pET-32 Xa/LIC
pET-32 Xa/LIC
编号 | 载体名称 |
北京华越洋生物VECT4510 | pET-32 Xa/LIC |
pET32 Xa/LIC载体基本信息
别名: | pET32 Xa/LIC, pet 32 Xa/LIC |
质粒类型: | 大肠杆菌蛋白表达 |
表达水平: | 高 |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 5926 bp |
5' 测序引物: | T7或者Trx-F |
5' 测序引物序列: | T7: 5'-TAATACGACTCACTATAGGG-3'; Trx-F: 5' TTCCTCGACGCTAACCTG 3' |
载体标签: | thioredoxin (N端); His (中间和C端), N- Factor Xa |
载体抗性: | Ampicillin |
备注: | For directional cloning of PCR-amplified DNA; ligation independent cloning; Factor Xa cleavage site |
稳定性: | 瞬时表达 Transient |
组成型: | 组成型 Constitutive |
病毒/非病毒: | 非病毒 |
pET32 Xa/LIC载体质粒图谱和多克隆位点信息
pET32 Xa/LIC载体简介
The pET-32 Xa/LIC vector is designed for rapid, directional cloning of PCR-amplified DNA for high-level expression of polypeptides fused with the 109aa Trx•Tag™ thioredoxin protein (1), His•Tag® and S•Tag™ sequences. Using specifically designed primers for amplification and the pET-32 Xa/LIC Vector Kit (Cat. No. 69072-3), inserts can be efficiently cloned without the need for restriction digestion or ligation, and the resulting fusion proteins can be cleaved with Factor Xa to precisely remove all vector-encoded amino acids. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).
pET32 Xa/LIC载体序列
ORIGIN
1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT
181 CGACGGAGCT CGAATTCGGA TCCGATATCG CCATGGCCAG AGGAGAGTTA GAGCGACCCT
241 CAATACCGGA GCCACCACCG GTACCCAGAT CTGGGCTGTC CATGTGCTGG CGTTCGAATT
301 TAGCAGCAGC GGTTTCTTTC ATACCAGAAC CGCGTGGCAC CAGACCAGAA GAATGATGAT
361 GATGATGGTG CATATGGCCA GAACCAGAAC CGGCCAGGTT AGCGTCGAGG AACTCTTTCA
421 ACTGACCTTT AGACAGTGCA CCCACTTTGG TTGCCGCCAC TTCACCGTTT TTGAACAGCA
481 GCAGAGTCGG GATACCACGG ATGCCATATT TCGGCGCAGT GCCAGGGTTT TGATCGATGT
541 TCAGTTTTGC AACGGTCAGT TTGCCCTGAT ATTCGTCAGC GATTTCATCC AGAATCGGGG
601 CGATCATTTT GCACGGACCG CACCACTCTG CCCAGAAATC GACGAGGATC GCCCCGTCCG
661 CTTTGAGTAC ATCCGTGTCA AAACTGTCGT CAGTCAGGTG AATAATTTTA TCGCTCATAT
721 GTATATCTCC TTCTTAAAGT TAAACAAAAT TATTTCTAGA GGGGAATTGT TATCCGCTCA
781 CAATTCCCCT ATAGTGAGTC GTATTAATTT CGCGGGATCG AGATCGATCT CGATCCTCTA
841 CGCCGGACGC ATCGTGGCCG GCATCACCGG CGCCACAGGT GCGGTTGCTG GCGCCTATAT
901 CGCCGACATC ACCGATGGGG AAGATCGGGC TCGCCACTTC GGGCTCATGA GCGCTTGTTT
961 CGGCGTGGGT ATGGTGGCAG GCCCCGTGGC CGGGGGACTG TTGGGCGCCA TCTCCTTGCA
1021 TGCACCATTC CTTGCGGCGG CGGTGCTCAA CGGCCTCAAC CTACTACTGG GCTGCTTCCT
1081 AATGCAGGAG TCGCATAAGG GAGAGCGTCG AGATCCCGGA CACCATCGAA TGGCGCAAAA
1141 CCTTTCGCGG TATGGCATGA TAGCGCCCGG AAGAGAGTCA ATTCAGGGTG GTGAATGTGA
1201 AACCAGTAAC GTTATACGAT GTCGCAGAGT ATGCCGGTGT CTCTTATCAG ACCGTTTCCC
1261 GCGTGGTGAA CCAGGCCAGC CACGTTTCTG CGAAAACGCG GGAAAAAGTG GAAGCGGCGA
1321 TGGCGGAGCT GAATTACATT CCCAACCGCG TGGCACAACA ACTGGCGGGC AAACAGTCGT
1381 TGCTGATTGG CGTTGCCACC TCCAGTCTGG CCCTGCACGC GCCGTCGCAA ATTGTCGCGG
1441 CGATTAAATC TCGCGCCGAT CAACTGGGTG CCAGCGTGGT GGTGTCGATG GTAGAACGAA
1501 GCGGCGTCGA AGCCTGTAAA GCGGCGGTGC ACAATCTTCT CGCGCAACGC GTCAGTGGGC
1561 TGATCATTAA CTATCCGCTG GATGACCAGG ATGCCATTGC TGTGGAAGCT GCCTGCACTA
1621 ATGTTCCGGC GTTATTTCTT GATGTCTCTG ACCAGACACC CATCAACAGT ATTATTTTCT
1681 CCCATGAAGA CGGTACGCGA CTGGGCGTGG AGCATCTGGT CGCATTGGGT CACCAGCAAA
1741 TCGCGCTGTT AGCGGGCCCA TTAAGTTCTG TCTCGGCGCG TCTGCGTCTG GCTGGCTGGC
1801 ATAAATATCT CACTCGCAAT CAAATTCAGC CGATAGCGGA ACGGGAAGGC GACTGGAGTG
1861 CCATGTCCGG TTTTCAACAA ACCATGCAAA TGCTGAATGA GGGCATCGTT CCCACTGCGA
1921 TGCTGGTTGC CAACGATCAG ATGGCGCTGG GCGCAATGCG CGCCATTACC GAGTCCGGGC
1981 TGCGCGTTGG TGCGGACATC TCGGTAGTGG GATACGACGA TACCGAAGAC AGCTCATGTT
2041 ATATCCCGCC GTTAACCACC ATCAAACAGG ATTTTCGCCT GCTGGGGCAA ACCAGCGTGG
2101 ACCGCTTGCT GCAACTCTCT CAGGGCCAGG CGGTGAAGGG CAATCAGCTG TTGCCCGTCT
2161 CACTGGTGAA AAGAAAAACC ACCCTGGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT
2221 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG
2281 CGCAACGCAA TTAATGTAAG TTAGCTCACT CATTAGGCAC CGGGATCTCG ACCGATGCCC
2341 TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC GGGGCATGAC TATCGTCGCC
2401 GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC AGGTGCCGGC AGCGCTCTGG
2461 GTCATTTTCG GCGAGGACCG CTTTCGCTGG AGCGCGACGA TGATCGGCCT GTCGCTTGCG
2521 GTATTCGGAA TCTTGCACGC CCTCGCTCAA GCCTTCGTCA CTGGTCCCGC CACCAAACGT
2581 TTCGGCGAGA AGCAGGCCAT TATCGCCGGC ATGGCGGCCC CACGGGTGCG CATGATCGTG
2641 CTCCTGTCGT TGAGGACCCG GCTAGGCTGG CGGGGTTGCC TTACTGGTTA GCAGAATGAA
2701 TCACCGATAC GCGAGCGAAC GTGAAGCGAC TGCTGCTGCA AAACGTCTGC GACCTGAGCA
2761 ACAACATGAA TGGTCTTCGG TTTCCGTGTT TCGTAAAGTC TGGAAACGCG GAAGTCAGCG
2821 CCCTGCACCA TTATGTTCCG GATCTGCATC GCAGGATGCT GCTGGCTACC CTGTGGAACA
2881 CCTACATCTG TATTAACGAA GCGCTGGCAT TGACCCTGAG TGATTTTTCT CTGGTCCCGC
2941 CGCATCCATA CCGCCAGTTG TTTACCCTCA CAACGTTCCA GTAACCGGGC ATGTTCATCA
3001 TCAGTAACCC GTATCGTGAG CATCCTCTCT CGTTTCATCG GTATCATTAC CCCCATGAAC
3061 AGAAATCCCC CTTACACGGA GGCATCAGTG ACCAAACAGG AAAAAACCGC CCTTAACATG
3121 GCCCGCTTTA TCAGAAGCCA GACATTAACG CTTCTGGAGA AACTCAACGA GCTGGACGCG
3181 GATGAACAGG CAGACATCTG TGAATCGCTT CACGACCACG CTGATGAGCT TTACCGCAGC
3241 TGCCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG
3301 GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG
3361 GGTGTTGGCG GGTGTCGGGG CGCAGCCATG ACCCAGTCAC GTAGCGATAG CGGAGTGTAT
3421 ACTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATATGCGGTG
3481 TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCTCTT CCGCTTCCTC
3541 GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA
3601 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA
3661 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT
3721 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
3781 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
3841 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
3901 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
3961 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
4021 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
4081 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
4141 CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
4201 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
4261 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC
4321 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC
4381 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG
4441 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC
4501 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC
4561 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC
4621 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG
4681 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG
4741 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTGCAGGCA TCGTGGTGTC
4801 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC
4861 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG
4921 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC
4981 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG
5041 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC
5101 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT
5161 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG
5221 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA
5281 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT
5341 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG
5401 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA
5461 AATTGTAAAC GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT
5521 TTTTAACCAA TAGGCCGAAA TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT
5581 AGGGTTGAGT GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA
5641 CGTCAAAGGG CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA
5701 ATCAAGTTTT TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC
5761 CCGATTTAGA GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC
5821 GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC
5881 ACCCGCCGCG CTTAATGCGC CGCTACAGGG CGCGTCCCAT TCGCCA
//
其他大肠杆菌表达载体：
pGEX-2T
编号 | 载体名称 |
北京华越洋生物VECT4440 | pGEX-2T |
pGEX-2T载体基本信息
别名: | pGEX-2T, pGEX2T, pGEX 2T |
质粒类型: | 大肠杆菌蛋白表达载体 |
表达水平: | 高拷贝 |
启动子: | Tac |
克隆方法: | 多克隆位点，限制性内切酶, BamHI，SmaI, EcoRI, XbaI, NcoI, SalI, XhoI, SacI, HindIII |
载体大小: | 4898 bp |
5' 测序引物及序列: | pGEX5': GGGCTGGCAAGCCACGTTTGGTG |
3' 测序引物及序列: | pGEX3': CCGGGAGCTGCATGTGTCAGAGG |
载体标签: | N-GST |
载体抗性: | Ampicillin 氨苄 |
备注: | 复制子是pMB1 |
稳定性: | 瞬时表达 Transient |
组成型: | 诱导表达 |
病毒/非病毒: | 非病毒 |
pGEX-2T载体质粒图谱和多克隆位点信息
pGEX系列载体：
pGEX-1λT EcoRI/BAP
pGEX-2T
pGEX-2TK
pGEX-20T
pGEX-3X
pGEX-4T-1
pGEX-4T-2
pGEX-4T-3
pGEX-5X-1
pGEX-5X-2
pGEX-5X-3
pGEX-6P-1
pGEX-6P-2
pGEX-6P-3
pGEX-KG
pGEX-2T载体序列
ORIGIN
1 ACGTTATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC GGAAGCTGTG
61 GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC GCACTCCCGT
121 TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC TGAAATGAGC
181 TGTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATT GTGAGCGGAT AACAATTTCA
241 CACAGGAAAC AGTATTCATG TCCCCTATAC TAGGTTATTG GAAAATTAAG GGCCTTGTGC
301 AACCCACTCG ACTTCTTTTG GAATATCTTG AAGAAAAATA TGAAGAGCAT TTGTATGAGC
361 GCGATGAAGG TGATAAATGG CGAAACAAAA AGTTTGAATT GGGTTTGGAG TTTCCCAATC
421 TTCCTTATTA TATTGATGGT GATGTTAAAT TAACACAGTC TATGGCCATC ATACGTTATA
481 TAGCTGACAA GCACAACATG TTGGGTGGTT GTCCAAAAGA GCGTGCAGAG ATTTCAATGC
541 TTGAAGGAGC GGTTTTGGAT ATTAGATACG GTGTTTCGAG AATTGCATAT AGTAAAGACT
601 TTGAAACTCT CAAAGTTGAT TTTCTTAGCA AGCTACCTGA AATGCTGAAA ATGTTCGAAG
661 ATCGTTTATG TCATAAAACA TATTTAAATG GTGATCATGT AACCCATCCT GACTTCATGT
721 TGTATGACGC TCTTGATGTT GTTTTATACA TGGACCCAAT GTGCCTGGAT GCGTTCCCAA
781 AATTAGTTTG TTTTAAAAAA CGTATTGAAG CTATCCCACA AATTGATAAG TACTTGAAAT
841 CCAGCAAGTA TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC
901 ATCCTCCAAA ATCGGATCTG GTTCCGCGTG GATCCCCGGG AATTCATCGT GACTGACTGA
961 CGATCTGCCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG
1021 AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT
1081 CAGCGGGTGT TGGCGGGTGT CGGGGCGCAG CCATGACCCA GTCACGTAGC GATAGCGGAG
1141 TGTATAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA
1201 TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC
1261 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT
1321 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG
1381 CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG
1441 TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC
1501 TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA
1561 CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC
1621 TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA
1681 AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG
1741 ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT
1801 TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG
1861 AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC
1921 GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA
1981 TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA
2041 TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC
2101 CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG
2161 ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT
2221 CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA
2281 GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT
2341 CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT
2401 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT
2461 TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA
2521 TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG
2581 CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA
2641 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG
2701 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA
2761 GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA
2821 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA
2881 ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT
2941 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC
3001 GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT
3061 CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA
3121 CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC
3181 TTACGCATCT GTGCGGTATT TCACACCGCA TAAATTCCGA CACCATCGAA TGGTGCAAAA
3241 CCTTTCGCGG TATGGCATGA TAGCGCCCGG AAGAGAGTCA ATTCAGGGTG GTGAATGTGA
3301 AACCAGTAAC GTTATACGAT GTCGCAGAGT ATGCCGGTGT CTCTTATCAG ACCGTTTCCC
3361 GCGTGGTGAA CCAGGCCAGC CACGTTTCTG CGAAAACGCG GGAAAAAGTG GAAGCGGCGA
3421 TGGCGGAGCT GAATTACATT CCCAACCGCG TGGCACAACA ACTGGCGGGC AAACAGTCGT
3481 TGCTGATTGG CGTTGCCACC TCCAGTCTGG CCCTGCACGC GCCGTCGCAA ATTGTCGCGG
3541 CGATTAAATC TCGCGCCGAT CAACTGGGTG CCAGCGTGGT GGTGTCGATG GTAGAACGAA
3601 GCGGCGTCGA AGCCTGTAAA GCGGCGGTGC ACAATCTTCT CGCGCAACGC GTCAGTGGGC
3661 TGATCATTAA CTATCCGCTG GATGACCAGG ATGCCATTGC TGTGGAAGCT GCCTGCACTA
3721 ATGTTCCGGC GTTATTTCTT GATGTCTCTG ACCAGACACC CATCAACAGT ATTATTTTCT
3781 CCCATGAAGA CGGTACGCGA CTGGGCGTGG AGCATCTGGT CGCATTGGGT CACCAGCAAA
3841 TCGCGCTGTT AGCGGGCCCA TTAAGTTCTG TCTCGGCGCG TCTGCGTCTG GCTGGCTGGC
3901 ATAAATATCT CACTCGCAAT CAAATTCAGC CGATAGCGGA ACGGGAAGGC GACTGGAGTG
3961 CCATGTCCGG TTTTCAACAA ACCATGCAAA TGCTGAATGA GGGCATCGTT CCCACTGCGA
4021 TGCTGGTTGC CAACGATCAG ATGGCGCTGG GCGCAATGCG CGCCATTACC GAGTCCGGGC
4081 TGCGCGTTGG TGCGGATATC TCGGTAGTGG GATACGACGA TACCGAAGAC AGCTCATGTT
4141 ATATCCCGCC GTTAACCACC ATCAAACAGG ATTTTCGCCT GCTGGGGCAA ACCAGCGTGG
4201 ACCGCTTGCT GCAACTCTCT CAGGGCCAGG CGGTGAAGGG CAATCAGCTG TTGCCCGTCT
4261 CACTGGTGAA AAGAAAAACC ACCCTGGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT
4321 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG
4381 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG
4441 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC
4501 TATGACCATG ATTACGGATT CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC
4561 TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG
4621 CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGCG
4681 CTTTGCCTGG TTTCCGGCAC CAGAAGCGGT GCCGGAAAGC TGGCTGGAGT GCGATCTTCC
4741 TGAGGCCGAT ACTGTCGTCG TCCCCTCAAA CTGGCAGATG CACGGTTACG ATGCGCCCAT
4801 CTACACCAAC GTAACCTATC CCATTACGGT CAATCCGCCG TTTGTTCCCA CGGAGAATCC
4861 GACGGGTTGT TACTCGCTCA CATTTAATGT TGATGAAAGC TGGCTACAGG AAGGCCAGAC
4921 GCGAATTATT TTTGATGGCG TTGGAATT
//
pGEX-2T其他大肠杆菌表达载体：
