pET-46 EK/LIC

pET46 EK/LIC载体基本信息

pET46 EK/LIC载体质粒图谱和多克隆位点信息

pET46 EK/LIC载体简介

The pET-46 Ek/LIC vector is prepared for rapid, directional cloning of PCR-amplified DNA for highlevel expression of polypeptides. Using specifically designed primers for amplification and the pET-46 Ek/LIC Cloning Kit (Cat. No. 71335-3), inserts can be efficiently cloned without the need for restriction digestion or ligation. Fusion proteins contain an N-terminal cleavable His•Tag® sequence. Fusion to an optional C-terminal S•TagTM sequence can also be created for detection, purification and quantification of fusion proteins. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 Terminator Primer (Cat. No. 69337-3).

pET46 EK/LIC载体序列

ORIGIN

    1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg

   61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc

  121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg

  181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc

  241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt

  301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc

  361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta

  421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat

  481 ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt

  541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt

  601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc

  661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg

  721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc

  781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg

  841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca

  901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga

  961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct

 1021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg

 1081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca

 1141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata

 1201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct

 1261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact

 1321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa

 1381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc

 1441 atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg

 1501 atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac

 1561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag

 1621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg

 1681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca

 1741 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga

 1801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca

 1861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc

 1921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca

 1981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa

 2041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc

 2101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc

 2161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg

 2221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat

 2281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca

 2341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt

 2401 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac

 2461 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg

 2521 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg

 2581 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg

 2641 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg

 2701 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga

 2761 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg

 2821 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa

 2881 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt

 2941 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt

 3001 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct

 3061 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac

 3121 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag

 3181 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc

 3241 cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc

 3301 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc

 3361 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga

 3421 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt

 3481 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt

 3541 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg

 3601 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc

 3661 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat

 3721 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt

 3781 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc

 3841 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc

 3901 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag

 3961 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt

 4021 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat

 4081 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag

 4141 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac

 4201 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg

 4261 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg

 4321 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt

 4381 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc

 4441 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact

 4501 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg

 4561 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga

 4621 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc

 4681 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt

 4741 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg

 4801 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc

 4861 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag

 4921 aaataatttt gtttaacttt aagaaggaga tataccatgg cacatcacca ccaccatcac

 4981 gtggatgacg acgacaagag tccgggcttc tcctcaaatc tcgagtctgg taaagaaacc

 5041 gctgctgcga aatttgaacg ccagcacatg gactcgtcta ctagcgcagc ttaattaacc

 5101 taggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc

 5161 ttgaggggtt ttttgctgaa aggaggaact atatccggat

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