pLKO.1 GFP shRNA
pLKO.1 GFP shRNA
编号 | 载体名称 |
北京华越洋VECT75616 | pLKO.1 GFP shRNA |
pLKO.1 GFP shRNA载体基本信息：
启动子: | U6，hPGK,T7 |
复制子: | pUC ori,f1 ori,SV40 ori |
终止子: | cPPT/CTS |
质粒分类: | 病毒系列，慢病毒干扰载体 |
质粒大小: | 7091bp |
质粒标签: | GFP shRNA |
原核抗性: | Amp |
筛选标记: | Puro |
克隆菌株: | Stbl3 |
培养条件: | 37℃，有氧 LB |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | U6:ATGGACTATCATATGCTTACCGTA |
3'测序引物: | 根据序列设计引物 |
pLKO.1 GFP shRNA载体图谱：
pLKO.1 GFP shRNA载体简介：
PLKO.1 GFP shRNA pLKO.1 Puro Mammalian plasmid background, which belongs to Expression, Lentiviral, RNAi, we are in the transformation, the clone strain Stbl3, and to use a medium containing ampicillin were cultured under 37 degrees.
pLKO.1 GFP shRNA载体序列：
ORIGIN
1 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
61 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
301 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
361 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
421 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
481 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
541 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
601 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
661 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
721 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
781 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
841 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
901 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
961 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
1021 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
1081 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
1141 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
1201 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
1261 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
1321 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
1381 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
1441 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
1501 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1561 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1621 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1681 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1741 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1801 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1861 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1921 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1981 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
2041 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
2101 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
2161 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
2221 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
2281 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
2341 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac gagactagcc
2401 tcgagcggcc gcccccttca ccgagggcct atttcccatg attccttcat atttgcatat
2461 acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt
2521 agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt
2581 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
2641 tttatatatc ttgtggaaag gacgaaacac cgggcccgca agctgaccct gaagttcatt
2701 caagagatga acttcagggt cagcttgctt tttgaattct cgacctcgag acaaatggca
2761 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
2821 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
2881 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag
2941 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
3001 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
3061 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
3121 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
3181 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
3241 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
3301 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
3361 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
3421 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggagctta ccatgaccga
3481 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
3541 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
3601 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
3661 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
3721 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
3781 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
3841 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
3901 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
3961 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
4021 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
4081 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcgg tacctttaag
4141 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
4201 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
4261 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
4321 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
4381 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
4441 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
4501 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
4561 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
4621 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
4681 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
4741 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
4801 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
4861 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
4921 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
4981 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
5041 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
5101 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
5161 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
5221 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
5281 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
5341 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
5401 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
5461 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
5521 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
5581 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
5641 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
5701 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
5761 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
5821 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
5881 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
5941 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
6001 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
6061 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
6121 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
6181 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
6241 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
6301 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
6361 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
6421 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
6481 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
6541 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
6601 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
6661 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
6721 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
6781 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
6841 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
6901 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
6961 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
7021 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
7081 cggaacagga g
//
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