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1

pET-11b(+)

pET-11b(+)

pET-11b(+)

 

编号

名称

北京华越洋VECT-180

pET-11b(+)

 

pet11b载体基本信息

载体名称:

pET-11b

质粒类型:

大肠杆菌表达载体

高拷贝/低拷贝:

低拷贝

克隆方法:

多克隆位点;限制性内切酶

启动子:

T7/lac

载体大小:

5676 bp

5' 测序引物及序列:

T7 Fwd:  5'd[TAATACGACTCACTATAGGG]3'

3' 测序引物及序列:

--

载体标签:

T7 Tag (N-末端)

载体抗性:

氨苄青霉素(Ampicillin

克隆菌株:

DH5a

表达菌株:

BL21系列

备注:

pET-11a,b,c之间仅阅读框(读码框)不同,彼此相差1bppET-11d载体的多克隆位点处不含NdeI切割位点。

稳定性:

稳表达

组成型/诱导型:

诱导型(IPTG)

病毒/非病毒:

非病毒

 

pet11b载体质粒图谱和多克隆位点信息

 

pet11b载体简介

 

pET-11a-d载体具有NT7标签和BamH I多克隆克隆位点。这些载体是很多pET家族载体的前身。pET-21a-d(+)系列载体分别对应于pET-11a-d载体,但是包含有几个额外的特征序列。独特的酶切位点在载体图谱中被标注出来。需要注意的是,这些载体序列是依据载体pBR322的方向来进行编号的,所以T7标签表达区域在载体图谱上是反向的。

 

pet11b载体序列

ORIGIN

    1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa

   61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg

  121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt

  181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag

  241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt

  301 cgggctttgt tagcagccgg atcccgaccc atttgctgtc caccagtcat gctagccata

  361 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc

  421 acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc

  481 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc

  541 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg

  601 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc

  661 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat

  721 gcaggagtcg cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct

  781 ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac

  841 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg

  901 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg

  961 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc

 1021 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga

 1081 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg

 1141 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga

 1201 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg

 1261 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc

 1321 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg

 1381 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata

 1441 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca

 1501 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc

 1561 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc

 1621 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata

 1681 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc

 1741 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac

 1801 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg

 1861 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc

 1921 aacgcaatta atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg

 1981 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca

 2041 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc

 2101 attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta

 2161 ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc

 2221 ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg

 2281 gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc

 2341 atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga

 2401 cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg accgctgatc

 2461 gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc

 2521 gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc

 2581 tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag

 2641 ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca

 2701 tcgcgtccgc catctccagc agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc

 2761 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc

 2821 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca

 2881 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc

 2941 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct

 3001 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag

 3061 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca

 3121 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg

 3181 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg

 3241 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga

 3301 aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg

 3361 ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac

 3421 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag

 3481 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac

 3541 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag

 3601 agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc

 3661 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga

 3721 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca

 3781 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg

 3841 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt

 3901 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc

 3961 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct

 4021 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc

 4081 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta

 4141 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca

 4201 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag

 4261 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag

 4321 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt

 4381 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa

 4441 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg

 4501 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga

 4561 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta

 4621 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc

 4681 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg

 4741 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga

 4801 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt

 4861 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt

 4921 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc

 4981 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc

 5041 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca

 5101 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag

 5161 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg

 5221 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa

 5281 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa

 5341 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga

 5401 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga

 5461 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg

 5521 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt

 5581 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa

 5641 aataggcgta tcacgaggcc ctttcgtctt caagaa

//

 

其他大肠杆菌表达载体:

pKD13

PinPoint Xa-1

pKD4

pTf16

pTWIN2

pTYB11

pKJE7

pET-17b

pTrcHis2 A

pBad/gIII B

pGEX-6P-2

pG-KJE8

pET-46 EK/LIC

pMal-p2G

pGEX-2TK

pGEX-4T-3

pET-49b(+)

pEZZ18

pMal-c2X

pET300/NT-DEST

pBAD-TOPO

pMal-p2X

pET-33b(+)

pRSET-CFP

pET-23(+)

pMal-c4X

pET-24c(+)

pTrcHis2 B

pET-11b(+)

pET-41a(+)

pET-24(+)

pET-44c(+)

pQE-82L

pGEX-4T-1

pMal-p4X

pET-43.1c(+)

pQE-9

pALEX a,b,c

pBad/gIII C

pET-27b(+)

pQE-70

pKD20

pET102/D-TOPO

pET-21a(+)

pET-16b

pRSET B

pACYCDuet-1

pET-20b(+)

pET-21d(+)

pCOLADuet-1

pCYB1

pET-14b

pRSET-EmGFP

pET-42b(+)

pGEX-5X-2

pET-26b(+)

pET301/CT-DEST

pGEX-6P-1

pET-28c(+)

pET-50b(+)

pGro7

pRSET A

pET-32a(+)

pMal-c2G

pET-11a(+)

pRSET C

ptdTomato

pQE-30

pET-12b(+)

pET-42c(+)

pET-43.1b(+)

pET-41c(+)

pET-23b(+)

pET-39b(+)

pBV220

pET-41c(+)

pBad/His C

pET-32b(+)

pCold IV

pETDuet-1

pBAD/Thio-TOPO

pET-29c(+)

pE-SUMO

pTYB12

pCold-ProS2

pET-24b(+)

pACYC184

pEGM-7ZF(+)

pCold III

pBAD33

pBad/Myc-His A

pSP73

pCold-GST

pAmCyan

PinPoint Xa-2

pTXB1

pET-52b(+)

pColdS-SUMO

pTWIN1

pBad/gIII A

pET-23d(+)

pCold II

pET-24a(+)

pkk223-3

pET-29a(+)

pBAD102/D-TOPO

pET-28a(+)

pBAD18

pET-30 EK/LIC

pBad/Myc-His C

pProEX HTc

pET-44 EK/LIC

pET-32 Xa/LIC

pBad/His B

pMal-p2E

pTrcHis B

pET-40b(+)

pET-23a(+)

pQE-60

pGEX-3X

pET-45b(+)

pET-12c(+)

pQE-32

pTrc99a

pMal-c2E

pET-11c(+)

pBad24

pACYC177

pGEX-5X-1

pQE-80L

pET-12a(+)

pMXB10

pGEX-6P-3

pQE-31

pET-23c(+)

pGEX-KG

pTrcHis A

pET-51b(+)

pBad/His A

pTrcHis C

pGEX-2T

pET-47b(+)

pBad/Myc-His B

pET-3a(+)

pEcoli-6xHN-GFPuv

pET-22b(+)

pBAD202/D-TOPO

pGEX-5X-3

pBR322

pET-19b

pCold I

pET-32 EK/LIC

pET-28b(+)

pET-21b(+)

pCold TF

pET-30 Xa/LIC

pGEX-4T-2

pET-25b(+)

pQE-81L

pET-29b(+)

pET-31b(+)

pET-42a(+)

pQE-16

pGEM-T

pET-43.1 EK/LIC

pET-44a(+)

pQE-40

pBlueScript SK(+)

pMAL-c5x

pTrcHis2 C

pET-48b(+)

pET-SUMO

pkk232-8

pET302/NT-His

pET-21b(+)

pCDFDuet-1

pET-5a(+)

pGFPuv

pET-15b

pBluescript II SK(+)

pET-5b(+)

pBad43

pET-21c(+)

pProEX HTb

pSP64

pG-Tf2

pET-24d(+)

pKD46

PinPoint Xa-3

pSE380

pET-30a(+)

pBluescript II KS(-)

pMAL-p5e

pMAL-p5x

pET-43.1a(+)

pEGM-11ZF(+)

pTYB2

pET-30b(+)

pET-37b(+)

pDsRed-Express2

pKD3

pProEX HTa

pET303/CT-His

pBV221

pSUMO

pTYB1

pRSET-BFP

pET-11d(+)

pET-3b(+)

pET-41 EK/LIC

pET-32c(+)

pET-44b(+)

pRSFDuet-1

pET-30c(+)

pET-His

pET-41b(+)