pET-11c(+)
pET-11c(+)
编号 | 载体名称 |
北京华越洋生物VECT5150 | pET-11c(+) |
pet11c载体基本信息
别名: | pET-11c, pet 11c |
质粒类型: | 大肠杆菌表达载体 |
表达水平: | 高 |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 5672bp |
5' 测序引物: | T7 |
5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
载体标签: | T7 tag (N-端） |
载体抗性: | Ampicillin |
备注: | Approximate size is 5675 bp. Hosts: E.coli. Related vectors: pBR322. |
稳定性: | 瞬时表达 Transient |
组成型: | 组成型 Constitutive |
病毒/非病毒: | 非病毒 |
pet11c载体质粒图谱和多克隆位点信息
Feature Name | Start | End | |
T7_terminator | 316 | 188 | |
T7_Terminal_primer | 256 | 274 | |
T7_leader | 358 | 326 | |
Xpress_fwd_primer | 359 | 341 | |
T7_transl_en_RBS | 383 | 367 | |
lacO | 428 | 401 | |
T7_promoter | 446 | 428 | |
pBRrevBam_primer | 549 | 530 | |
lacI | 824 | 1915 | |
tet (576 - 1190) | 1974 | 2588 | |
ROP | 3227 | 3418 | |
pGEX_3_primer | 3434 | 3412 | |
Ampicillin | 5464 | 4604 | |
AmpR_promoter | 5534 | 5506 |
ORF | Start | End | |
ORF frame 1 | 583 | 1083 | |
ORF frame 2 | 956 | 1915 | |
ORF frame 1 | 2393 | 1698 | |
ORF frame 3 | 1971 | 2588 | |
ORF frame 2 | 5464 | 4604 |
Enzyme Name | Cut |
ClaI | 24 |
HindIII | 29 |
BamHI | 319 |
NheI | 350 |
NdeI | 357 |
XbaI | 395 |
BglII | 461 |
BclI | 1197 |
ApaI | 1394 |
HpaI | 1689 |
EagI | 2251 |
NruI | 2286 |
MscI | 2758 |
PstI | 4922 |
AatII | 5599 |
EcoRI | 5670 |
pet11c载体简介
pET-11a-d 载体具有N端T7标签和 BamH I 多克隆克隆位点。这些载体是很多pET家族载体的前身。pET-21a-d(+)系列载体分别对应于 pET-11a-d 载体，但是包含有几个额外的特征序列。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。
The pET-11a-d vectors carry an N-terminal T7•Tag® sequence and BamH I cloning site. Thesevectors are the precursors to many pET family vectors; the pET-21a-d(+) series corresponds topET-11a-d but incorporates several additional features. Unique sites are shown on the circle map.Note that the sequence is numbered by the pBR322 convention, so the T7 expression region isreversed on the circular map.
pet11c载体序列
ORIGIN
1 TTCTCATGTT TGACAGCTTA TCATCGATAA GCTTTAATGC GGTAGTTTAT CACAGTTAAA
61 TTGCTAACGC AGTCAGGCAC CGTGTATGAA ATCTAACAAT GCGCTCATCG TCATCCTCGG
121 CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG CCGGTACTGC CGGGCCTCTT
181 GCGGGATATC CGGATATAGT TCCTCCTTTC AGCAAAAAAC CCCTCAAGAC CCGTTTAGAG
241 GCCCCAAGGG GTTATGCTAG TTATTGCTCA GCGGTGGCAG CAGCCAACTC AGCTTCCTTT
301 CGGGCTTTGT TAGCAGCCGG ATCCGACCCA TTTGCTGTCC ACCAGTCATG CTAGCCATAT
361 GTATATCTCC TTCTTAAAGT TAAACAAAAT TATTTCTAGA GGGGAATTGT TATCCGCTCA
421 CAATTCCCCT ATAGTGAGTC GTATTAATTT CGCGGGATCG AGATCTCGAT CCTCTACGCC
481 GGACGCATCG TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC
541 GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC TTGTTTCGGC
601 GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG GCGCCATCTC CTTGCATGCA
661 CCATTCCTTG CGGCGGCGGT GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG
721 CAGGAGTCGC ATAAGGGAGA GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT
781 TCGCGGTATG GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC
841 AGTAACGTTA TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT
901 GGTGAACCAG GCCAGCCACG TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC
961 GGAGCTGAAT TACATTCCCA ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT
1021 GATTGGCGTT GCCACCTCCA GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT
1081 TAAATCTCGC GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG
1141 CGTCGAAGCC TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT
1201 CATTAACTAT CCGCTGGATG ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT
1261 TCCGGCGTTA TTTCTTGATG TCTCTGACCA GACACCCATC AACAGTATTA TTTTCTCCCA
1321 TGAAGACGGT ACGCGACTGG GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC
1381 GCTGTTAGCG GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA
1441 ATATCTCACT CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT GGAGTGCCAT
1501 GTCCGGTTTT CAACAAACCA TGCAAATGCT GAATGAGGGC ATCGTTCCCA CTGCGATGCT
1561 GGTTGCCAAC GATCAGATGG CGCTGGGCGC AATGCGCGCC ATTACCGAGT CCGGGCTGCG
1621 CGTTGGTGCG GATATCTCGG TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT
1681 CCCGCCGTTA ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG
1741 CTTGCTGCAA CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC CCGTCTCACT
1801 GGTGAAAAGA AAAACCACCC TGGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC
1861 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA
1921 ACGCAATTAA TGTAAGTTAG CTCACTCATT AGGCACCGGG ATCTCGACCG ATGCCCTTGA
1981 GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC
2041 TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG CTCTGGGTCA
2101 TTTTCGGCGA GGACCGCTTT CGCTGGAGCG CGACGATGAT CGGCCTGTCG CTTGCGGTAT
2161 TCGGAATCTT GCACGCCCTC GCTCAAGCCT TCGTCACTGG TCCCGCCACC AAACGTTTCG
2221 GCGAGAAGCA GGCCATTATC GCCGGCATGG CGGCCGACGC GCTGGGCTAC GTCTTGCTGG
2281 CGTTCGCGAC GCGAGGCTGG ATGGCCTTCC CCATTATGAT TCTTCTCGCT TCCGGCGGCA
2341 TCGGGATGCC CGCGTTGCAG GCCATGCTGT CCAGGCAGGT AGATGACGAC CATCAGGGAC
2401 AGCTTCAAGG ATCGCTCGCG GCTCTTACCA GCCTAACTTC GATCACTGGA CCGCTGATCG
2461 TCACGGCGAT TTATGCCGCC TCGGCGAGCA CATGGAACGG GTTGGCATGG ATTGTAGGCG
2521 CCGCCCTATA CCTTGTCTGC CTCCCCGCGT TGCGTCGCGG TGCATGGAGC CGGGCCACCT
2581 CGACCTGAAT GGAAGCCGGC GGCACCTCGC TAACGGATTC ACCACTCCAA GAATTGGAGC
2641 CAATCAATTC TTGCGGAGAA CTGTGAATGC GCAAACCAAC CCTTGGCAGA ACATATCCAT
2701 CGCGTCCGCC ATCTCCAGCA GCCGCACGCG GCGCATCTCG GGCAGCGTTG GGTCCTGGCC
2761 ACGGGTGCGC ATGATCGTGC TCCTGTCGTT GAGGACCCGG CTAGGCTGGC GGGGTTGCCT
2821 TACTGGTTAG CAGAATGAAT CACCGATACG CGAGCGAACG TGAAGCGACT GCTGCTGCAA
2881 AACGTCTGCG ACCTGAGCAA CAACATGAAT GGTCTTCGGT TTCCGTGTTT CGTAAAGTCT
2941 GGAAACGCGG AAGTCAGCGC CCTGCACCAT TATGTTCCGG ATCTGCATCG CAGGATGCTG
3001 CTGGCTACCC TGTGGAACAC CTACATCTGT ATTAACGAAG CGCTGGCATT GACCCTGAGT
3061 GATTTTTCTC TGGTCCCGCC GCATCCATAC CGCCAGTTGT TTACCCTCAC AACGTTCCAG
3121 TAACCGGGCA TGTTCATCAT CAGTAACCCG TATCGTGAGC ATCCTCTCTC GTTTCATCGG
3181 TATCATTACC CCCATGAACA GAAATCCCCC TTACACGGAG GCATCAGTGA CCAAACAGGA
3241 AAAAACCGCC CTTAACATGG CCCGCTTTAT CAGAAGCCAG ACATTAACGC TTCTGGAGAA
3301 ACTCAACGAG CTGGACGCGG ATGAACAGGC AGACATCTGT GAATCGCTTC ACGACCACGC
3361 TGATGAGCTT TACCGCAGCT GCCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA
3421 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC
3481 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC GCAGCCATGA CCCAGTCACG
3541 TAGCGATAGC GGAGTGTATA CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA
3601 GTGCACCATT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC
3661 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG
3721 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA
3781 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG
3841 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA
3901 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG
3961 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG
4021 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC
4081 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
4141 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA
4201 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT
4261 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG
4321 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG
4381 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
4441 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT
4501 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT
4561 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA
4621 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG
4681 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC
4741 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG
4801 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC
4861 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTG
4921 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC
4981 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC
5041 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC
5101 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT
5161 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA
5221 CACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT
5281 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA
5341 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA
5401 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC
5461 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG
5521 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC
5581 GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA
5641 GGCGTATCAC GAGGCCCTTT CGTCTTCAAG AA
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其他大肠杆菌表达载体：
