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pSE380

编号	载体名称
北京华越洋生物VECT5310	pSE380

pSE380载体基本信息

载体名称:	pSE380
质粒类型:	大肠杆菌表达载体
高拷贝/低拷贝:	高拷贝
启动子:	trc
克隆方法:	多克隆位点，限制性内切酶
载体大小:	4476 bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	无标签
载体抗性:	氨苄青霉素（Ampicillin）
筛选标记:	--
备注:	--
稳定性:	--
组成型:	--
病毒/非病毒:	--

pSE380载体质粒图谱和多克隆位点信息

pSE380载体简介

E.coli NM522, E.coli INValphaF' 相关载体: pTrc99A, pSE280, pSE420

pSE380载体序列

ORIGIN

1 AATTCTCATG TTTGACAGCT TATCATCGAC TGCACGGTGC ACCAATGCTT CTGGCGTCAG

61 GCAGCCATCG GAAGCTGTGG TATGGCTGTG CAGGTCGTAA ATCACTGCAT AATTCGTGTC

121 GCTCAAGGCG CACTCCCGTT CTGGATAATG TTTTTTGCGC CGACATCATA ACGGTTCTGG

181 CAAATATTCT GAAATGAGCT GTTGACAATT AATCATCCGG CTCGTATAAT GTGTGGAATT

241 GTGAGCGGAT AACAATTTCA CACAGGAAAC AGACCATGGC TGGTGACCAC GTCGTGGAAT

301 GCCTTCGAAT TCAGCACCTG CACATGGGAC GTCGACCTGA GGTAATTATA ACCCGGGCCC

361 TATATATGGA TCCAATTGCA ATGATCATCA TGACAGATCT GCGCGCGATC GATATCAGCG

421 CTTTAAATTT GCGCATGCTA GCTATAGTTC TAGAGGTACC GGTTGTTAAC GTTAGCCGGC

481 TACGTATACT CCGGAATATT AATAGGCCTA GGATGCATAT GGCGGCCGCC TGCAGCTGGC

541 GCCATCGATA CGCGTACGTC GCGACCGCGG ACATGTACAG AGCTCGAGAA GTACTAGTGG

601 CCACGTGGGC CGTGCACCTT AAGCTTGGCT GTTTTGGCGG ATGAGAGAAG ATTTTCAGCC

661 TGATACAGAT TAAATCAGAA CGCAGAAGCG GTCTGATAAA ACAGAATTTG CCTGGCGGCA

721 GTAGCGCGGT GGTCCCACCT GACCCCATGC CGAACTCAGA AGTGAAACGC CGTAGCGCCG

781 ATGGTAGTGT GGGGTCTCCC CATGCGAGAG TAGGGAACTG CCAGGCATCA AATAAAACGA

841 AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC

901 CTGAGTAGGA CAAATCCGCC GGGAGCGGAT TTGAACGTTG CGAAGCAACG GCCCGGAGGG

961 TGGCGGGCAG GACGCCCGCC ATAAACTGCC AGGCATCAAA TTAAGCAGAA GGCCATCCTG

1021 ACGGATGGCC TTTTTGCGTT TCTACAAACT CTTTTTGTTT ATTTTTCTAA ATACATTCAA

1081 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA

1141 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC

1201 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG

1261 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC

1321 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT

1381 TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG

1441 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG

1501 AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA

1561 CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC

1621 GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA

1681 CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC

1741 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC

1801 TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG

1861 GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA

1921 TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG

1981 GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA

2041 TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC

2101 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA

2161 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA

2221 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC

2281 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT

2341 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC

2401 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC

2461 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA

2521 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG

2581 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG

2641 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT

2701 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT

2761 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC

2821 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT

2881 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG

2941 CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA

3001 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC

3061 GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC

3121 GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG

3181 GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCAGATCAA

3241 TTCGCGCGCG AAGGCGAAGC GGCATGCATT TACGTTGACA CCATCGAATG GCGCAAAACC

3301 TTTCGCGGTA TGGCATGATA GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT GAATGTGAAA

3361 CCAGTAACGT TATACGATGT CGCAGAGTAT GCCGGTGTCT CTTATCAGAC CGTTTCCCGC

3421 GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA AGCGGCGATG

3481 GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA ACAGTCGTTG

3541 CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT TGTCGCGGCG

3601 ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT AGAACGAAGC

3661 GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT CAGTGGGCTG

3721 ATCATTAACT ATCCGCTGGA TGACCAGGAT GCCATTGCTG TGGAAGCTGC CTGCACTAAT

3781 GTTCCGGCGT TATTTCTTGA TGTCTCTGAC CAGACACCCA TCAACAGTAT TATTTTCTCC

3841 CATGAAGACG GTACGCGACT GGGCGTGGAG CATCTGGTCG CATTGGGTCA CCAGCAAATC

3901 GCGCTGTTAG CGGGCCCATT AAGTTCTGTC TCGGCGCGTC TGCGTCTGGC TGGCTGGCAT

3961 AAATATCTCA CTCGCAATCA AATTCAGCCG ATAGCGGAAC GGGAAGGCGA CTGGAGTGCC

4021 ATGTCCGGTT TTCAACAAAC CATGCAAATG CTGAATGAGG GCATCGTTCC CACTGCGATG

4081 CTGGTTGCCA ACGATCAGAT GGCGCTGGGC GCAATGCGCG CCATTACCGA GTCCGGGCTG

4141 CGCGTTGGTG CGGATATCTC GGTAGTGGGA TACGACGATA CCGAAGACAG CTCATGTTAT

4201 ATCCCGCCGT CAACCACCAT CAAACAGGAT TTTCGCCTGC TGGGGCAAAC CAGCGTGGAC

4261 CGCTTGCTGC AACTCTCTCA GGGCCAGGCG GTGAAGGGCA ATCAGCTGTT GCCCGTCTCA

4321 CTGGTGAAAA GAAAAACCAC CCTGGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG

4381 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG

4441 CAACGCAATT AATGTGAGTT AGCGCGAATT GATCTT

其他大肠杆菌表达载体：

pKD13	PinPoint Xa-1	pKD4	pTf16
pTWIN2	pTYB11	pKJE7	pET-17b
pTrcHis2 A	pBad/gIII B	pGEX-6P-2	pG-KJE8
pET-46 EK/LIC	pMal-p2G	pGEX-2TK	pGEX-4T-3
pET-49b(+)	pEZZ18	pMal-c2X	pET300/NT-DEST
pBAD-TOPO	pMal-p2X	pET-33b(+)	pRSET-CFP
pET-23(+)	pMal-c4X	pET-24c(+)	pTrcHis2 B
pET-11b(+)	pET-41a(+)	pET-24(+)	pET-44c(+)
pQE-82L	pGEX-4T-1	pMal-p4X	pET-43.1c(+)
pQE-9	pALEX a,b,c	pBad/gIII C	pET-27b(+)
pQE-70	pKD20	pET102/D-TOPO	pET-21a(+)
pET-16b	pRSET B	pACYCDuet-1	pET-20b(+)
pET-21d(+)	pCOLADuet-1	pCYB1	pET-14b
pRSET-EmGFP	pET-42b(+)	pGEX-5X-2	pET-26b(+)
pET301/CT-DEST	pGEX-6P-1	pET-28c(+)	pET-50b(+)
pGro7	pRSET A	pET-32a(+)	pMal-c2G
pET-11a(+)	pRSET C	ptdTomato	pQE-30
pET-12b(+)	pET-42c(+)	pET-43.1b(+)	pET-41c(+)
pET-23b(+)	pET-39b(+)	pBV220	pET-41c(+)
pBad/His C	pET-32b(+)	pCold IV	pETDuet-1
pBAD/Thio-TOPO	pET-29c(+)	pE-SUMO	pTYB12
pCold-ProS2	pET-24b(+)	pACYC184	pEGM-7ZF(+)
pCold III	pBAD33	pBad/Myc-His A	pSP73
pCold-GST	pAmCyan	PinPoint Xa-2	pTXB1
pET-52b(+)	pColdS-SUMO	pTWIN1	pBad/gIII A
pET-23d(+)	pCold II	pET-24a(+)	pkk223-3
pET-29a(+)	pBAD102/D-TOPO	pET-28a(+)	pBAD18
pET-30 EK/LIC	pBad/Myc-His C	pProEX HTc	pET-44 EK/LIC
pET-32 Xa/LIC	pBad/His B	pMal-p2E	pTrcHis B
pET-40b(+)	pET-23a(+)	pQE-60	pGEX-3X
pET-45b(+)	pET-12c(+)	pQE-32	pTrc99a
pMal-c2E	pET-11c(+)	pBad24	pACYC177
pGEX-5X-1	pQE-80L	pET-12a(+)	pMXB10
pGEX-6P-3	pQE-31	pET-23c(+)	pGEX-KG
pTrcHis A	pET-51b(+)	pBad/His A	pTrcHis C
pGEX-2T	pET-47b(+)	pBad/Myc-His B	pET-3a(+)
pEcoli-6xHN-GFPuv	pET-22b(+)	pBAD202/D-TOPO	pGEX-5X-3
pBR322	pET-19b	pCold I	pET-32 EK/LIC
pET-28b(+)	pET-21b(+)	pCold TF	pET-30 Xa/LIC
pGEX-4T-2	pET-25b(+)	pQE-81L	pET-29b(+)
pET-31b(+)	pET-42a(+)	pQE-16	pGEM-T
pET-43.1 EK/LIC	pET-44a(+)	pQE-40	pBlueScript SK(+)
pMAL-c5x	pTrcHis2 C	pET-48b(+)	pET-SUMO
pkk232-8	pET302/NT-His	pET-21b(+)	pCDFDuet-1
pET-5a(+)	pGFPuv	pET-15b	pBluescript II SK(+)
pET-5b(+)	pBad43	pET-21c(+)	pProEX HTb
pSP64	pG-Tf2	pET-24d(+)	pKD46
PinPoint Xa-3	pSE380	pET-30a(+)	pBluescript II KS(-)
pMAL-p5e	pMAL-p5x	pET-43.1a(+)	pEGM-11ZF(+)
pTYB2	pET-30b(+)	pET-37b(+)	pDsRed-Express2
pKD3	pProEX HTa	pET303/CT-His	pBV221
pSUMO	pTYB1	pRSET-BFP	pET-11d(+)
pET-3b(+)	pET-41 EK/LIC	pET-32c(+)	pET-44b(+)
pRSFDuet-1	pET-30c(+)	pET-His	pET-41b(+)