pBAD18

pBAD18

pBAD18载体基本信息

pBAD18载体质粒图谱和多克隆位点信息

pBAD18载体简介

This vector is one of several tightly controlled expression vectors (ATCC#8739387402) regulated by the arabinose operon. Vectors differ in replicon, antibiotic resistance marker,  multiple cloning site and need for translation initiation sequences. Cultures should be grown in  minimal media for more reproducible induction of expression. Expression is induced in glycerolcontaining  media by the addition of arabinose.  Expression is repressed by addition of glucose or other catabolites. Cloned inserts must provide a translation  initiation sequence (ATG) and ribosome binding site for expression. Expression of cloned inserts in the two recommended hosts differ in terms of induction characteristics, especially when using low concentrations of inducer (arabinose) and growing in minimal media. The following primers can be used for sequencing of cloned inserts: 5 primer (278bp upstream of the NheI site):5 CTGTTTCTCCATACCCGTT 3and one of two 3 primers:3 primer 1 (219bp downstream of the HindIII site):5 CTCATCCGCCAAAACAG 33 primer 2 (1733bp downstream of  the HindIII site):5 GGCTGAAAATCTTCTCT3

pBAD18载体序列

ORIGIN

    1 ATCGATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC

   61 TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA

  121 TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA

  181 AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA

  241 GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG

  301 CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG

  361 CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG

  421 TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT

  481 TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC

  541 CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC

  601 GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA

  661 TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA

  721 TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA

  781 ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA

  841 TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC

  901 GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT

  961 TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG AAGAAACCAA TTGTCCATAT

 1021 TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA

 1081 ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT

 1141 AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA

 1201 CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG ATCCTACCTG ACGCTTTTTA

 1261 TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGCGAATT CGAGCTCGGT

 1321 ACCCGGGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC TTGGCTGTTT TGGCGGATGA

 1381 GAGAAGATTT TCAGCCTGAT ACAGATTAAA TCAGAACGCA GAAGCGGTCT GATAAAACAG

 1441 AATTTGCCTG GCGGCAGTAG CGCGGTGGTC CCACCTGACC CCATGCCGAA CTCAGAAGTG

 1501 AAACGCCGTA GCGCCGATGG TAGTGTGGGG TCTCCCCATG CGAGAGTAGG GAACTGCCAG

 1561 GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT

 1621 GTCGGTGAAC GCTCTCCTGA GTAGGACAAA TCCGCCGGGA GCGGATTTGA ACGTTGCGAA

 1681 GCAACGGCCC GGAGGGTGGC GGGCAGGACG CCCGCCATAA ACTGCCAGGC ATCAAATTAA

 1741 GCAGAAGGCC ATCCTGACGG ATGGCCTTTT TGCGTTTCTA CAAACTCTTT TGTTTATTTT

 1801 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT

 1861 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT

 1921 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG

 1981 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA

 2041 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC

 2101 TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC

 2161 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG

 2221 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA

 2281 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG

 2341 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG

 2401 ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG

 2461 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG

 2521 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG

 2581 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT

 2641 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC

 2701 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT

 2761 CATATATACT TTAGATTGAT TTACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG

 2821 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT

 2881 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG

 2941 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTTG

 3001 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG

 3061 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTT GAACAACACT CAACCCTATC

 3121 TCGGGCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT

 3181 GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGTT TACAATTTAA

 3241 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT

 3301 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT

 3361 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG

 3421 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA

 3481 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT

 3541 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA

 3601 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC

 3661 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT

 3721 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA

 3781 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG

 3841 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT

 3901 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT

 3961 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA

 4021 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC

 4081 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT

 4141 CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC

 4201 TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT

 4261 GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA

 4321 TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG

 4381 TCATCACCGA AACGCGCGAG GCAGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG

 4441 GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC

 4501 TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT

 4561 GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CTGCTCATGT TTGACAGCTT ATC

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