pRS424

pRS424

pRS424载体基本信息：

pRS424载体图谱：

pRS424载体简介：

 Restriction digests of the clone give the following sizes (kb): BamHI--5.6; EcoRI--5.6; PvuII--5.2, 0.5; XbaI--3.3, 1.4, 1.0.Contains the REP3 and FRT sequences necessary for high copy propagation in yeast.In S. cerevisiae, the number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 1.5 + or - 1.7% of progeny per doubling.YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing.The order of the major features in this plasmid is: ori(f1) - lacZ - T7 promoter - MCS (KpnI-SacI) - T3 promoter - lacI - ori(pMB1) - ampR - ori(2 micron) - TRP1.

pRS424载体序列：

ORIGIN

        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

      181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat

      241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa

      301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa

      361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat

      421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta

      481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg

      541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa

      601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa

      661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg

      721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt

      781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag

      841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg

      901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg

      961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa

     1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg

     1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat

     1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga

     1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt

     1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa

     1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca

     1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc

     1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg

     1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg

     1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg

     1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg

     1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga

     1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga

     1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag

     1861 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac

     1921 ggtatcgata agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg

     1981 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt

     2041 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca

     2101 caacatagga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact

     2161 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct

     2221 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc

     2281 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca

     2341 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg

     2401 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca

     2461 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa

     2521 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc

     2581 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc

     2641 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct

     2701 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg

     2761 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag

     2821 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta

     2881 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg

     2941 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt

     3001 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt

     3061 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag

     3121 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat

     3181 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc

     3241 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat

     3301 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc

     3361 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag

     3421 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag

     3481 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt

     3541 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg

     3601 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt

     3661 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc

     3721 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc

     3781 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa

     3841 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg

     3901 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc

     3961 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag

     4021 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt

     4081 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt

     4141 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc

     4201 acctgaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa

     4261 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc

     4321 tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc

     4381 gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag

     4441 agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg

     4501 agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta

     4561 taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac

     4621 tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat

     4681 tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt atattctata

     4741 ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg

     4801 gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt

     4861 ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt

     4921 ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt

     4981 caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa

     5041 agagatactt ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta

     5101 cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa

     5161 agcgctctga agttcctata ctttctagag aataggaact tcggaatagg aacttcaaag

     5221 cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg

     5281 ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca

     5341 tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt

     5401 agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag

     5461 cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca

     5521 atgctatcat ttcctttgat attggatcat attaagaaac cattattatc atgacattaa

     5581 cctataaaaa taggcgtatc acgaggccct ttcgtc

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