACTG CTGCTGCAAA ACGTCTGCGA CCTGAGCAAC AACATGAATG GTCTTCGGTT

1381 TCCGTGTTTC GTAAAGTCTG GAAACGCGGA AGTCAGCGCC CTGCACCATT ATGTTCCGGA

1441 TCTGCATCGC AGGATGCTGC TGGCTACCCT GTGGAACACC TACATCTGTA TTAACGAAGC

1501 GCTGGCATTG ACCCTGAGTG ATTTTTCTCT GGTCCCGCCG CATCCATACC GCCAGTTGTT

1561 TACCCTCACA ACGTTCCAGT AACCGGGCAT GTTCATCATC AGTAACCCGT ATCGTGAGCA

1621 TCCTCTCTCG TTTCATCGGT ATCATTACCC CCATGAACAG AAATCCCCCT TACACGGAGG

1681 CATCAGTGAC CAAACAGGAA AAAACCGCCC TTAACATGGC CCGCTTTATC AGAAGCCAGA

1741 CATTAACGCT TCTGGAGAAA CTCAACGAGC TGGACGCGGA TGAACAGGCA GACATCTGTG

1801 AATCGCTTCA CGACCACGCT GATGAGCTTT ACCGCAGCTG CCTCGCGCGT TTCGGTGATG

1861 ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG

1921 ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG

1981 CAGCCATGAC CCAGTCACGT AGCGATAGCG GAGTGTATAC TGGCTTAACT ATGCGGCATC

2041 AGAGCAGATT GTACTGAGAG TGCACCATAT ATGCGGTGTG AAATACCGCA CAGATGCGTA

2101 AGGAGAAAAT ACCGCATCAG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG

2161 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA

2221 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC

2281 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC

2341 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG

2401 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC

2461 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT

2521 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG

2581 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC

2641 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT

2701 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT

2761 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC

2821 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA

2881 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC

2941 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC

3001 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT

3061 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA

3121 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT

3181 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA

3241 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC

3301 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG

3361 CGCAACGTTG TTGCCATTGC TGCAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT

3421 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA

3481 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA

3541 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC

3601 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG

3661 AGTTGCTCTT GCCCGGCGTC AACACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA

3721 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG

3781 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC

3841 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG

3901 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT

3961 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA

4021 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC

4081 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTTCA AGAA

pET-5a(+)其他大肠杆菌表达载体：

pKD13	PinPoint Xa-1	pKD4	pTf16
pTWIN2	pTYB11	pKJE7	pET-17b
pTrcHis2 A	pBad/gIII B	pGEX-6P-2	pG-KJE8
pET-46 EK/LIC	pMal-p2G	pGEX-2TK	pGEX-4T-3
pET-49b(+)	pEZZ18	pMal-c2X	pET300/NT-DEST
pBAD-TOPO	pMal-p2X	pET-33b(+)	pRSET-CFP
pET-23(+)	pMal-c4X	pET-24c(+)	pTrcHis2 B
pET-11b(+)	pET-41a(+)	pET-24(+)	pET-44c(+)
pQE-82L	pGEX-4T-1	pMal-p4X	pET-43.1c(+)
pQE-9	pALEX a,b,c	pBad/gIII C	pET-27b(+)
pQE-70	pKD20	pET102/D-TOPO	pET-21a(+)
pET-16b	pRSET B	pACYCDuet-1	pET-20b(+)
pET-21d(+)	pCOLADuet-1	pCYB1	pET-14b
pRSET-EmGFP	pET-42b(+)	pGEX-5X-2	pET-26b(+)
pET301/CT-DEST	pGEX-6P-1	pET-28c(+)	pET-50b(+)
pGro7	pRSET A	pET-32a(+)	pMal-c2G
pET-11a(+)	pRSET C	ptdTomato	pQE-30
pET-12b(+)	pET-42c(+)	pET-43.1b(+)	pET-41c(+)
pET-23b(+)	pET-39b(+)	pBV220	pET-41c(+)
pBad/His C	pET-32b(+)	pCold IV	pETDuet-1
pBAD/Thio-TOPO	pET-29c(+)	pE-SUMO	pTYB12
pCold-ProS2	pET-24b(+)	pACYC184	pEGM-7ZF(+)
pCold III	pBAD33	pBad/Myc-His A	pSP73
pCold-GST	pAmCyan	PinPoint Xa-2	pTXB1
pET-52b(+)	pColdS-SUMO	pTWIN1	pBad/gIII A
pET-23d(+)	pCold II	pET-24a(+)	pkk223-3
pET-29a(+)	pBAD102/D-TOPO	pET-28a(+)	pBAD18
pET-30 EK/LIC	pBad/Myc-His C	pProEX HTc	pET-44 EK/LIC
pET-32 Xa/LIC	pBad/His B	pMal-p2E	pTrcHis B
pET-40b(+)	pET-23a(+)	pQE-60	pGEX-3X
pET-45b(+)	pET-12c(+)	pQE-32	pTrc99a
pMal-c2E	pET-11c(+)	pBad24	pACYC177
pGEX-5X-1	pQE-80L	pET-12a(+)	pMXB10
pGEX-6P-3	pQE-31	pET-23c(+)	pGEX-KG
pTrcHis A	pET-51b(+)	pBad/His A	pTrcHis C
pGEX-2T	pET-47b(+)	pBad/Myc-His B	pET-3a(+)
pEcoli-6xHN-GFPuv	pET-22b(+)	pBAD202/D-TOPO	pGEX-5X-3
pBR322	pET-19b	pCold I	pET-32 EK/LIC
pET-28b(+)	pET-21b(+)	pCold TF	pET-30 Xa/LIC
pGEX-4T-2	pET-25b(+)	pQE-81L	pET-29b(+)
pET-31b(+)	pET-42a(+)	pQE-16	pGEM-T
pET-43.1 EK/LIC	pET-44a(+)	pQE-40	pBlueScript SK(+)
pMAL-c5x	pTrcHis2 C	pET-48b(+)	pET-SUMO
pkk232-8	pET302/NT-His	pET-21b(+)	pCDFDuet-1
pET-5a(+)	pGFPuv	pET-15b	pBluescript II SK(+)
pET-5b(+)	pBad43	pET-21c(+)	pProEX HTb
pSP64	pG-Tf2	pET-24d(+)	pKD46
PinPoint Xa-3	pSE380	pET-30a(+)	pBluescript II KS(-)
pMAL-p5e	pMAL-p5x	pET-43.1a(+)	pEGM-11ZF(+)
pTYB2	pET-30b(+)	pET-37b(+)	pDsRed-Express2
pKD3	pProEX HTa	pET303/CT-His	pBV221
pSUMO	pTYB1	pRSET-BFP	pET-11d(+)
pET-3b(+)	pET-41 EK/LIC	pET-32c(+)	pET-44b(+)
pRSFDuet-1	pET-30c(+)	pET-His	pET-41b(+)