넳 넲

pQE-60

编号	载体名称
北京华越洋生物VECT4120	pQE-60

pQE60载体基本信息

别名:	pQE60, pQE 60
质粒类型:	大肠杆菌蛋白表达载体
表达水平:	低拷贝
启动子:	T5
克隆方法:	多克隆位点，限制性内切酶
载体大小:	3431 bp
5' 测序引物及序列:	pQE30-F: AGCGGATAACAATTTCACACAG
3' 测序引物及序列:	pQE30-R: TTCTGAGGTCATTACTGGATC
载体标签:	C-His
载体抗性:	Ampicillin
备注:	蛋白质表达时使用的配套表达菌株为M15[pREP4].
稳定性:	瞬时表达 Transient
组成型:	组成型 Constitutive
病毒/非病毒:	非病毒

pQE60载体质粒图谱和多克隆位点信息

pQE60载体序列

ORIGIN

1 CTCGAGAAAT CATAAAAAAT TTATTTGCTT TGTGAGCGGA TAACAATTAT AATAGATTCA

61 ATTGTGAGCG GATAACAATT TCACACAGAA TTCATTAAAG AGGAGAAATT AACCATGGGA

121 GGATCCAGAT CTCATCACCA TCACCATCAC TAAGCTTAAT TAGCTGAGCT TGGACTCCTG

181 TTGATAGATC CAGTAATGAC CTCAGAACTC CATCTGGATT TGTTCAGAAC GCTCGGTTGC

241 CGCCGGGCGT TTTTTATTGG TGAGAATCCA AGCTAGCTTG GCGAGATTTT CAGGAGCTAA

301 GGAAGCTAAA ATGGAGAAAA AAATCACTGG ATATACCACC GTTGATATAT CCCAATGGCA

361 TCGTAAAGAA CATTTTGAGG CATTTCAGTC AGTTGCTCAA TGTACCTATA ACCAGACCGT

421 TCAGCTGGAT ATTACGGCCT TTTTAAAGAC CGTAAAGAAA AATAAGCACA AGTTTTATCC

481 GGCCTTTATT CACATTCTTG CCCGCCTGAT GAATGCTCAT CCGGAATTTC GTATGGCAAT

541 GAAAGACGGT GAGCTGGTGA TATGGGATAG TGTTCACCCT TGTTACACCG TTTTCCATGA

601 GCAAACTGAA ACGTTTTCAT CGCTCTGGAG TGAATACCAC GACGATTTCC GGCAGTTTCT

661 ACACATATAT TCGCAAGATG TGGCGTGTTA CGGTGAAAAC CTGGCCTATT TCCCTAAAGG

721 GTTTATTGAG AATATGTTTT TCGTCTCAGC CAATCCCTGG GTGAGTTTCA CCAGTTTTGA

781 TTTAAACGTG GCCAATATGG ACAACTTCTT CGCCCCCGTT TTCACCATGC ATGGGCAAAT

841 ATTATACGCA AGGCGACAAG GTGCTGATGC CGCTGGCGAT TCAGGTTCAT CATGCCGTCT

901 GTGATGGCTT CCATGTCGGC AGAATGCTTA ATGAATTACA ACAGTACTGC GATGAGTGGC

961 AGGGCGGGGC GTAATTTTTT TAAGGCAGTT ATTGGTGCCC TTAAACGCCT GGGGTAATGA

1021 CTCTCTAGCT TGAGGCATCA AATAAAACGA AAGGCTCAGT CGAAAGACTG GGCCTTTCGT

1081 TTTATCTGTT GTTTGTCGGT GAACGCTCTC CTGAGTAGGA CAAATCCGCC GCTCTAGAGC

1141 TGCCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG

1201 GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG

1261 GGTGTTGGCG GGTGTCGGGG CGCAGCCATG ACCCAGTCAC GTAGCGATAG CGGAGTGTAT

1321 ACTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG

1381 AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCTCTTCC GCTTCCTCGC

1441 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG

1501 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG

1561 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC

1621 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG

1681 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA

1741 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC

1801 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG

1861 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT

1921 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA

1981 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA

2041 CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG

2101 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA

2161 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG

2221 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA

2281 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA

2341 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG

2401 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA

2461 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC

2521 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC

2581 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA

2641 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC

2701 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT

2761 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA

2821 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG

2881 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG

2941 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC

3001 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT

3061 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT

3121 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG

3181 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC

3241 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA

3301 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG

3361 TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT

3421 TTCGTCTTCA C

pQE-60其他大肠杆菌表达载体：

pKD13	PinPoint Xa-1	pKD4	pTf16
pTWIN2	pTYB11	pKJE7	pET-17b
pTrcHis2 A	pBad/gIII B	pGEX-6P-2	pG-KJE8
pET-46 EK/LIC	pMal-p2G	pGEX-2TK	pGEX-4T-3
pET-49b(+)	pEZZ18	pMal-c2X	pET300/NT-DEST
pBAD-TOPO	pMal-p2X	pET-33b(+)	pRSET-CFP
pET-23(+)	pMal-c4X	pET-24c(+)	pTrcHis2 B
pET-11b(+)	pET-41a(+)	pET-24(+)	pET-44c(+)
pQE-82L	pGEX-4T-1	pMal-p4X	pET-43.1c(+)
pQE-9	pALEX a,b,c	pBad/gIII C	pET-27b(+)
pQE-70	pKD20	pET102/D-TOPO	pET-21a(+)
pET-16b	pRSET B	pACYCDuet-1	pET-20b(+)
pET-21d(+)	pCOLADuet-1	pCYB1	pET-14b
pRSET-EmGFP	pET-42b(+)	pGEX-5X-2	pET-26b(+)
pET301/CT-DEST	pGEX-6P-1	pET-28c(+)	pET-50b(+)
pGro7	pRSET A	pET-32a(+)	pMal-c2G
pET-11a(+)	pRSET C	ptdTomato	pQE-30
pET-12b(+)	pET-42c(+)	pET-43.1b(+)	pET-41c(+)
pET-23b(+)	pET-39b(+)	pBV220	pET-41c(+)
pBad/His C	pET-32b(+)	pCold IV	pETDuet-1
pBAD/Thio-TOPO	pET-29c(+)	pE-SUMO	pTYB12
pCold-ProS2	pET-24b(+)	pACYC184	pEGM-7ZF(+)
pCold III	pBAD33	pBad/Myc-His A	pSP73
pCold-GST	pAmCyan	PinPoint Xa-2	pTXB1
pET-52b(+)	pColdS-SUMO	pTWIN1	pBad/gIII A
pET-23d(+)	pCold II	pET-24a(+)	pkk223-3
pET-29a(+)	pBAD102/D-TOPO	pET-28a(+)	pBAD18
pET-30 EK/LIC	pBad/Myc-His C	pProEX HTc	pET-44 EK/LIC
pET-32 Xa/LIC	pBad/His B	pMal-p2E	pTrcHis B
pET-40b(+)	pET-23a(+)	pQE-60	pGEX-3X
pET-45b(+)	pET-12c(+)	pQE-32	pTrc99a
pMal-c2E	pET-11c(+)	pBad24	pACYC177
pGEX-5X-1	pQE-80L	pET-12a(+)	pMXB10
pGEX-6P-3	pQE-31	pET-23c(+)	pGEX-KG
pTrcHis A	pET-51b(+)	pBad/His A	pTrcHis C
pGEX-2T	pET-47b(+)	pBad/Myc-His B	pET-3a(+)
pEcoli-6xHN-GFPuv	pET-22b(+)	pBAD202/D-TOPO	pGEX-5X-3
pBR322	pET-19b	pCold I	pET-32 EK/LIC
pET-28b(+)	pET-21b(+)	pCold TF	pET-30 Xa/LIC
pGEX-4T-2	pET-25b(+)	pQE-81L	pET-29b(+)
pET-31b(+)	pET-42a(+)	pQE-16	pGEM-T
pET-43.1 EK/LIC	pET-44a(+)	pQE-40	pBlueScript SK(+)
pMAL-c5x	pTrcHis2 C	pET-48b(+)	pET-SUMO
pkk232-8	pET302/NT-His	pET-21b(+)	pCDFDuet-1
pET-5a(+)	pGFPuv	pET-15b	pBluescript II SK(+)
pET-5b(+)	pBad43	pET-21c(+)	pProEX HTb
pSP64	pG-Tf2	pET-24d(+)	pKD46
PinPoint Xa-3	pSE380	pET-30a(+)	pBluescript II KS(-)
pMAL-p5e	pMAL-p5x	pET-43.1a(+)	pEGM-11ZF(+)
pTYB2	pET-30b(+)	pET-37b(+)	pDsRed-Express2
pKD3	pProEX HTa	pET303/CT-His	pBV221
pSUMO	pTYB1	pRSET-BFP	pET-11d(+)
pET-3b(+)	pET-41 EK/LIC	pET-32c(+)	pET-44b(+)
pRSFDuet-1	pET-30c(+)	pET-His	pET-41b(+)