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1

pACYCDuet-1

pACYCDuet-1

pACYCDuet-1

 

编号

名称

北京华越洋VECT-400

pACYCDuet-1

 

pACYCDuet1载体基本信息

别名:

pACYCDuet1, pACYCDuet 1

质粒类型:

大肠杆菌蛋白双基因表达载体,有两个多克隆位点

表达水平:

克隆方法:

多克隆位点,限制性内切酶

载体大小:

4008 bp

5' 测序引物:

T7

5' 测序引物序列:  

T7: 5'-TAATACGACTCACTATAGGG-3';

3' 测序引物:

T7t

3' 测序引物序列:  

T7t: 5'-TGCTAGTTATTGCTCAGCGG-3'

载体标签:

N-His, C-S

载体抗性:

氯霉素

备注:

pACYCDuet-1 is designed for the   coexpression of two target genes. pACYCDuet-1 carries the P15A replicon, lacI   gene and chloramphenicol resistance gene.

稳定性:

瞬时表达 Transient

组成型:

组成型   Constitutive

病毒/非病毒:

非病毒

 

pACYCDuet1载体质粒图谱和多克隆位点信息

 

pACYCDuet1载体简介

pACYCDue-1 is designed for the coexpression of two target genes. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7 promoter/lac operator and ribosome binding site (rbs). The vector also carries the P15A replicon, lacI gene and chloramphenicol resistance gene. This vector can be used in combination with pACYCDuet™-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of up to 4 target genes. Genes inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer (Cat. No. 71178-3) and DuetDOWN1 Primer (Cat. No. 71179- 3). Genes inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3).

 

pACYCDuet1载体序列

ORIGIN

    1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag

   61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag

  121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag

  181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt

  241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata

  301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt

  361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg

  421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg

  481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg

  541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt

  601 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat

  661 ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct

  721 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt

  781 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc

  841 accttgtcgc cttgcgtata atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc

  901 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa

  961 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca

 1021 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat

 1081 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc

 1141 accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca

 1201 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag

 1261 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc

 1321 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt

 1381 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt

 1441 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat

 1501 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat

 1561 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct

 1621 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc

 1681 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc

 1741 cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc

 1801 agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt

 1861 gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc

 1921 ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac

 1981 ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga

 2041 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag

 2101 ataccaggcg tttcccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt

 2161 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc

 2221 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg

 2281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc

 2341 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt

 2401 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt

 2461 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt

 2521 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc

 2581 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca

 2641 gccccatacg atataagttg taattctcat gttagtcatg ccccgcgccc accggaagga

 2701 gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag

 2761 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg

 2821 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca

 2881 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc

 2941 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt

 3001 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta

 3061 ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg

 3121 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca

 3181 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa

 3241 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac

 3301 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc

 3361 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga

 3421 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt

 3481 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg

 3541 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca

 3601 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac

 3661 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt

 3721 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa

 3781 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg

 3841 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc

 3901 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc

 3961 tctcccttat gcgactcctg cattaggaaa ttaatacgac tcactata

//

 

其他大肠杆菌表达载体:

pKD13

PinPoint Xa-1

pKD4

pTf16

pTWIN2

pTYB11

pKJE7

pET-17b

pTrcHis2 A

pBad/gIII B

pGEX-6P-2

pG-KJE8

pET-46 EK/LIC

pMal-p2G

pGEX-2TK

pGEX-4T-3

pET-49b(+)

pEZZ18

pMal-c2X

pET300/NT-DEST

pBAD-TOPO

pMal-p2X

pET-33b(+)

pRSET-CFP

pET-23(+)

pMal-c4X

pET-24c(+)

pTrcHis2 B

pET-11b(+)

pET-41a(+)

pET-24(+)

pET-44c(+)

pQE-82L

pGEX-4T-1

pMal-p4X

pET-43.1c(+)

pQE-9

pALEX a,b,c

pBad/gIII C

pET-27b(+)

pQE-70

pKD20

pET102/D-TOPO

pET-21a(+)

pET-16b

pRSET B

pACYCDuet-1

pET-20b(+)

pET-21d(+)

pCOLADuet-1

pCYB1

pET-14b

pRSET-EmGFP

pET-42b(+)

pGEX-5X-2

pET-26b(+)

pET301/CT-DEST

pGEX-6P-1

pET-28c(+)

pET-50b(+)

pGro7

pRSET A

pET-32a(+)

pMal-c2G

pET-11a(+)

pRSET C

ptdTomato

pQE-30

pET-12b(+)

pET-42c(+)

pET-43.1b(+)

pET-41c(+)

pET-23b(+)

pET-39b(+)

pBV220

pET-41c(+)

pBad/His C

pET-32b(+)

pCold IV

pETDuet-1

pBAD/Thio-TOPO

pET-29c(+)

pE-SUMO

pTYB12

pCold-ProS2

pET-24b(+)

pACYC184

pEGM-7ZF(+)

pCold III

pBAD33

pBad/Myc-His A

pSP73

pCold-GST

pAmCyan

PinPoint Xa-2

pTXB1

pET-52b(+)

pColdS-SUMO

pTWIN1

pBad/gIII A

pET-23d(+)

pCold II

pET-24a(+)

pkk223-3

pET-29a(+)

pBAD102/D-TOPO

pET-28a(+)

pBAD18

pET-30 EK/LIC

pBad/Myc-His C

pProEX HTc

pET-44 EK/LIC

pET-32 Xa/LIC

pBad/His B

pMal-p2E

pTrcHis B

pET-40b(+)

pET-23a(+)

pQE-60

pGEX-3X

pET-45b(+)

pET-12c(+)

pQE-32

pTrc99a

pMal-c2E

pET-11c(+)

pBad24

pACYC177

pGEX-5X-1

pQE-80L

pET-12a(+)

pMXB10

pGEX-6P-3

pQE-31

pET-23c(+)

pGEX-KG

pTrcHis A

pET-51b(+)

pBad/His A

pTrcHis C

pGEX-2T

pET-47b(+)

pBad/Myc-His B

pET-3a(+)

pEcoli-6xHN-GFPuv

pET-22b(+)

pBAD202/D-TOPO

pGEX-5X-3

pBR322

pET-19b

pCold I

pET-32 EK/LIC

pET-28b(+)

pET-21b(+)

pCold TF

pET-30 Xa/LIC

pGEX-4T-2

pET-25b(+)

pQE-81L

pET-29b(+)

pET-31b(+)

pET-42a(+)

pQE-16

pGEM-T

pET-43.1 EK/LIC

pET-44a(+)

pQE-40

pBlueScript SK(+)

pMAL-c5x

pTrcHis2 C

pET-48b(+)

pET-SUMO

pkk232-8

pET302/NT-His

pET-21b(+)

pCDFDuet-1

pET-5a(+)

pGFPuv

pET-15b

pBluescript II SK(+)

pET-5b(+)

pBad43

pET-21c(+)

pProEX HTb

pSP64

pG-Tf2

pET-24d(+)

pKD46

PinPoint Xa-3

pSE380

pET-30a(+)

pBluescript II KS(-)

pMAL-p5e

pMAL-p5x

pET-43.1a(+)

pEGM-11ZF(+)

pTYB2

pET-30b(+)

pET-37b(+)

pDsRed-Express2

pKD3

pProEX HTa

pET303/CT-His

pBV221

pSUMO

pTYB1

pRSET-BFP

pET-11d(+)

pET-3b(+)

pET-41 EK/LIC

pET-32c(+)

pET-44b(+)

pRSFDuet-1

pET-30c(+)

pET-His

pET-41b(+)