pET-24c(+)
pET-24c(+)
编号 | 名称 |
北京华越洋VECT-620 | pET-24c(+) |
pET24c载体基本信息
别名: | pET24c, pet 24c |
质粒类型: | 大肠杆菌蛋白表达 |
表达水平: | 高 |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 5308 bp |
5' 测序引物: | T7 |
5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
载体标签: | N-T7, C-His |
载体抗性: | Kanamycin |
备注: | Same as pET21a,b,c,d(+) but kanamycin resistance; a,b,c,d vary by MCS; The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. |
稳定性: | 瞬时表达 Transient |
组成型: | 组成型 Constitutive |
病毒/非病毒: | 非病毒 |
pET24c载体质粒图谱和多克隆位点信息
Feature Name | Start | End |
T7_terminator | 129 | 1 |
T7_Terminal_primer | 69 | 87 |
T7_leader | 237 | 205 |
Xpress_fwd_primer | 238 | 220 |
T7_transl_en_RBS | 262 | 246 |
lacO | 307 | 280 |
T7_promoter | 325 | 307 |
tet (300 - 563) | 357 | 620 |
pBRrevBam_primer | 428 | 409 |
lacI | 703 | 1794 |
tet (576 - 851) | 1853 | 2128 |
ROP | 2603 | 2794 |
pGEX_3_primer | 2810 | 2788 |
pBR322_origin | 3828 | 3209 |
KanR2 | 3934 | 4749 |
ORF | Start | End |
ORF frame 3 | 462 | 962 |
ORF frame 1 | 835 | 1794 |
ORF frame 1 | 3934 | 4749 |
Enzyme Name | Cut |
XhoI | 158 |
NotI | 166 |
EagI | 166 |
HindIII | 173 |
SalI | 179 |
SacI | 190 |
EcoRI | 192 |
BamHI | 198 |
NheI | 229 |
NdeI | 236 |
XbaI | 274 |
BglII | 340 |
BclI | 1076 |
ApaI | 1273 |
EcoRV | 1512 |
HpaI | 1568 |
FspI | 2144 |
NruI | 4022 |
ClaI | 4056 |
XmaI | 4237 |
SmaI | 4239 |
pET24c载体简介
The pET-24a-d(+) vectors carry an N-terminal T7•Tag® sequence plus an optional C-terminal His•Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs. ampicillin resistance). Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, singlestranded sequencing should be performed using the T7 terminator primer.
pET24c载体序列
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg
241 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac
301 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg
361 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
421 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
481 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
541 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
601 aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt
661 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca
721 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg
781 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
841 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg
901 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt
961 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc
1021 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
1081 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt
1141 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat
1201 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg
1261 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa
1321 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg
1381 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg
1441 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc
1501 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc
1561 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
1621 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
1681 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc
1741 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
1801 cgcaattaat gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag
1861 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
1921 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat
1981 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt
2041 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg
2101 cgagaagcag gccattatcg ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct
2161 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc
2221 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac
2281 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg
2341 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac
2401 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat
2461 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt
2521 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa
2581 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg
2641 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga
2701 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct
2761 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
2821 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
2881 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
2941 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa
3001 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
3061 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
3121 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
3181 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
3241 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
3301 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
3361 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
3421 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
3481 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
3541 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
3601 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
3661 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
3721 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
3781 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
3841 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaac aataaaactg
3901 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct
3961 tgctctaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
4021 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
4081 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
4141 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
4201 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta
4261 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
4321 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
4381 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
4441 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
4501 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
4561 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
4621 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
4681 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
4741 tttttctaag aattaattca tgagcggata catatttgaa tgtatttaga aaaataaaca
4801 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gaaattgtaa acgttaatat
4861 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
4921 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
4981 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
5041 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
5101 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
5161 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
5221 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
5281 gccgctacag ggcgcgtccc attcgcca
//
其他大肠杆菌表达载体：
