pBABE-GFP
pBABE-GFP
编号 | 载体名称 |
北京华越洋VECT55124 | pBABE-GFP |
pBABE-GFP载体基本信息：
载体名称: | pBABE-GFP |
质粒类型: | 逆病毒载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶，多克隆位点 |
启动子: | MSCV |
载体大小: | 5183 bp |
5' 测序引物及序列: | pBABE 5' primer 5'CTTTATCCAGCCCTCAC3' |
3' 测序引物及序列: | pBABE 3' primer 5'ACCCTAACTGACACACATTCC3' |
载体标签: | 无 |
载体抗性: | 氨苄青霉素 |
筛选标记: | hrGFP |
克隆菌株: | DH5α、HB101等 |
宿主细胞（系）: | 常规哺乳动物细胞系 |
备注: |
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稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 逆转录病毒 |
pBABE-GFP载体质粒图谱和多克隆位点信息：
pBABE-GFP载体序列：
ORIGIN
1 CTCGTCCGGG ATCGGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG GGAGGCAAGC
61 TGGCCAGCAA CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGACTGA TTTTATGCGC
121 CTGCGTCGGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC
181 GAGTTCTGAA CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGCCGTTTT
241 TGTGGCCCGA CCTGAGGAAG GGAGTCGATG TGGAATCCGA CCCCGTCAGG ATATGTGGTT
301 CTGGTAGGAG ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT
361 TGGAACCGAA GCCGCGCGTC TTGTCTGCTG CAGCGCTGCA GCATCGTTCT GTGTTGTCTC
421 TGTCTGACTG TGTTTCTGTA TTTGTCTGAA AATTAGGGCC AGACTGTTAC CACTCCCTTA
481 AGTTTGACCT TAGGTCACTG GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT
541 GTCAAGAAGA GACGTTGGGT TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA
601 TGGCCGCGAG ACGGCACCTT TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT
661 TCACCTGGCC CGCATGGACA CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG
721 GCTTTTGACC CCCCTCCCTG GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT
781 CCTCCATCCG CCCCGTCTCT CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC
841 CTTTATCCAG CCCTCACTCC TTCTCTAGGC GCCGGCCGGA TCCCAGTGTG GTGGTACGTA
901 GGAATTCGCC AGCACAGTGG TCGACCCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT
961 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA
1021 GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT
1081 AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT
1141 CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG
1201 CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT
1261 GCAAAAAGCT TATCGATACC GTCGACCTCG AGACCATGGT GAGCAAGCAG ATCCTGAAGA
1321 ACACCGGCCT GCAGGAGATC ATGAGCTTCA AGGTGAACCT GGAGGGCGTG GTGAACAACC
1381 ACGTGTTCAC CATGGAGGGC TGCGGCAAGG GCAACATCCT GTTCGGCAAC CAGCTGGTGC
1441 AGATCCGCGT GACCAAGGGC GCCCCCCTGC CCTTCGCCTT CGACATCCTG AGCCCCGCCT
1501 TCCAGTACGG CAACCGCACC TTCACCAAGT ACCCCGAGGA CATCAGCGAC TTCTTCATCC
1561 AGAGCTTCCC CGCCGGCTTC GTGTACGAGC GCACCCTGCG CTACGAGGAC GGCGGCCTGG
1621 TGGAGATCCG CAGCGACATC AACCTGATCG AGGAGATGTT CGTGTACCGC GTGGAGTACA
1681 AGGGCCGCAA CTTCCCCAAC GACGGCCCCG TGATGAAGAA GACCATCACC GGCCTGCAGC
1741 CCAGCTTCGA GGTGGTGTAC ATGAACGACG GCGTGCTGGT GGGCCAGGTG ATCCTGGTGT
1801 ACCGCCTGAA CAGCGGCAAG TTCTACAGCT GCCACATGCG CACCCTGATG AAGAGCAAGG
1861 GCGTGGTGAA GGACTTCCCC GAGTACCACT TCATCCAGCA CCGCCTGGAG AAGACCTACG
1921 TGGAGGACGG CGGCTTCGTG GAGCAGCACG AGACCGCCAT CGCCCAGCTG ACCAGCCTGG
1981 GCAAGCCCCT GGGCAGCCTG CACGAGTGGG TGTAATAGGG TACCTTCGAT AAAATAAAAG
2041 ATTTTATTTA GTCTCCAGAA AAAGGGGGGA ATGAAAGACC CCACCTGTAG GTTTGGCAAG
2101 CTAGCTTAAG TAACGCCATT TTGCAAGGCA TGGAAAAATA CATAACTGAG AATAGAGAAG
2161 TTCAGATCAA GGTCAGGAAC AGATGGAACA GCTGAATATG GGCCAAACAG GATATCTGTG
2221 GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA AGAACAGATG GAACAGCTGA ATATGGGCCA
2281 AACAGGATAT CTGTGGTAAG CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC AGATGGTCCC
2341 CAGATGCGGT CCAGCCCTCA GCAGTTTCTA GAGAACCATC AGATGTTTCC AGGGTGCCCC
2401 AAGGACCTGA AATGACCCTG TGCCTTATTT GAACTAACCA ATCAGTTCGC TTCTCGCTTC
2461 TGTTCGCGCG CTTCTGCTCC CCGAGCTCAA TAAAAGAGCC CACAACCCCT CACTCGGGGC
2521 GCCAGTCCTC CGATTGACTG AGTCGCCCGG GTACCCGTGT ATCCAATAAA CCCTCTTGCA
2581 GTTGCATCCG ACTTGTGGTC TCGCTGTTCC TTGGGAGGGT CTCCTCTGAG TGATTGACTA
2641 CCCGTCAGCG GGGGTCTTTC ACATGCAGCA TGTATCAAAA TTAATTTGGT TTTTTTTCTT
2701 AAGTATTTAC ATTAAATGGC CATAGTTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG
2761 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT
2821 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC
2881 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA
2941 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
3001 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
3061 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
3121 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
3181 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
3241 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
3301 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
3361 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
3421 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
3481 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
3541 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
3601 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
3661 GCGGCCGCAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT
3721 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC
3781 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA
3841 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG
3901 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT
3961 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA
4021 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT
4081 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT
4141 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG
4201 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG
4261 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG
4321 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA
4381 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT
4441 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT
4501 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT
4561 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA
4621 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT
4681 TTCCCCGAAA AGTGCCACCT GCAGCCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC
4741 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGAACAG CTGAATATGG GCCAAACAGG
4801 ATATCTGTGG TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA GAACAGATGG TCCCCAGATG
4861 CGGTCCAGCC CTCAGCAGTT TCTAGAGAAC CATCAGATGT TTCCAGGGTG CCCCAAGGAC
4921 CTGAAATGAC CCTGTGCCTT ATTTGAACTA ACCAATCAGT TCGCTTCTCG CTTCTGTTCG
4981 CGCGCTTCTG CTCCCCGAGC TCAATAAAAG AGCCCACAAC CCCTCACTCG GGGCGCCAGT
5041 CCTCCGATTG ACTGAGTCGC CCGGGTACCC GTGTATCCAA TAAACCCTCT TGCAGTTGCA
5101 TCCGACTTGT GGTCTCGCTG TTCCTTGGGA GGGTCTCCTC TGAGTGATTG ACTACCCGTC
5161 AGCGGGGGTC TTTCATTTGG GGG
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