pET-44 EK/LIC

pET-44 EK/LIC

pET44 EK/LIC载体基本信息

pET44 EK/LIC载体质粒图谱和多克隆位点信息

pET44 EK/LIC载体简介

The pET-44 Ek/LIC Vector is prepared for rapid, directional cloning of PCR-amplified DNA for high-level expression of polypeptides fused with N-terminal His•Tag®,Nus•Tag™ and S•Tag™ sequences. Using specifically designed primers for amplification and the pET-44 Ek/LIC Vector Kit (Cat. No. 71144-3), inserts can be efficiently cloned without the need for restriction digestion or ligation. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the COLIDOWN Primer (Cat. No. 70845-3). Vector encoded sequences can be completely removed when cloning into the Ek/LIC site (as shown below left) by cleaving the fusion protein with enterokinase.

pET44 EK/LIC载体序列

ORIGIN

    1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA

   61 GGGGTTATGC TAGTTATTGC TCAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA

  121 AAAGAGTTTG TAGAAACGCA AAAAGGCCAT CCGTCAGGAT GGCCTTCTGC TTAATTTGAT

  181 GCCTGGCAGT TTATGGCGGG CGTCCTGCCC GCCACCCTCC GGGCCGTTGC TTCGCAACGT

  241 TCAAATCCGC TCCCGGCGGA TTTGTCCTAC TCAGGAGAGC GTTCACCGAC AAACAACAGA

  301 TAAAACGAAA GGCCCAGTCT TTCGACTGAG CCTTTCGTTT TATTTGATGC CTGGCAGTTC

  361 CCTACTCTCG CATGGGGAGA CCCCACACTA CCATCGGCGC TACGGCGTTT CACTTCTGAG

  421 TTCGGCATGG GGTCAGGTGG GACCACCGCG CTACTGCCGC CAGGCAAATT CTGTTTTATC

  481 AGCCTAGGAA CGCCCAACTT AATTAACATT AGTGGTGGTG GTGGTGGTGC TCGAGATCCT

  541 CTGGGTCTTC AGGAGCGAGT TCTGGCTGGC TTGCACGTGT ATACAGCTGT GCGGCCGCAA

  601 GCTTCGAACG CGTATCGATG GTACCGTCGA CGTCCTGCAG GCGCGCCTGT ACAGAATTCG

  661 GATCCACGAG CTCAGATATC GTTGAGGAGA AGCCCGGACT CTTGTCGTCG TCATCAATCG

  721 TACCAGAACC CGCGCTGCCC CGGGGGACCA GACCAGTTGG CGGTGGCGAG TCCATGTGCT

  781 GGCGTTCAAA TTTCGCAGCA GCGGTTTCTT TACCCGCGGA GTGATGGTGA TGGTGATGAC

  841 CAGAACCACT AGTCGCTTCG TCACCGAACC AGCAAATATT ACGGGCAGCC ATAATCAGTG

  901 CTCCGGCTTT TTCGTCGGTC AACCCTTCGA TATCAGCCAG ATCATCAATG CCCTGTTCGG

  961 CGAGATCTTC CAGCGTACAA ACGCCACGGG CGGCCAGTTT GAATGCCAAA TCACGATCTA

 1021 CCCCTTCAAG GTTCAGCAGA TCGTCAGCCG GTTTGTTATC ACCGAGGCTT TCTTCCTGGG

 1081 CCTGTGCAAT GGTGGCCAGT GCATTTTTAG CACGCTCGCG CAGTGCTTCA ACGGTCGGCT

 1141 CATCAAGGCC TTCGATTTCC AACAGCTCTT TCATCGGCAC ATAGGCCAAT TCTTCCAGCG

 1201 TCGAGAAGCC TTCTTCTACC AGAACAGTCG CGAAGTCTTC GTCGATGTCG AGATATTTGG

 1261 TGAAGGTGTC GATCGCTGCG TGCGCTTCCG CCTGATGCTT AGCTTGCAGG TCGTCAACGG

 1321 TCATCACGTT GAGTTCCCAA CCGCTCAGTT GCGAAGCCAG ACGCACGTTC TGACCGTTAC

 1381 GGCCAATCGC CTGCGCCAGA TTACCGGCTT CAACGGCGAT GTCCATGGTG TGTTTATCTT

 1441 CATCCACCAC GATAGAAGCA ACGTCTGCCG GTGCCATTGC GTTAATCACG AACTGCGCCG

 1501 GGTTATCATC CCACAGGACG ATATCGATAC GCTCGCCACC CAGTTCAGTA GACACCGCCT

 1561 GAACACGCGC GCCACGCATA CCTACGCAAG CACCTACCGG ATCGATACGT TTATCGTTGG

 1621 TTTTCACCGC GATTTTCGCA CGAGAACCCG GATCGCGAGC CGCTGCTTTA ATTTCAATCA

 1681 CTTCTTCGCC GATTTCTGGC ACTTCAATAC GGAACAGTTC GATCAGCATT TCCGGCTTGG

 1741 AACGAGTGAC GAACAGTTGC GCGCCACGCG CTTCCGGGCG AACGGAATAG AGCACGCCAC

 1801 GAACGCGGTC GCCAGGGCGG AAGTTTTCAC GCGGCAGCAT ATCTTCGCGC AGGATCACGG

 1861 CTTCAGCGTT GTTGCCCAGA TCCAGAGAGA TGTTGTCGCG GTTTACTTTT TTCACCACGC

 1921 CGGTGATGAT TTCACCTTCG TGTTCACGGA ACTGATCAAC CACCATCGCA CGTTCGGCTT

 1981 CACGCACTTT CTGCACGATA ACCTGTTTTG CCGTCTGGGT AGTGATACGG TCAAAGGTAA

 2041 CAGACTCAAT CTGATCTTCA ACGTAATCGC CCAGGTTCAG GCTTTCATCT TCATAACGTG

 2101 CGGCTTCAAG GGTGATTTCC TTGGTCGGCT GGGTGACTTC ATCAACAACT AACCAGCGAC

 2161 GGAAAGTGTC AAAATCACCG CTTTTGCGAT CGATCTGTAC GCGGACGTCG ATCTCTTGTT

 2221 CATATTTTTT CTTTGTTGCT GTCGCCAGCG CGCTTTCCAA TGCTTCGAAA ATCTTCTCGC

 2281 GAGGTAGCGC CTTTTCATTG GATACGGCTT CAACTACAGC CAAAATTTCT TTGTTCATAC

 2341 TAGAATGGTG ATGGTGGTGA TGGCTGCTGC CCATATGTAT ATCTCCTTCT TAAAGTTAAA

 2401 CAAAATTATT TCTAGAGGGG AATTGTTATC CGCTCACAAT TCCCCTATAG TGAGTCGTAT

 2461 TAATTTCGCG GGATCGAGAT CGATCTCGAT CCTCTACGCC GGACGCATCG TGGCCGGCAT

 2521 CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC GACATCACCG ATGGGGAAGA

 2581 TCGGGCTCGC CACTTCGGGC TCATGAGCGC TTGTTTCGGC GTGGGTATGG TGGCAGGCCC

 2641 CGTGGCCGGG GGACTGTTGG GCGCCATCTC CTTGCATGCA CCATTCCTTG CGGCGGCGGT

 2701 GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG CAGGAGTCGC ATAAGGGAGA

 2761 GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT TCGCGGTATG GCATGATAGC

 2821 GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC AGTAACGTTA TACGATGTCG

 2881 CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT GGTGAACCAG GCCAGCCACG

 2941 TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC GGAGCTGAAT TACATTCCCA

 3001 ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT GCCACCTCCA

 3061 GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC GCCGATCAAC

 3121 TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG CGTCGAAGCC TGTAAAGCGG

 3181 CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT CATTAACTAT CCGCTGGATG

 3241 ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT TCCGGCGTTA TTTCTTGATG

 3301 TCTCTGACCA GACACCCATC AACAGTATTA TTTTCTCCCA TGAAGACGGT ACGCGACTGG

 3361 GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG GGCCCATTAA

 3421 GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA ATATCTCACT CGCAATCAAA

 3481 TTCAGCCGAT AGCGGAACGG GAAGGCGACT GGAGTGCCAT GTCCGGTTTT CAACAAACCA

 3541 TGCAAATGCT GAATGAGGGC ATCGTTCCCA CTGCGATGCT GGTTGCCAAC GATCAGATGG

 3601 CGCTGGGCGC AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG GACATCTCGG

 3661 TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA ACCACCATCA

 3721 AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG CTTGCTGCAA CTCTCTCAGG

 3781 GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC CCGTCTCACT GGTGAAAAGA AAAACCACCC

 3841 TGGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG

 3901 CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTAAGTTAG

 3961 CTCACTCATT AGGCACCGGG ATCTCGACCG ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC

 4021 TCCTTCCGGT GGGCGCGGGG CATGACTAGC ATGATCGTGC TCCTGTCGTT GAGGACCCGG

 4081 CTAGGCTGGC GGGGTTGCCT TACTGGTTAG CAGAATGAAT CACCGATACG CGAGCGAACG

 4141 TGAAGCGACT GCTGCTGCAA AACGTCTGCG ACCTGAGCAA CAACATGAAT GGTCTTCGGT

 4201 TTCCGTGTTT CGTAAAGTCT GGAAACGCGG AAGTCAGCGC CCTGCACCAT TATGTTCCGG

 4261 ATCTGCATCG CAGGATGCTG CTGGCTACCC TGTGGAACAC CTACATCTGT ATTAACGAAG

 4321 CGCTGGCATT GACCCTGAGT GATTTTTCTC TGGTCCCGCC GCATCCATAC CGCCAGTTGT

 4381 TTACCCTCAC AACGTTCCAG TAACCGGGCA TGTTCATCAT CAGTAACCCG TATCGTGAGC

 4441 ATCCTCTCTC GTTTCATCGG TATCATTACC CCCATGAACA GAAATCCCCC TTACACGGAG

 4501 GCATCAGTGA CCAAACAGGA AAAAACCGCC CTTAACATGG CCCGCTTTAT CAGAAGCCAG

 4561 ACATTAACGC TTCTGGAGAA ACTCAACGAG CTGGACGCGG ATGAACAGGC AGACATCTGT

 4621 GAATCGCTTC ACGACCACGC TGATGAGCTT TACCGCAGCT GCCTCGCGCG TTTCGGTGAT

 4681 GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG

 4741 GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC

 4801 GCAGCCATGA CCCAGTCACG TAGCGATAGC GGAGTGTATA CTGGCTTAAC TATGCGGCAT

 4861 CAGAGCAGAT TGTACTGAGA GTGCACCATA TATGCGGTGT GAAATACCGC ACAGATGCGT

 4921 AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC

 4981 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC

 5041 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA

 5101 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA

 5161 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC

 5221 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA

 5281 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA

 5341 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA

 5401 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA

 5461 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG

 5521 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG

 5581 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG

 5641 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG

 5701 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA

 5761 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GACCTATTTG TTTATTTTTC TAAATACATT

 5821 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATCA TGATTGAAAA

 5881 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT

 5941 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG

 6001 TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT

 6061 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG

 6121 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG

 6181 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA

 6241 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG

 6301 ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA

 6361 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC

 6421 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT

 6481 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA

 6541 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG

 6601 CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA

 6661 GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG

 6721 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT

 6781 TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT

 6841 AATCTCATGC CATCGTGCCG CCAGAAATTG TAAACGTTAA TATTTTGTTA AAATTCGCGT

 6901 TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT

 6961 ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC

 7021 CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG

 7081 GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC

 7141 TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG

 7201 TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG

 7261 CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT

 7321 CCCATTCGCC A

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其他大肠杆菌表达载体：