北京华越洋生物
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1

pSE380

pSE380

pSE380

 

编号

载体名称

北京华越洋生物VECT5310

pSE380

 

pSE380载体基本信息

载体名称:

pSE380

质粒类型:

大肠杆菌表达载体

高拷贝/低拷贝:

高拷贝

启动子:

trc

克隆方法:

多克隆位点,限制性内切酶

载体大小:

4476 bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

无标签

载体抗性:

氨苄青霉素(Ampicillin

筛选标记:

--

备注:

--

稳定性:

--

组成型:

--

病毒/非病毒:

--

 

pSE380载体质粒图谱和多克隆位点信息


 

pSE380载体简介

E.coli NM522, E.coli INValphaF' 相关载体: pTrc99A, pSE280, pSE420

 

pSE380载体序列

ORIGIN

    1 AATTCTCATG TTTGACAGCT TATCATCGAC TGCACGGTGC ACCAATGCTT CTGGCGTCAG

   61 GCAGCCATCG GAAGCTGTGG TATGGCTGTG CAGGTCGTAA ATCACTGCAT AATTCGTGTC

  121 GCTCAAGGCG CACTCCCGTT CTGGATAATG TTTTTTGCGC CGACATCATA ACGGTTCTGG

  181 CAAATATTCT GAAATGAGCT GTTGACAATT AATCATCCGG CTCGTATAAT GTGTGGAATT

  241 GTGAGCGGAT AACAATTTCA CACAGGAAAC AGACCATGGC TGGTGACCAC GTCGTGGAAT

  301 GCCTTCGAAT TCAGCACCTG CACATGGGAC GTCGACCTGA GGTAATTATA ACCCGGGCCC

  361 TATATATGGA TCCAATTGCA ATGATCATCA TGACAGATCT GCGCGCGATC GATATCAGCG

  421 CTTTAAATTT GCGCATGCTA GCTATAGTTC TAGAGGTACC GGTTGTTAAC GTTAGCCGGC

  481 TACGTATACT CCGGAATATT AATAGGCCTA GGATGCATAT GGCGGCCGCC TGCAGCTGGC

  541 GCCATCGATA CGCGTACGTC GCGACCGCGG ACATGTACAG AGCTCGAGAA GTACTAGTGG

  601 CCACGTGGGC CGTGCACCTT AAGCTTGGCT GTTTTGGCGG ATGAGAGAAG ATTTTCAGCC

  661 TGATACAGAT TAAATCAGAA CGCAGAAGCG GTCTGATAAA ACAGAATTTG CCTGGCGGCA

  721 GTAGCGCGGT GGTCCCACCT GACCCCATGC CGAACTCAGA AGTGAAACGC CGTAGCGCCG

  781 ATGGTAGTGT GGGGTCTCCC CATGCGAGAG TAGGGAACTG CCAGGCATCA AATAAAACGA

  841 AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC

  901 CTGAGTAGGA CAAATCCGCC GGGAGCGGAT TTGAACGTTG CGAAGCAACG GCCCGGAGGG

  961 TGGCGGGCAG GACGCCCGCC ATAAACTGCC AGGCATCAAA TTAAGCAGAA GGCCATCCTG

 1021 ACGGATGGCC TTTTTGCGTT TCTACAAACT CTTTTTGTTT ATTTTTCTAA ATACATTCAA

 1081 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA

 1141 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC

 1201 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG

 1261 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC

 1321 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT

 1381 TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG

 1441 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG

 1501 AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA

 1561 CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC

 1621 GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA

 1681 CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC

 1741 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC

 1801 TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG

 1861 GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA

 1921 TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG

 1981 GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA

 2041 TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC

 2101 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA

 2161 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA

 2221 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC

 2281 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT

 2341 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC

 2401 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC

 2461 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA

 2521 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG

 2581 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG

 2641 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT

 2701 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT

 2761 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC

 2821 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT

 2881 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG

 2941 CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA

 3001 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC

 3061 GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC

 3121 GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG

 3181 GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCAGATCAA

 3241 TTCGCGCGCG AAGGCGAAGC GGCATGCATT TACGTTGACA CCATCGAATG GCGCAAAACC

 3301 TTTCGCGGTA TGGCATGATA GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT GAATGTGAAA

 3361 CCAGTAACGT TATACGATGT CGCAGAGTAT GCCGGTGTCT CTTATCAGAC CGTTTCCCGC

 3421 GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA AGCGGCGATG

 3481 GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA ACAGTCGTTG

 3541 CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT TGTCGCGGCG

 3601 ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT AGAACGAAGC

 3661 GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT CAGTGGGCTG

 3721 ATCATTAACT ATCCGCTGGA TGACCAGGAT GCCATTGCTG TGGAAGCTGC CTGCACTAAT

 3781 GTTCCGGCGT TATTTCTTGA TGTCTCTGAC CAGACACCCA TCAACAGTAT TATTTTCTCC

 3841 CATGAAGACG GTACGCGACT GGGCGTGGAG CATCTGGTCG CATTGGGTCA CCAGCAAATC

 3901 GCGCTGTTAG CGGGCCCATT AAGTTCTGTC TCGGCGCGTC TGCGTCTGGC TGGCTGGCAT

 3961 AAATATCTCA CTCGCAATCA AATTCAGCCG ATAGCGGAAC GGGAAGGCGA CTGGAGTGCC

 4021 ATGTCCGGTT TTCAACAAAC CATGCAAATG CTGAATGAGG GCATCGTTCC CACTGCGATG

 4081 CTGGTTGCCA ACGATCAGAT GGCGCTGGGC GCAATGCGCG CCATTACCGA GTCCGGGCTG

 4141 CGCGTTGGTG CGGATATCTC GGTAGTGGGA TACGACGATA CCGAAGACAG CTCATGTTAT

 4201 ATCCCGCCGT CAACCACCAT CAAACAGGAT TTTCGCCTGC TGGGGCAAAC CAGCGTGGAC

 4261 CGCTTGCTGC AACTCTCTCA GGGCCAGGCG GTGAAGGGCA ATCAGCTGTT GCCCGTCTCA

 4321 CTGGTGAAAA GAAAAACCAC CCTGGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG

 4381 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG

 4441 CAACGCAATT AATGTGAGTT AGCGCGAATT GATCTT

//

 

其他大肠杆菌表达载体:

pKD13

PinPoint Xa-1

pKD4

pTf16

pTWIN2

pTYB11

pKJE7

pET-17b

pTrcHis2 A

pBad/gIII B

pGEX-6P-2

pG-KJE8

pET-46 EK/LIC

pMal-p2G

pGEX-2TK

pGEX-4T-3

pET-49b(+)

pEZZ18

pMal-c2X

pET300/NT-DEST

pBAD-TOPO

pMal-p2X

pET-33b(+)

pRSET-CFP

pET-23(+)

pMal-c4X

pET-24c(+)

pTrcHis2 B

pET-11b(+)

pET-41a(+)

pET-24(+)

pET-44c(+)

pQE-82L

pGEX-4T-1

pMal-p4X

pET-43.1c(+)

pQE-9

pALEX a,b,c

pBad/gIII C

pET-27b(+)

pQE-70

pKD20

pET102/D-TOPO

pET-21a(+)

pET-16b

pRSET B

pACYCDuet-1

pET-20b(+)

pET-21d(+)

pCOLADuet-1

pCYB1

pET-14b

pRSET-EmGFP

pET-42b(+)

pGEX-5X-2

pET-26b(+)

pET301/CT-DEST

pGEX-6P-1

pET-28c(+)

pET-50b(+)

pGro7

pRSET A

pET-32a(+)

pMal-c2G

pET-11a(+)

pRSET C

ptdTomato

pQE-30

pET-12b(+)

pET-42c(+)

pET-43.1b(+)

pET-41c(+)

pET-23b(+)

pET-39b(+)

pBV220

pET-41c(+)

pBad/His C

pET-32b(+)

pCold IV

pETDuet-1

pBAD/Thio-TOPO

pET-29c(+)

pE-SUMO

pTYB12

pCold-ProS2

pET-24b(+)

pACYC184

pEGM-7ZF(+)

pCold III

pBAD33

pBad/Myc-His A

pSP73

pCold-GST

pAmCyan

PinPoint Xa-2

pTXB1

pET-52b(+)

pColdS-SUMO

pTWIN1

pBad/gIII A

pET-23d(+)

pCold II

pET-24a(+)

pkk223-3

pET-29a(+)

pBAD102/D-TOPO

pET-28a(+)

pBAD18

pET-30 EK/LIC

pBad/Myc-His C

pProEX HTc

pET-44 EK/LIC

pET-32 Xa/LIC

pBad/His B

pMal-p2E

pTrcHis B

pET-40b(+)

pET-23a(+)

pQE-60

pGEX-3X

pET-45b(+)

pET-12c(+)

pQE-32

pTrc99a

pMal-c2E

pET-11c(+)

pBad24

pACYC177

pGEX-5X-1

pQE-80L

pET-12a(+)

pMXB10

pGEX-6P-3

pQE-31

pET-23c(+)

pGEX-KG

pTrcHis A

pET-51b(+)

pBad/His A

pTrcHis C

pGEX-2T

pET-47b(+)

pBad/Myc-His B

pET-3a(+)

pEcoli-6xHN-GFPuv

pET-22b(+)

pBAD202/D-TOPO

pGEX-5X-3

pBR322

pET-19b

pCold I

pET-32 EK/LIC

pET-28b(+)

pET-21b(+)

pCold TF

pET-30 Xa/LIC

pGEX-4T-2

pET-25b(+)

pQE-81L

pET-29b(+)

pET-31b(+)

pET-42a(+)

pQE-16

pGEM-T

pET-43.1 EK/LIC

pET-44a(+)

pQE-40

pBlueScript SK(+)

pMAL-c5x

pTrcHis2 C

pET-48b(+)

pET-SUMO

pkk232-8

pET302/NT-His

pET-21b(+)

pCDFDuet-1

pET-5a(+)

pGFPuv

pET-15b

pBluescript II SK(+)

pET-5b(+)

pBad43

pET-21c(+)

pProEX HTb

pSP64

pG-Tf2

pET-24d(+)

pKD46

PinPoint Xa-3

pSE380

pET-30a(+)

pBluescript II KS(-)

pMAL-p5e

pMAL-p5x

pET-43.1a(+)

pEGM-11ZF(+)

pTYB2

pET-30b(+)

pET-37b(+)

pDsRed-Express2

pKD3

pProEX HTa

pET303/CT-His

pBV221

pSUMO

pTYB1

pRSET-BFP

pET-11d(+)

pET-3b(+)

pET-41 EK/LIC

pET-32c(+)

pET-44b(+)

pRSFDuet-1

pET-30c(+)

pET-His

pET-41b(+)