pYES2.0
pYES2.0
编号 | 载体名称 |
北京华越洋VECT75549 | pYES2.0 |
pYES2.0载体基本信息：
启动子: | URA3 ，T7，GAL1 |
复制子: | 2μ ori,ori,FI ori |
终止子: | CYC1 |
质粒分类: | 酵母系列，酿酒酵母表达载体 |
质粒大小: | 5856bp |
原核抗性: | Amp |
筛选标记: | URA3 |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧 LB |
表达宿主: | 酵母细胞 |
诱导方式: | 半乳糖诱导 |
5'测序引物: | GAL1-F: ATTTTCGGTTTGTATTACTTC |
3'测序引物: | GALI-R: GTTCTTAATACTAACATAACT |
pYES2.0载体图谱：
pYES2.0载体简介：
The pYES vectors are available with a variety of epitope tags and selection markers to satisfy your expression needs.The parental pYES2 vector is utilized for native expression of your protein of interest. It contains the URA3 selection marker for selection in yeast.
pYES2.0载体序列：
ORIGIN
1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gctgtaatac
481 gactcactat agggaatatt aagcttggta ccgagctcgg atccactagt aacggccgcc
541 agtgtgctgg aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga
601 gggccgcatc atgtaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc
661 tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat
721 agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag
781 acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc
841 gaaggcttta atttgcggcc ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
901 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
961 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
1021 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa gcccaggaac cgtaaaaagg
1081 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
1141 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
1201 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
1261 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
1321 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
1381 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
1441 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
1501 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
1561 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
1621 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
1681 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
1741 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
1801 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
1861 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
1921 cctgactccc cgtcgtgtag ataactacga tacgggagcg cttaccatct ggccccagtg
1981 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
2041 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
2101 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
2161 ttggcattgc tacaggcatc gtggtgtcac tctcgtcgtt tggtatggct tcattcagct
2221 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
2281 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
2341 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
2401 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
2461 gcccggcgtc aatacgggat aatagtgtat cacatagcag aactttaaaa gtgctcatca
2521 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
2581 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
2641 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
2701 aatgttgaat actcatactc ttcctttttc aatgggtaat aactgatata attaaattga
2761 agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt tttagttttg
2821 ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt caccctctac
2881 cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt cagatcctgt
2941 agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct tgtcatctaa
3001 acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac ccatgtctct
3061 ttgagcaata aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa cagtaccctt
3121 agtatattct ccagtagata gggagccctt gcatgacaat tctgctaaca tcaaaaggcc
3181 tctaggttcc tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa tacctgggcc
3241 caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata cacccgcaga
3301 gtactgcaat ttgactgtat taccaatgtc agcaaatttt ctgtcttcga agagtaaaaa
3361 attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg aaaaatcagt
3421 caagatatcc acatgtgttt ttagtaaaca aattttggga cctaatgctt caactaactc
3481 cagtaattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt gcttttcgtg
3541 catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac gttccttata
3601 tgtagctttc gacatgattt atcttcgttt cctgcaggtt tttgttctgt gcagttgggt
3661 taagaatact gggcaatttc atgtttcttc aacactacat atgcgtatat ataccaatct
3721 aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt
3781 tcaaagaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga attgaaaagc
3841 tagcttatcg atgataagct gtcaaagatg agaattaatt ccacggacta tagactatac
3901 tagatactcc gtctactgta cgatacactt ccgctcaggt ccttgtcctt taacgaggcc
3961 ttaccactct tttgttactc tattgatcca gctcagcaaa ggcagtgtga tctaagattc
4021 tatcttcgcg atgtagtaaa actagctaga ccgagaaaga gactagaaat gcaaaaggca
4081 cttctacaat ggctgccatc attattatcc gatgtgacgc tgcagcttct caatgatatt
4141 cgaatacgct ttgaggagat acagcctaat atccgacaaa ctgttttaca gatttacgat
4201 cgtacttgtt acccatcatt gaattttgaa catccgaacc tgggagtttt ccctgaaaca
4261 gatagtatat ttgaacctgt ataataatat atagtctagc gctttacgga agacaatgta
4321 tgtatttcgg ttcctggaga aactattgca tctattgcat aggtaatctt gcacgtcgca
4381 tccccggttc attttctgcg tttccatctt gcacttcaat agcatatctt tgttaacgaa
4441 gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
4501 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa
4561 cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgacgaga gcgctaattt
4621 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat
4681 tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct
4741 atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag
4801 tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt
4861 ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg
4921 aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg
4981 gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa
5041 attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt
5101 tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag
5161 taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc
5221 gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac
5281 ttttgagcaa tgtttgtgga agcggtattc gcaatgggaa gctccacccc ggttgataat
5341 cagaaaagcc ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat
5401 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa cgaatagccc
5461 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
5521 ccagtttcca acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
5581 agggtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
5641 tcgaggtgcc gtaaagcagt aaatcggaag ggtaaacgga tgcccccatt tagagcttga
5701 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggggct
5761 agggcggtgg gaagtgtagg ggtcacgctg ggcgtaacca ccacacccgc cgcgcttaat
5821 ggggcgctac agggcgcgtg gggatgatcc actagt
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