YEP352
YEP352
编号 | 载体名称 |
北京华越洋VECT75548 | YEP352 |
YEP352载体基本信息：
启动子: | URA3 |
复制子: | 2μ ori,ori |
质粒分类: | 酵母系列，酿酒酵母表达载体 |
质粒大小: | 5881bp |
原核抗性: | Amp |
筛选标记: | URA3 |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧 LB |
表达宿主: | 酵母细胞 |
5'测序引物: | M13F:TGTAAAACGACGGCCAGT |
3'测序引物: | 根据序列设计引物 |
YEP352载体图谱：
YEP352载体简介：
Restriction digests of the clone give the following sizes (kb): BamHI--5.3; XbaI--5.3; PstI--5.3; HindIII--5.3.This is a Escherichia coli/yeast episomal shuttle vector with multiple restriction sites for cloning.Constructed by filling in and blunt-end ligating pUC18 linearized at NdeI, a 1.4 kb RsaI fragment of the 2 micron circle, and a 1.1 kb HindIII/SmaI fragment containing URA3. A XbaI site in 2u and a PstI site in URA3 were removed.The yeast genomicsequence is in nt 286-1383 (where the A of the ATG of lacZ' is number 1), the 2 micron sequence goes from nt 1384-2768, and pUC18 includes nt 2769-285.
YEP352载体序列：
ORIGIN
1 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
61 gcataaagtg taaagcctgg ggtgcctaat gagtgaggta actcacatta attgcgttgc
121 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctggattaa tgaatcggcc
181 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
241 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
301 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
361 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
421 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
481 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
541 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
601 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
661 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
721 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
781 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
841 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
901 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
961 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
1021 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
1081 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
1141 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
1201 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
1261 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
1321 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
1381 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
1441 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
1501 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
1561 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
1621 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
1681 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
1741 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
1801 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
1861 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
1921 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
1981 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
2041 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
2101 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
2161 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
2221 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
2281 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
2341 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
2401 ataacgcatt taagcataaa cacgcactat gccgttcttc tcatgtatat atatatacag
2461 gcaacacgca gatataggtg cgacgtgaac agtgagctgt atgtgcgcag ctcgcgttgc
2521 attttcggaa gcgctcgttt tcggaaacgc tttgaagttc ctattccgaa gttcctattc
2581 tctagctaga aagtatagga acttcagagc gcttttgaaa accaaaagcg ctctgaagac
2641 gcactttcaa aaaaccaaaa acgcaccgga ctgtaacgag ctactaaaat attgcgaata
2701 ccgcttccac aaacattgct caaaagtatc tctttgctat atatctctgt gctatatccc
2761 tatataacct acccatccac ctttcgctcc ttgaacttgc atctaaactc gacctctaca
2821 ttttttatgt ttatctctag tattactctt tagacaaaaa aattgtagta agaactattc
2881 atagagtgaa tcgaaaacaa tacgaaaatg taaacatttc ctatacgtag tatatagaga
2941 caaaatagaa gaaaccgttc ataattttct gaccaatgaa gaatcatcaa cgctatcact
3001 ttctgttcac aaagtatgcg caatccacat cggtatagaa tataatcggg gatgccttta
3061 tcttgaaaaa atgcacccgc agcttcgcta gtaatcagta aacgcgggaa gtggagtcag
3121 gcttttttta tggaagagaa aatagacacc aaagtagcct tcttctaacc ttaacggacc
3181 tacagtgcaa aaagttatca agagactgca ttatagagcg cacaaaggag aaaaaaagta
3241 atctaagatg ctttgttaga aaaatagcgc tctcgggatg catttttgta gaacaaaaaa
3301 gaagtataga ttctttgttg gtaaaatagc gctctcgcgt tgcatttctg ttctgtaaaa
3361 atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt ttgttttaca
3421 aaaatgaagc acagattctt cgttggtaaa atagcgcttt cgcgttgcat ttctgttctg
3481 taaaaatgca gctcagattc tttgtttgaa aaattagcgc tctcgcgttg catttttgtt
3541 ctacaaaatg aagcacagat gcttcgttaa caaagatatg ctattgaagt gcaagatgga
3601 aacgcagaaa atgaaccggg gatgcgacgt gcaagattac ctatgcaata gatgcaatag
3661 tttctccagg aaccgaaata catacattgt cttccgtaaa gcgctagact atatattatt
3721 atacaggttc aaatatacta tctgtttcag ggaaaactcc caggttcgga tgttcaaaat
3781 tcaatgatgg gtaacaagag cttttcaatt catcattttt tttttattct tttttttgat
3841 ttcggtttct ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga
3901 aggagcacag acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt
3961 gcccagtatt cttaacccaa ctgcacagaa caaaaaccga aacgaagata aatcatgtcg
4021 aaagctacat ataaggaacg tgctgctact catcctagtc ctgttgctgc caagctattt
4081 aatatcatgc acgaaaagca aacaaacttg tgtgcttcat tggatgttcg taccaccaag
4141 gaattactgg agttagttga agcattaggt cccaaaattt gtttactaaa aacacatgtg
4201 gatatcttga ctgatttttc catggagggc acagttaagc cgctaaaggc attatccgcc
4261 aagtacaatt ttttactctt cgaagacaga aaatttgctg acattggtaa tacagtcaaa
4321 ttgcagtact ctgcgggtgt atacagaata gcagaatggg cagacattac gaatgcacac
4381 ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg cggcagaaga agtaacaaag
4441 gaacctagag gccttttgat gttagcagaa ttgtcatgca agggctccct atctactgga
4501 gaatatacta agggtactgt tgacattgcg aagagcgaca aagattttgt tatcggcttt
4561 attgctcaaa gagacatggg tggaagagat gaaggttacg attggttgat tatgacaccc
4621 ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac agtatagaac cgtggatgat
4681 gtggtctcta caggatctga cattattatt gttggaagag gactatttgc aaagggaagg
4741 gatgctaagg tagagggtga acgttacaga aaagcaggct gggaagcata tttgagaaga
4801 tgcggccagc aaaactaaaa aactgtatta taagtaaatg catgtatact aaactcacaa
4861 attagagctt caatttaatt atatcagtta ttaccctatg cggtgtgaaa taccgcacag
4921 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
4981 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaagg ggggatgtgc
5041 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
5101 ggccagtgcc aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct
5161 cgaattcgta atcatggtca t
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