His镍柱,His蛋白纯化介质填料
His镍柱,His蛋白纯化介质填料
His镍柱,His蛋白纯化介质填料
His镍柱(His蛋白纯化介质填料)产品介绍:
产品英文名称:Ni Sepharose™ 6 Fast Flow - Scale-up Purification 产品标准:5ml体积
产品介绍:
Ni Sepharose™ 6 Fast Flow(GE)具有特异性好、稳定性高、镍离子脱落少、层析介质颗粒粒度均匀、流速快、His标记蛋白结合能量高、使用寿命长、重复性好质量稳定等特点。 His镍柱(His蛋白纯化介质填料) 每ml柱床体积可吸附40mgHis标记蛋白质,可配合注射器或连接液相色谱系统使用。
TECHNICAL SPECIFICATIONS Matrix Highly crossed-linked 6% agarose, precharged with Ni2+ Average particle size 90 µm Dynamic binding capacity* Approx. 40 mg histidine-tagged protein/ml medium Metal ion capacity Approx. 15 µmol Ni2+/ml medium Compatibility during use Stable in all commonly used buffers, reducing agents, denaturants, and detergents, see Introduction to Tagged Protein Purification for more information. Chemical stability For one week at 40°C: 0.01 M HCl, 0.1 M NaOH For 12 h: 1 M NaOH, 70% acetic acid 30 min tested: 30% 2-propanol 1 h tested: 2% SDS pH stability† 2-14 (short term), 3-12 (long term) Storage 20% ethanol Storage temperature 4°C to 30°C * Protein binding capacity is protein-to protein dependent † Ni2+-stripped medium His镍柱(His蛋白纯化介质填料)现货促销。。。。
|