pMKO.1-GFP
pMKO.1-GFP
编号 | 载体名称 |
北京华越洋VECT55122 | pMKO.1-GFP |
pMKO.1-GFP载体基本信息：
载体名称: | pMKO.1-GFP |
质粒类型: | 逆病毒载体；RNAi载体 |
高拷贝/低拷贝: | 低拷贝 |
克隆方法: | 限制性内切酶，多克隆位点 |
启动子: | U6 |
载体大小: | 6767 bp |
5' 测序引物及序列: | pLXSN 5' primer 5' CCCTTGAACCTCCTCGTTCGACC3' |
3' 测序引物及序列: | pBABE 3' primer 5'ACCCTAACTGACACACATTCC3' |
载体标签: | 无 |
载体抗性: | 氨苄青霉素 |
筛选标记: | EGFP |
克隆菌株: | DH5α、HB101等 |
宿主细胞（系）: | 常规哺乳动物细胞系 |
备注: | 推荐使用逆病毒包装载体：pUMVC、pCMV-VSV-G。 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 逆转录病毒 |
pMKO.1-GFP载体质粒图谱和多克隆位点信息：
pMKO.1-GFP载体序列：
ORIGIN
1 CCATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT
61 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG
121 CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA
181 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TAAGCTTTTT
241 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA
301 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC
361 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA
421 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA
481 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG
541 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCCGG ATCTACACTC CGCTATCGCT
601 ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG
661 GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT
721 GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCCAGCTT ACCTCCCGGT
781 GGTGGGTCGG TGGTCCCTGG GCAGGGGTCT CCCGATCCCG GACGAGCCCC CAAATGAAAG
841 ACCCCCGCTG ACGGGTAGTC AATCACTCAG AGGAGACCCT CCCAAGGAAC AGCGAGACCA
901 CAAGTCGGAT GCAACTGCAA GAGGGTTTAT TGGATACACG GGTACCCGGG CGACTCAGTC
961 AATCGGAGGA CTGGCGCCCC GAGTGAGGGG TTGTGGGCTC TTTTATTGAG CTCGGGGAGC
1021 AGAAGCGCGC GAACAGAAGC GAGAAGCGAA CTGATTGGTT AGTTCAAATA AGGCACAGGG
1081 TCATTTCAGG TCCTTGGGGC ACCCTGGAAA CATCTGATGG TTCTCTAGGG TCGACCCTGT
1141 TCCATCTGTT CCTGACCTTG ATCTGAACTT CTCTATTCTC AGTTATGTAT TTTTCCATGC
1201 CTTGCAAAAT GGCGTTACTT AAGCTAGCTT GCCAAACCTA CAGGTGGGGT CTTTCATTCC
1261 CCCCTTTTTC TGGAGACTAA ATAAAATCTT TTATTTTATC TATGGCTCGT ACTCTATAGG
1321 CTTCAGCTGG TGATATTGTT GAGTCAAAAC TAGAGCCTGG ACCACTGATA TCCTGTCTTT
1381 AACAAATTGG ACTAATCGAA GGTACCCTAT TACACCCACT CGTGCAGGCT GCCCAGGGGC
1441 TTGCCCAGGC TGGTCAGCTG GGCGATGGCG GTCTCGTGCT GCTCCACGAA GCCGCCGTCC
1501 TCCACGTAGG TCTTCTCCAG GCGGTGCTGG ATGAAGTGGT ACTCGGGGAA GTCCTTCACC
1561 ACGCCCTTGC TCTTCATCAG GGTGCGCATG TGGCAGCTGT AGAACTTGCC GCTGTTCAGG
1621 CGGTACACCA GGATCACCTG GCCCACCAGC ACGCCGTCGT TCATGTACAC CACCTCGAAG
1681 CTGGGCTGCA GGCCGGTGAT GGTCTTCTTC ATCACGGGGC CGTCGTTGGG GAAGTTGCGG
1741 CCCTTGTACT CCACGCGGTA CACGAACATC TCCTCGATCA GGTTGATGTC GCTGCGGATC
1801 TCCACCAGGC CGCCGTCCTC GTAGCGCAGG GTGCGCTCGT ACACGAAGCC GGCGGGGAAG
1861 CTCTGGATGA AGAAGTCGCT GATGTCCTCG GGGTACTTGG TGAAGGTGCG GTTGCCGTAC
1921 TGGAAGGCGG GGCTCAGGAT GTCGAAGGCG AAGGGCAGGG GGGCGCCCTT GGTCACGCGG
1981 ATCTGCACCA GCTGGTTGCC GAACAGGATG TTGCCCTTGC CGCAGCCCTC CATGGTGAAC
2041 ACGTGGTTGT TCACCACGCC CTCCAGGTTC ACCTTGAAGC TCATGATCTC CTGCAGGCCG
2101 GTGTTCTTCA GGATCTGCTT GCTCACCATG GTCTCGAGGT CGACGGTATC GATAAGCTTT
2161 TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC
2221 GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG
2281 GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT
2341 GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA
2401 CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT
2461 GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGGG TCGACCACTG TGCTGGCGAA
2521 TTCCATATGG GAGTACTAGT ACGCGTACCG GTGTTTCGTC CTTTCCACAA GATATATAAA
2581 GCCAAGAAAT CGAAATACTT TCAAGTTACG GTAAGCATAT GATAGTCCAT TTTAAAACAT
2641 AATTTTAAAA CTGCAAACTA CCCAAGAAAT TATTACTTTC TACGTCACGT ATTTTGTACT
2701 AATATCTTTG TGTTTGCAGT CAAATTAATT CTAATTATCT CTCTAACAGC CTTGTATCGT
2761 ATATGCAAAT ATGAAGGAAT CATGGGAAAT AGGCCCTCTT CCTGCCCGAC CTTGTCGACA
2821 GATCTTCAAT TCCGGCGCCT AGAGAAGGAG TGAGGGCTGG ATAAAGGGAG GATCGAGGCG
2881 GGGTCGAACG AGGAGGTTCA AGGGGGAGAG ACGGGGCGGA TGGAGGAAGA GGAGGCGGAG
2941 GCTTAGGGTG TACAAAGGGC TTGACCCAGG GAGGGGGGTC AAAAGCCAAG GCTTCCCAGG
3001 TCACGATGTA GGGGACCTGG TCTGGGTGTC CATGCGGGCC AGGTGAAAAG ACCTTGATCT
3061 TAACCTGGGT GATGAGGTCT CGGTTAAAGG TGCCGTCTCG CGGCCATCCG ACGTTAAAGG
3121 TTGGCCATTC TGCAGAGCAG AAGGTAACCC AACGTCTCTT CTTGACATCT ACCGACTGGT
3181 TGTGAGCGAT CCGCTCGACA TCTTTCCAGT GACCTAAGGT CAAACTTAAG GGAGTGGTAA
3241 CAGTCTGGCC CTAATTTTCA GACAAATACA GAAACACAGT CAGACAGAGA CAACACAGAA
3301 CGATGCTGCA GCGCTGCAGC AGACAAGACG CGCGGCTTCG GTTCCAAACC GAAAGCAAAA
3361 ATTCAGACGG AGGCGGGAAC TGTTTTAGGT TCTCGTCTCC TACCAGAACC ACATATCCTG
3421 ACGGGGTCGG ATTCCACATC GACTCCCTTC CTCAGGTCGG GCCACAAAAA CGGCCCCCAA
3481 AGTCCCTGGG ACGTCTCCCA GGGTTGCGGC CGGGTGTTCA GAACTCGTCA GTTCCACCAC
3541 GGGTCCGCCA GATACAGAGC TAGTTAGCTA ACTAGTACAG ACGCAGGCGC ATAACATCAA
3601 ACATAGACAC TAGACAATCG GACAGACACA GATAAGTTGC TGGCCAGCTT ACCTCCCGGT
3661 GGTGGGTCGG TGGTCCCTGG GCAGGGGTCT CCAAATCCCG GACGAGCCCC CAAATGAAAG
3721 ACCCCCGTCG TGGGTAGTCA ATCACTCAGA GGAGACCCTC CCAAGGAACA GCGAGACCAC
3781 GATTCGGATG CAAACAGCAA GAGGCTTTAT TGGGAATACG GGTACCCGGG CGACGCAGTC
3841 TATCGGAAGA CTGGCGCGCC GAGTGAGGGG TTGTGGGCTC TTTTATTGAG CTCTGCTTAT
3901 ATAGACCTCC CACCGTACAC GCCTACCGCC CATTTGCGTC AATGGGGCGG AGTTGTTACG
3961 ACATTTTGGA AAGTCCCGTT GATTTTGGTG CCAAAACAAA CTCCCATTGA CGTCAATGGG
4021 GTGGAGACTT GGAAATCCCC GTGAGTCAAA CCGCTATCCA CGCCCATTGA TGTACTGCCA
4081 AAACCGCATC ACCATGGTAA TAGCGATGAC TAATACGTAG ATGTACTGCC AAGTAGGAAA
4141 GTCCCATAAG GTCATGTACT GGGCATAATG CCAGGCGGGC CATTTACCGT CATTGACGTC
4201 AATAGGGGGC GTACTTGGCA TATGATACAC TTGATGTACT GCCAAGTGGG CAGTTTACCG
4261 TAAATACTCC ACCCATTGAC GTCAATGGAA AGTCCCTATT GGCGTTACTA TGGGAACATA
4321 CGTCATTATT GACGTCAATG GGCGGGGGTC GTTGGGCGGT CAGCCAGGCG GGCCATTTAC
4381 CGTAAGTTAT GTAACGCGGA ACTCCATATA TGGGCTATGA ACTAATGACC CCGTAATTGA
4441 TTACTATTAA TAACTAGTCA ATAATCAATG TCAACATGGC GGTAATGTTG GACATGAGCC
4501 AATATAAATG TACATATTAT GATATGGATA CAACGTATGC AATGGCCAAG CTAATTCTTG
4561 AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT
4621 TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT
4681 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA
4741 ATAATTAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA
4801 AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTAGGTGGCA CTTTTCGGGG
4861 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT
4921 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT
4981 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC
5041 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG
5101 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG
5161 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA
5221 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA
5281 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC
5341 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC
5401 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG
5461 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC
5521 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA
5581 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT
5641 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT
5701 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG
5761 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT
5821 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT
5881 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT
5941 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC
6001 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT
6061 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG
6121 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA
6181 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC
6241 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA
6301 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC
6361 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA
6421 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG
6481 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG
6541 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG
6601 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC
6661 TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC
6721 TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAG//
pMKO.1-GFP其他相关逆病毒载体：
