pACYCDuet-1

pACYCDuet1载体基本信息

pACYCDuet1载体质粒图谱和多克隆位点信息

pACYCDuet1载体简介

pACYCDue-1 is designed for the coexpression of two target genes. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7 promoter/lac operator and ribosome binding site (rbs). The vector also carries the P15A replicon, lacI gene and chloramphenicol resistance gene. This vector can be used in combination with pACYCDuet™-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of up to 4 target genes. Genes inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer (Cat. No. 71178-3) and DuetDOWN1 Primer (Cat. No. 71179- 3). Genes inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3).

pACYCDuet1载体序列

ORIGIN

    1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag

   61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag

  121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag

  181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt

  241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata

  301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt

  361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg

  421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg

  481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg

  541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt

  601 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat

  661 ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct

  721 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt

  781 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc

  841 accttgtcgc cttgcgtata atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc

  901 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa

  961 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca

 1021 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat

 1081 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc

 1141 accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca

 1201 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag

 1261 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc

 1321 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt

 1381 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt

 1441 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat

 1501 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat

 1561 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct

 1621 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc

 1681 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc

 1741 cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc

 1801 agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt

 1861 gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc

 1921 ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac

 1981 ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga

 2041 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag

 2101 ataccaggcg tttcccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt

 2161 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc

 2221 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg

 2281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc

 2341 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt

 2401 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt

 2461 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt

 2521 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc

 2581 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca

 2641 gccccatacg atataagttg taattctcat gttagtcatg ccccgcgccc accggaagga

 2701 gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag

 2761 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg

 2821 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca

 2881 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc

 2941 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt

 3001 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta

 3061 ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg

 3121 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca

 3181 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa

 3241 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac

 3301 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc

 3361 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga

 3421 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt

 3481 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg

 3541 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca

 3601 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac

 3661 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt

 3721 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa

 3781 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg

 3841 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc

 3901 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc

 3961 tctcccttat gcgactcctg cattaggaaa ttaatacgac tcactata

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