pLJM1
pLJM1
编号 | 载体名称 |
北京华越洋VECT75623 | pLJM1 |
pLJM1载体基本信息：
启动子: | hPGK ,CMV,T3 |
复制子: | SV40 Ori，F1 Ori |
终止子: | SV40 poly(A) signal |
质粒分类: | 病毒系列，慢病毒克隆载体 |
质粒大小: | 7400bp |
原核抗性: | Amp |
筛选标记: | Puro |
克隆菌株: | Stbl3 |
培养条件: | 37℃，有氧 LB |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'测序引物: | 根据序列设计引物 |
pLJM1载体图谱：
pLJM1载体序列：
ORIGIN
1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
361 gcgctaccgg tatggactac aaggatgacg atgacaaggg tggtggtggt ggtgcgtcga
421 cnnnnnnnnn nngatatctt cgaattctcg acctcgagac aaatggcagt attcatccac
481 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
541 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
601 cgggtttatt acagggacag cagagatcca ctttggccgc ggctcgaggg ggttggggtt
661 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
721 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
781 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
841 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
901 tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
961 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
1021 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
1081 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
1141 caccgacctc tctccccagg gggatccacc ggagcttacc atgaccgagt acaagcccac
1201 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
1261 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
1321 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
1381 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
1441 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
1501 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
1561 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
1621 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
1681 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
1741 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
1801 gcgcccgacc gaaaggagcg cacgacccca tgcatcggta cctttaagac caatgactta
1861 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
1921 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
1981 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
2041 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
2101 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
2161 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
2221 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
2281 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
2341 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
2401 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
2461 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
2521 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
2581 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
2641 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
2701 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
2761 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
2821 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
2881 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
2941 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
3001 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
3061 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
3121 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
3181 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
3241 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
3301 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
3361 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
3421 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
3481 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
3541 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
3601 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
3661 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
3721 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
3781 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
3841 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
3901 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
3961 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
4021 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
4081 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
4141 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
4201 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
4261 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
4321 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
4381 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
4441 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
4501 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
4561 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
4621 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
4681 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
4741 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
4801 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
4861 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
4921 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
4981 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
5041 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
5101 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
5161 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
5221 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
5281 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
5341 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
5401 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
5461 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
5521 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
5581 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
5641 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
5701 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
5761 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
5821 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
5881 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
5941 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
6001 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
6061 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
6121 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
6181 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
6241 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
6301 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
6361 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
6421 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
6481 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
6541 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
6601 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
6661 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
6721 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
6781 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
6841 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
6901 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
6961 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
7021 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
7081 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
7141 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgatcacg agactagcct
7201 cgagcggccg cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata
7261 cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta
7321 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
7381 tgttttaaaa tggactatca
//
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