pACYC177

pACYC177

pACYC177载体基本信息

pACYC177载体质粒图谱和多克隆位点信息

pACYC177载体简介

pACYC177 is an E. coli plasmid cloning vector containing the p15A origin of replication (1-4). This allows pACYC177 to coexist in cells with plasmids of the ColE1 compatibility group (e.g., pBR322, pUC19). It is a low copy number vector, at about 15 copies per cell (5), but can be amplified with chloramphenicol or spectinomycin. Enzymes with unique restriction sites are shown in bold type and enzymes with two restriction sites are shown in regular type. Location of sites of all NEB restriction enzymes can be found on the NEB web site (choose Technical Reference > DNA Sequences and Maps). Restriction site coordinates refer to the position of the 5′-most base on the top strand in each recognition sequence.

pACYC177载体序列

ORIGIN

    1 GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT

   61 GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC

  121 AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA

  181 GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT

  241 CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT

  301 GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC

  361 CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG

  421 GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC

  481 GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG

  541 ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA

  601 CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA

  661 AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC

  721 AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCTTAAT AAGATGATCT

  781 TCTTGAGATC GTTTTGGTCT GCGCGTAATC TCTTGCTCTG AAAACGAAAA AACCGCCTTG

  841 CAGGGCGGTT TTTCGAAGGT TCTCTGAGCT ACCAACTCTT TGAACCGAGG TAACTGGCTT

  901 GGAGGAGCGC AGTCACCAAA ACTTGTCCTT TCAGTTTAGC CTTAACCGGC GCATGACTTC

  961 AAGACTAACT CCTCTAAATC AATTACCAGT GGCTGCTGCC AGTGGTGCTT TTGCATGTCT

 1021 TTCCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG ACTGAACGGG

 1081 GGGTTCGTGC ATACAGTCCA GCTTGGAGCG AACTGCCTAC CCGGAACTGA GTGTCAGGCG

 1141 TGGAATGAGA CAAACGCGGC CATAACAGCG GAATGACACC GGTAAACCGA AAGGCAGGAA

 1201 CAGGAGAGCG CACGAGGGAG CCGCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG

 1261 GGTTTCGCCA CCACTGATTT GAGCGTCAGA TTTCGTGATG CTTGTCAGGG GGGCGGAGCC

 1321 TATGGAAAAA CGGCTTTGCC GCGGCCCTCT CACTTCCCTG TTAAGTATCT TCCTGGCATC

 1381 TTCCAGGAAA TCTCCGCCCC GTTCGTAAGC CATTTCCGCT CGCCGCAGTC GAACGACCGA

 1441 GCGTAGCGAG TCAGTGAGCG AGGAAGCGGA ATATATCCTG TATCACATAT TCTGCTGACG

 1501 CACCGGTGCA GCCTTTTTTC TCCTGCCACA TGAAGCACTT CACTGACACC CTCATCAGTG

 1561 CCAACATAGT AAGCCAGTAT ACACTCCGCT AGCGCTGAGG TCTGCCTCGT GAAGAAGGTG

 1621 TTGCTGACTC ATACCAGGCC TGAATCGCCC CATCATCCAG CCAGAAAGTG AGGGAGCCAC

 1681 GGTTGATGAG AGCTTTGTTG TAGGTGGACC AGTTGGTGAT TTTGAACTTT TGCTTTGCCA

 1741 CGGAACGGTC TGCGTTGTCG GGAAGATGCG TGATCTGATC CTTCAACTCA GCAAAAGTTC

 1801 GATTTATTCA ACAAAGCCAC GTTGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA

 1861 AAAATATATC ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG

 1921 TTATGAGCCA TATTCAACGG GAAACGTCTT GCTCGAGGCC GCGATTAAAT TCCAACATGG

 1981 ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA

 2041 TCTATCGATT GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA

 2101 GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC

 2161 CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG

 2221 CGATCCCCGG GAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA

 2281 TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC

 2341 CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT

 2401 TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA

 2461 AAGAAATGCA TAAGCTTTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT

 2521 CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG

 2581 TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT

 2641 CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA

 2701 AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC AGAATTGGTT AATTGGTTGT

 2761 AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGG CTTTGTTGAA TAAATCGAAC

 2821 TTTTGCTGAG TTGAAGGATC AGATCACGCA TCTTCCCGAC AACGCAGACC GTTCCGTGGC

 2881 AAAGCAAAAG TTCAAAATCA CCAACTGGTC CACCTACAAC AAAGCTCTCA TCAACCGTGG

 2941 CTCCCTCACT TTCTGGCTGG ATGATGGGGC GATTCAGGCC TGGTATGAGT CAGCAACACC

 3001 TTCTTCACGA GGCAGACCTC AGCGCTCAAA GATGCAGGGG TAAAAGCTAA CCGCATCTTT

 3061 ACCGACAAGG CATCCGGCAG TTCAACAGAT CGGGAAGGGC TGGATTTGCT GAGGATGAAG

 3121 GTGGAGGAAG GTGATGTCAT TCTGGTGAAG AAGCTCGACC GTCTTGGCCG CGACACCGCC

 3181 GACATGATCC AACTGATAAA AGAGTTTGAT GCTCAGGGTG TAGCGGTTCG GTTTATTGAC

 3241 GACGGGATCA GTACCGACGG TGATATGGGG CAAATGGTGG TCACCATCCT GTCGGCTGTG

 3301 GCACAGGCTG AACGCCGGAG GATCCTAGAG CGCACGAATG AGGGCCGACA GGAAGCAAAG

 3361 CTGAAAGGAA TCAAATTTGG CCGCAGGCGT ACCGTGGACA GGAACGTCGT GCTGACGCTT

 3421 CATCAGAAGG GCACTGGTGC AACGGAAATT GCTCATCAGC TCAGTATTGC CCGCTCCACG

 3481 GTTTATAAAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG

 3541 TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA

 3601 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC

 3661 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG

 3721 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC

 3781 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG

 3841 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA

 3901 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG T

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