pET-42c(+)
pET-42c(+)
编号 | 载体名称 |
北京华越洋生物VECT5010 | pET-42c(+) |
pET42c载体基本信息
别名: | pET42c, pET 42c |
质粒类型: | 大肠杆菌蛋白表达 |
表达水平: | 高 |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 5931 bp |
5' 测序引物: | T7或pGEX-5‘ |
5' 测序引物序列: | T7: 5'-TAATACGACTCACTATAGGG-3'; pGEX-5': 5'-GGGCTGGCAAGCCACGTTTGGTG-3' |
载体标签: | N-GST, N-His, N-S, C-His, N-Thrombin, N-Factor Xa |
载体抗性: | Kanamycin |
备注: | Encodes GST fusion tag; Nterm thrombin cleavage site; Nterm Factor Xa cleavage site; a,b,c vary by MCS. |
稳定性: | 瞬时表达 Transient |
组成型: | 组成型 Constitutive |
病毒/非病毒: | 非病毒 |
pET42c载体质粒图谱和多克隆位点信息
Feature Name | Start | End | |
T7_terminator | 120 | 1 | |
T7_Terminal_primer | 69 | 87 | |
S15 | 352 | 308 | |
6xHIS | 412 | 395 | |
GST (variant) | 1093 | 443 | |
T7_transl_en_RBS | 1118 | 1102 | |
lacO | 1163 | 1136 | |
T7_promoter | 1181 | 1163 | |
tet (300 - 563) | 1217 | 1480 | |
pBRrevBam_primer | 1288 | 1269 | |
lacI | 1563 | 2654 | |
ROP | 3226 | 3417 | |
pGEX_3_primer | 3433 | 3411 | |
pBR322_origin | 4451 | 3832 | |
KanR2 | 4557 | 5372 |
ORF | Start | End | |
ORF frame 3 | 1093 | 143 | |
ORF frame 2 | 1094 | 1822 | |
ORF frame 3 | 1695 | 2654 | |
ORF frame 3 | 4557 | 5372 |
Enzyme Name | Cut |
XhoI | 174 |
NotI | 182 |
EagI | 182 |
HindIII | 189 |
SalI | 195 |
SacI | 206 |
PstI | 214 |
AscI | 216 |
StuI | 226 |
EcoRI | 235 |
BamHI | 241 |
EcoRV | 251 |
NcoI | 256 |
AgeI | 290 |
KpnI | 297 |
BglII | 300 |
SacII | 391 |
SpeI | 422 |
MscI | 885 |
NdeI | 1092 |
XbaI | 1130 |
ApaI | 2133 |
HpaI | 2428 |
NruI | 4645 |
SmaI | 4862 |
XmaI | 4860 |
pET42c载体简介
The pET-42 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 terminator primer (cat. no. 69337-3). Vector encoded sequence can be completely removed when cloning into the PshAI site (as shown below) and then cleaving the GST fusion protein with Factor Xa.
pET42c载体序列
ORIGIN
1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
121 TGTTTAGCAG CCTAGGTATT AATCAATTAG TGGTGGTGGT GGTGGTGGTG GTGCTCGAGT
181 GCGGCCGCAA GCTTGTCGAC GGAGCTCGCC TGCAGGCGCG CCAAGGCCTG TACAGAATTC
241 GGATCCCCGA TATCGCCATG GACCCGCGTC CCTCAATACC GGAGCCACCA CCGGTACCCA
301 GATCTGGGCT GTCCATGTGC TGGCGTTCGA ATTTAGCAGC AGCGGTTTCT TTCATACCAA
361 TTGCAGTACT ACCGCGTGGC ACCAGACCCG CGGAGTGATG GTGATGGTGA TGACCAGAAC
421 CACTAGTTGA ACCATCCGAT TTTGGAGGAT GGTCGCCACC ACCAAACGTG GCTTGCCAGC
481 CCTGCAAAGG CCATGCTATA TACTTGCTGG ATTTCAAGTA CTTATCAATT TGTGGGATAG
541 CTTCAATACG TTTTTTAAAA CAAACTAATT TTGGGAACGC ATCCAGGCAC ATTGGGTCCA
601 TGTATAAAAC AACATCAAGA GCGTCATACA ACATGAAGTC AGGATGGGTT ACATGATCAC
661 CATTTAAATA TGTTTTATGA CATAAACGAT CTTCGAACAT TTTCAGCATT TCAGGTAGCT
721 TGCTAAGAAA ATCAACTTTG AGAGTTTCAA AGTCTTTACT ATATGCAATT CTCGAAACAC
781 CGTATCTAAT ATCCAAAACC GCTCCTTCAA GCATTGAAAT CTCTGCACGC TCTTTTGGAC
841 AACCACCCAA CATGTTGTGC TTGTCAGCTA TATAACGTAT GATGGCCATA GACTGTGTTA
901 ATTTAACATC ACCATCAATA TAATAAGGAA GATTGGGAAA CTCCAAACCC AATTCAAACT
961 TTTTGTTTCG CCATTTATCA CCTTCATCGC GCTCATACAA ATGCTCTTCA TATTTTTCTT
1021 CAAGATATTC CAAAAGAAGT CGAGTGGGTT GCACAAGGCC CTTAATTTTC CAATAACCTA
1081 GTATAGGGGA CATATGTATA TCTCCTTCTT AAAGTTAAAC AAAATTATTT CTAGAGGGGA
1141 ATTGTTATCC GCTCACAATT CCCCTATAGT GAGTCGTATT AATTTCGCGG GATCGAGATC
1201 GATCTCGATC CTCTACGCCG GACGCATCGT GGCCGGCATC ACCGGCGCCA CAGGTGCGGT
1261 TGCTGGCGCC TATATCGCCG ACATCACCGA TGGGGAAGAT CGGGCTCGCC ACTTCGGGCT
1321 CATGAGCGCT TGTTTCGGCG TGGGTATGGT GGCAGGCCCC GTGGCCGGGG GACTGTTGGG
1381 CGCCATCTCC TTGCATGCAC CATTCCTTGC GGCGGCGGTG CTCAACGGCC TCAACCTACT
1441 ACTGGGCTGC TTCCTAATGC AGGAGTCGCA TAAGGGAGAG CGTCGAGATC CCGGACACCA
1501 TCGAATGGCG CAAAACCTTT CGCGGTATGG CATGATAGCG CCCGGAAGAG AGTCAATTCA
1561 GGGTGGTGAA TGTGAAACCA GTAACGTTAT ACGATGTCGC AGAGTATGCC GGTGTCTCTT
1621 ATCAGACCGT TTCCCGCGTG GTGAACCAGG CCAGCCACGT TTCTGCGAAA ACGCGGGAAA
1681 AAGTGGAAGC GGCGATGGCG GAGCTGAATT ACATTCCCAA CCGCGTGGCA CAACAACTGG
1741 CGGGCAAACA GTCGTTGCTG ATTGGCGTTG CCACCTCCAG TCTGGCCCTG CACGCGCCGT
1801 CGCAAATTGT CGCGGCGATT AAATCTCGCG CCGATCAACT GGGTGCCAGC GTGGTGGTGT
1861 CGATGGTAGA ACGAAGCGGC GTCGAAGCCT GTAAAGCGGC GGTGCACAAT CTTCTCGCGC
1921 AACGCGTCAG TGGGCTGATC ATTAACTATC CGCTGGATGA CCAGGATGCC ATTGCTGTGG
1981 AAGCTGCCTG CACTAATGTT CCGGCGTTAT TTCTTGATGT CTCTGACCAG ACACCCATCA
2041 ACAGTATTAT TTTCTCCCAT GAAGACGGTA CGCGACTGGG CGTGGAGCAT CTGGTCGCAT
2101 TGGGTCACCA GCAAATCGCG CTGTTAGCGG GCCCATTAAG TTCTGTCTCG GCGCGTCTGC
2161 GTCTGGCTGG CTGGCATAAA TATCTCACTC GCAATCAAAT TCAGCCGATA GCGGAACGGG
2221 AAGGCGACTG GAGTGCCATG TCCGGTTTTC AACAAACCAT GCAAATGCTG AATGAGGGCA
2281 TCGTTCCCAC TGCGATGCTG GTTGCCAACG ATCAGATGGC GCTGGGCGCA ATGCGCGCCA
2341 TTACCGAGTC CGGGCTGCGC GTTGGTGCGG ACATCTCGGT AGTGGGATAC GACGATACCG
2401 AAGACAGCTC ATGTTATATC CCGCCGTTAA CCACCATCAA ACAGGATTTT CGCCTGCTGG
2461 GGCAAACCAG CGTGGACCGC TTGCTGCAAC TCTCTCAGGG CCAGGCGGTG AAGGGCAATC
2521 AGCTGTTGCC CGTCTCACTG GTGAAAAGAA AAACCACCCT GGCGCCCAAT ACGCAAACCG
2581 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG
2641 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTAAGTTAGC TCACTCATTA GGCACCGGGA
2701 TCTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG GGCGCGGGGC
2761 ATGACTAGCA TGATCGTGCT CCTGTCGTTG AGGACCCGGC TAGGCTGGCG GGGTTGCCTT
2821 ACTGGTTAGC AGAATGAATC ACCGATACGC GAGCGAACGT GAAGCGACTG CTGCTGCAAA
2881 ACGTCTGCGA CCTGAGCAAC AACATGAATG GTCTTCGGTT TCCGTGTTTC GTAAAGTCTG
2941 GAAACGCGGA AGTCAGCGCC CTGCACCATT ATGTTCCGGA TCTGCATCGC AGGATGCTGC
3001 TGGCTACCCT GTGGAACACC TACATCTGTA TTAACGAAGC GCTGGCATTG ACCCTGAGTG
3061 ATTTTTCTCT GGTCCCGCCG CATCCATACC GCCAGTTGTT TACCCTCACA ACGTTCCAGT
3121 AACCGGGCAT GTTCATCATC AGTAACCCGT ATCGTGAGCA TCCTCTCTCG TTTCATCGGT
3181 ATCATTACCC CCATGAACAG AAATCCCCCT TACACGGAGG CATCAGTGAC CAAACAGGAA
3241 AAAACCGCCC TTAACATGGC CCGCTTTATC AGAAGCCAGA CATTAACGCT TCTGGAGAAA
3301 CTCAACGAGC TGGACGCGGA TGAACAGGCA GACATCTGTG AATCGCTTCA CGACCACGCT
3361 GATGAGCTTT ACCGCAGCTG CCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC
3421 ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC
3481 CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC CCAGTCACGT
3541 AGCGATAGCG GAGTGTATAC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG
3601 TGCACCATAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG
3661 GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC
3721 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG
3781 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT
3841 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
3901 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
3961 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
4021 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
4081 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
4141 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
4201 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
4261 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
4321 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
4381 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
4441 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
4501 TTTGGTCATG AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA
4561 GCCATATTCA ACGGGAAACG TCTTGCTCTA GGCCGCGATT AAATTCCAAC ATGGATGCTG
4621 ATTTATATGG GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC
4681 GATTGTATGG GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG
4741 CCAATGATGT TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC
4801 CGACCATCAA GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC
4861 CCGGGAAAAC AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG
4921 ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA
4981 ACAGCGATCG CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG
5041 ATGCGAGTGA TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA
5101 TGCATAAACT TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG
5161 ATAACCTTAT TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA
5221 TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT
5281 CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC
5341 AGTTTCATTT GATGCTCGAT GAGTTTTTCT AAGAATTAAT TCATGAGCGG ATACATATTT
5401 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
5461 CCTGAAATTG TAAACGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC
5521 TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC
5581 GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC
5641 TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA
5701 CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG
5761 AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG
5821 AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC
5881 ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT CCCATTCGCC A
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其他大肠杆菌表达载体：
