pET-24b(+)
pET-24b(+)
编号 | 载体名称 |
北京华越洋生物VECT5050 | pET-24b(+) |
pET24b载体基本信息
别名: | pET24b, pet 24b |
质粒类型: | 大肠杆菌蛋白表达 |
表达水平: | 高 |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 5309 bp |
5' 测序引物: | T7 |
5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
载体标签: | N-T7, C-His |
载体抗性: | Kanamycin |
备注: | Same as pET21a,b,c,d(+) but kanamycin resistance; a,b,c,d vary by MCS; The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. |
稳定性: | 瞬时表达 Transient |
组成型: | 组成型 Constitutive |
病毒/非病毒: | 非病毒 |
pET24b载体质粒图谱和多克隆位点信息
The maps for pET-24b(+), pET-24c(+) and pET-24d(+) are the same as pET-24a(+) (shown) with the following exceptions: pET-24b(+) is a 5309bp plasmid; subtract 1bp from each site beyond BamH I at 198. pET-24c(+) is a 5308bp plasmid; subtract 2bp from each site beyond BamH I at 198. pET-24d(+) is a 5307bp plasmid; the BamH I site is in the same reading frame as in pET- 24c(+). An Nco I site is substituted for the Nde I site with a net 1bp deletion at position 238 of pET-24c(+). As a result, Nco I cuts pET24d(+) at 234, and Nhe I cuts at 229. For the rest of the sites, subtract 3bp from each site beyond position 239 in pET-24a(+). Nde I does not cut pET-24d(+). Note also that Sty I is not unique in pET-24d(+).
Feature Name | Start | End | |
T7_terminator | 129 | 1 | |
T7_Terminal_primer | 69 | 87 | |
T7_leader | 238 | 206 | |
Xpress_fwd_primer | 239 | 221 | |
T7_transl_en_RBS | 263 | 247 | |
lacO | 308 | 281 | |
T7_promoter | 326 | 308 | |
tet (300 - 563) | 358 | 621 | |
pBRrevBam_primer | 429 | 410 | |
lacI | 704 | 1795 | |
tet (576 - 851) | 1854 | 2129 | |
ROP | 2604 | 2795 | |
pGEX_3_primer | 2811 | 2789 | |
pBR322_origin | 3829 | 3210 | |
KanR2 | 3935 | 4750 |
ORF | Start | End | |
ORF frame 1 | 463 | 963 | |
ORF frame 2 | 836 | 1795 | |
ORF frame 2 | 3935 | 4750 |
Enzyme Name | Cut |
XhoI | 158 |
NotI | 166 |
EagI | 166 |
HindIII | 173 |
SalI | 179 |
SacI | 190 |
EcoRI | 192 |
BamHI | 198 |
NheI | 230 |
NdeI | 237 |
XbaI | 275 |
BglII | 341 |
BclI | 1077 |
ApaI | 1274 |
EcoRV | 1513 |
HpaI | 1569 |
FspI | 2145 |
NruI | 4023 |
ClaI | 4057 |
XmaI | 4238 |
SmaI | 4240 |
pET24b载体简介
The pET-24a-d(+) vectors carry an N-terminal T7•Tag® sequence plus an optional C-terminal His•Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs. ampicillin resistance). Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, singlestranded sequencing should be performed using the T7 terminator primer.
pET24b载体序列
ORIGIN
1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT
181 CGACGGAGCT CGAATTCGGA TCCCGACCCA TTTGCTGTCC ACCAGTCATG CTAGCCATAT
241 GTATATCTCC TTCTTAAAGT TAAACAAAAT TATTTCTAGA GGGGAATTGT TATCCGCTCA
301 CAATTCCCCT ATAGTGAGTC GTATTAATTT CGCGGGATCG AGATCTCGAT CCTCTACGCC
361 GGACGCATCG TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC
421 GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC TTGTTTCGGC
481 GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG GCGCCATCTC CTTGCATGCA
541 CCATTCCTTG CGGCGGCGGT GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG
601 CAGGAGTCGC ATAAGGGAGA GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT
661 TCGCGGTATG GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC
721 AGTAACGTTA TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT
781 GGTGAACCAG GCCAGCCACG TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC
841 GGAGCTGAAT TACATTCCCA ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT
901 GATTGGCGTT GCCACCTCCA GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT
961 TAAATCTCGC GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG
1021 CGTCGAAGCC TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT
1081 CATTAACTAT CCGCTGGATG ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT
1141 TCCGGCGTTA TTTCTTGATG TCTCTGACCA GACACCCATC AACAGTATTA TTTTCTCCCA
1201 TGAAGACGGT ACGCGACTGG GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC
1261 GCTGTTAGCG GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA
1321 ATATCTCACT CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT GGAGTGCCAT
1381 GTCCGGTTTT CAACAAACCA TGCAAATGCT GAATGAGGGC ATCGTTCCCA CTGCGATGCT
1441 GGTTGCCAAC GATCAGATGG CGCTGGGCGC AATGCGCGCC ATTACCGAGT CCGGGCTGCG
1501 CGTTGGTGCG GATATCTCGG TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT
1561 CCCGCCGTTA ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG
1621 CTTGCTGCAA CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC CCGTCTCACT
1681 GGTGAAAAGA AAAACCACCC TGGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC
1741 CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA
1801 ACGCAATTAA TGTAAGTTAG CTCACTCATT AGGCACCGGG ATCTCGACCG ATGCCCTTGA
1861 GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC
1921 TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG CTCTGGGTCA
1981 TTTTCGGCGA GGACCGCTTT CGCTGGAGCG CGACGATGAT CGGCCTGTCG CTTGCGGTAT
2041 TCGGAATCTT GCACGCCCTC GCTCAAGCCT TCGTCACTGG TCCCGCCACC AAACGTTTCG
2101 GCGAGAAGCA GGCCATTATC GCCGGCATGG CGGCCCCACG GGTGCGCATG ATCGTGCTCC
2161 TGTCGTTGAG GACCCGGCTA GGCTGGCGGG GTTGCCTTAC TGGTTAGCAG AATGAATCAC
2221 CGATACGCGA GCGAACGTGA AGCGACTGCT GCTGCAAAAC GTCTGCGACC TGAGCAACAA
2281 CATGAATGGT CTTCGGTTTC CGTGTTTCGT AAAGTCTGGA AACGCGGAAG TCAGCGCCCT
2341 GCACCATTAT GTTCCGGATC TGCATCGCAG GATGCTGCTG GCTACCCTGT GGAACACCTA
2401 CATCTGTATT AACGAAGCGC TGGCATTGAC CCTGAGTGAT TTTTCTCTGG TCCCGCCGCA
2461 TCCATACCGC CAGTTGTTTA CCCTCACAAC GTTCCAGTAA CCGGGCATGT TCATCATCAG
2521 TAACCCGTAT CGTGAGCATC CTCTCTCGTT TCATCGGTAT CATTACCCCC ATGAACAGAA
2581 ATCCCCCTTA CACGGAGGCA TCAGTGACCA AACAGGAAAA AACCGCCCTT AACATGGCCC
2641 GCTTTATCAG AAGCCAGACA TTAACGCTTC TGGAGAAACT CAACGAGCTG GACGCGGATG
2701 AACAGGCAGA CATCTGTGAA TCGCTTCACG ACCACGCTGA TGAGCTTTAC CGCAGCTGCC
2761 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
2821 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
2881 TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG CGATAGCGGA GTGTATACTG
2941 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATAT GCGGTGTGAA
3001 ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC
3061 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG
3121 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC
3181 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC
3241 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA
3301 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC
3361 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT
3421 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG
3481 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC
3541 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA
3601 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT
3661 AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
3721 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG
3781 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG
3841 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAA CAATAAAACT
3901 GTCTGCTTAC ATAAACAGTA ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC
3961 TTGCTCTAGG CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC
4021 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGA TTGTATGGGA AGCCCGATGC
4081 GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT
4141 GGTCAGACTA AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG
4201 TACTCCTGAT GATGCATGGT TACTCACCAC TGCGATCCCC GGGAAAACAG CATTCCAGGT
4261 ATTAGAAGAA TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG
4321 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT
4381 CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA
4441 GCGTAATGGC TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAACTTT TGCCATTCTC
4501 ACCGGATTCA GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG
4561 GAAATTAATA GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT
4621 TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA
4681 AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA
4741 GTTTTTCTAA GAATTAATTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC
4801 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGAAATTGTA AACGTTAATA
4861 TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG
4921 AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC
4981 CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA
5041 CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT
5101 CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC
5161 GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA
5221 GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG
5281 CGCCGCTACA GGGCGCGTCC CATTCGCCA
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其他大肠杆菌表达载体：
