pTWIN2

pTWIN2载体基本信息

pTWIN2载体质粒图谱和多克隆位点信息

pTWIN2载体简介

pTWIN2 is an E. coli expression vector which can be used with the IMPACT? Kit (NEB #E6901) (1). A polylinker in the vector is designed for the in-frame fusion of a target gene between the modified Ssp DnaB (2) and Mth RIR1 inteins (3). The presence of the chitin binding domain from Bacillus circulans (4,5) facilitates purification. pTWIN vectors are designed for protein purification or for the isolation of proteins with an N-terminal cysteine and/or a C-terminal thioester (1). The double-stranded vector is 7192 base pairs in length.

pTWIN2载体序列

ORIGIN

        1 aactacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt

       61 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata

      121 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt

      181 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc

      241 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat

      301 ccttgagagt tttcgccccg aagaacgttc tccaatgatg agcactttta aagttctgct

      361 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca

      421 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg

      481 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa

      541 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg

      601 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga

      661 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg

      721 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt

      781 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg

      841 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc

      901 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca

      961 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc

     1021 atatatactt tagattgatt taccccggtt gataatcaga aaagccccaa aaacaggaag

     1081 attgtataag caaatattta aattgtaaac gttaatattt tgttaaaatt cgcgttaaat

     1141 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa

     1201 tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa gagtccacta

     1261 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca

     1321 ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat

     1381 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg

     1441 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc

     1501 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtaaaag

     1561 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc

     1621 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt

     1681 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt

     1741 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat

     1801 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc

     1861 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa

     1921 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg

     1981 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag

     2041 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag

     2101 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa

     2161 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt

     2221 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg

     2281 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc

     2341 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac

     2401 cgagcgcagc gagtcagtga gcgaggaagc tatggtgcac tctcagtaca atctgctctg

     2461 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc

     2521 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc

     2581 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc

     2641 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gcagcgattc

     2701 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt

     2761 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc

     2821 ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat

     2881 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa

     2941 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcc

     3001 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt

     3061 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat

     3121 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc

     3181 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt

     3241 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa

     3301 ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat

     3361 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg

     3421 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt

     3481 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca

     3541 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg

     3601 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac

     3661 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg

     3721 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac

     3781 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga

     3841 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta

     3901 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt

     3961 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg

     4021 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt

     4081 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt

     4141 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag

     4201 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc

     4261 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca

     4321 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt

     4381 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg

     4441 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac

     4501 cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac

     4561 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg

     4621 aatggtgcat gccggcatgc cgccctttcg tcttcaagaa ttaattccca attccccagg

     4681 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg

     4741 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag

     4801 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa ttaattcccc

     4861 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt

     4921 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg

     4981 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gaattaattc

     5041 cccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt

     5101 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt

     5161 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggaattaa

     5221 ttccccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc

     5281 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac

     5341 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggaat

     5401 taattcccca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt

     5461 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg

     5521 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg

     5581 aattggggat cggaattaat tcccggttta aaccggggat ctcgatcccg cgaaattaat

     5641 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt

     5701 taactttaag aaggagatat acatatgaaa atcgaagaag gtaaactgac aaatcctggt

     5761 gtatccgctt ggcaggtcaa cacagcttat actgcgggac aattggtcac atataacggc

     5821 aagacgtata aatgtttgca gccccacacc tccttggcag gatgggaacc atccaacgtt

     5881 cctgccttgt ggcagcttca aaacaacggt aacaacggtc tcgaactgcg cgagtccgga

     5941 gctatctctg gcgatagtct gatcagcctg gctagcacag gaaaaagagt ttctattaaa

     6001 gatttgttag atgaaaaaga ttttgaaata tgggcaatta atgaacagac gatgaagcta

     6061 gaatcagcta aagttagtcg tgtattttgt actggcaaaa agctagttta tattctaaaa

     6121 actcgactag gtagaactat caaggcaaca gcaaatcata gatttttaac tattgatggt

     6181 tggaaaagat tagatgagct atctttaaaa gagcatattg ctctaccccg taaactagaa

     6241 agctcctctt tacaattgtc accagaaata gaaaagttgt ctcagagtga tatttactgg

     6301 gactccatcg tttctattac ggagactgga gtcgaagagg tttttgattt gactgtgcca

     6361 ggaccacata actttgtcgc gaatgacatc attgtacaca acggaagagc catgggcggc

     6421 cgcgaattcc tcgagggctc ttcctgcgta tccggtgaca ccattgtaat gactagtggc

     6481 ggtccgcgca ctgtggctga actggagggc aaaccgttca ccgcactgat tcgcggctct

     6541 ggctacccat gcccctcagg tttcttccgc acctgtgaac gtgacgtata tgatctgcgt

     6601 acacgtgagg gtcattgctt acgtttgacc catgatcacc gtgttctggt gatggatggt

     6661 ggcctggaat ggcgtgccgc gggtgaactg gaacgcggcg accgcctggt gatggatgat

     6721 gcagctggcg agtttccggc actggcaacc ttccgtggcc tgcgtggcgc tggccgccag

     6781 gatgtttatg acgctactgt ttacggtgct agcgcattca ctgctaatgg cttcattgta

     6841 cacgcatgcg gcgagcagcc cgggaccggt ctgaactcag gcctcacgac aaatcctggt

     6901 gtatccgctt ggcaggtcaa cacagcttat actgcgggac aattggtcac atataacggc

     6961 aagacgtata aatgtttgca gccccacacc tccttggcag gatgggaacc atccaacgtt

     7021 cctgccttgt ggcagcttca atgactgcag gaaggggatc cggctgctaa caaagcccga

     7081 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc

     7141 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg at

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