pRS423
pRS423
编号 | 载体名称 |
北京华越洋VECT75544 | pRS423 |
pRS423载体基本信息：
启动子: | HIS3,T7,T3 |
复制子: | 2μ ori,ori,FI ori |
质粒分类: | 酵母系列，酿酒酵母表达载体 |
质粒大小: | 5797bp |
原核抗性: | Amp |
筛选标记: | HIS3 |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧 LB |
表达宿主: | 酵母细胞 |
诱导方式: | 半乳糖诱导 |
5'测序引物: | T7:TAATACGACTCACTATAGGG |
3'测序引物: | 根据序列设计引物 |
pRS423载体图谱：
pRS423载体简介：
A YEtype(episomal) E. coli/S. cerevisiae shuttle vector permitting RNA synthesis in vitro, visual detection of recombinants, production of single stranded DNA and containing primer sites useful for sequencing Designation: pRS423
pRS423载体序列：
ORIGIN
1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
61 cttagatgat ccaatatcaa aggaaatgat agcattgaag gatgagacta atccaattga
121 ggagtggcag catatagaac agctaaaggg tagtgctgaa ggaagcatac gataccccgc
181 atggaatggg ataatatcac aggaggtact agactacctt tcatcctaca taaatagacg
241 catataagta cgcatttaag cataaacacg cactatgccg ttcttctcat gtatatatat
301 atacaggcaa cacgcagata taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg
361 cgttgcattt tcggaagcgc tcgttttcgg aaacgctttg aagttcctat tccgaagttc
421 ctattctcta gaaagtatag gaacttcaga gcgcttttga aaaccaaaag cgctctgaag
481 acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa atattgcgaa
541 taccgcttcc acaaacattg ctcaaaagta tctctttgct atatatctct gtgctatatc
601 cctatataac ctacccatcc acctttcgct ccttgaactt gcatctaaac tcgacctcta
661 cattttttat gtttatctct agtattactc tttagacaaa aaaattgtag taagaactat
721 tcatagagtg aatcgaaaac aatacgaaaa tgtaaacatt tcctatacgt agtatataga
781 gacaaaatag aagaaaccgt tcataatttt ctgaccaatg aagaatcatc aacgctatca
841 ctttctgttc acaaagtatg cgcaatccac atcggtatag aatataatcg gggatgcctt
901 tatcttgaaa aaatgcaccc gcagcttcgc tagtaatcag taaacgcggg aagtggagtc
961 aggctttttt tatggaagag aaaatagaca ccaaagtagc cttcttctaa ccttaacgga
1021 cctacagtgc aaaaagttat caagagactg cattatagag cgcacaaagg agaaaaaaag
1081 taatctaaga tgctttgtta gaaaaatagc gctctcggga tgcatttttg tagaacaaaa
1141 aagaagtata gattctttgt tggtaaaata gcgctctcgc gttgcatttc tgttctgtaa
1201 aaatgcagct cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgtttta
1261 caaaaatgaa gcacagattc ttcgttggta aaatagcgct ttcgcgttgc atttctgttc
1321 tgtaaaaatg cagctcagat tctttgtttg aaaaattagc gctctcgcgt tgcatttttg
1381 ttctacaaaa tgaagcacag atgcttcgtt caggtggcac ttttcgggga aatgtgcgcg
1441 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
1501 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
1561 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
1621 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
1681 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
1741 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
1801 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
1861 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
1921 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
1981 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
2041 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
2101 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
2161 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
2221 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
2281 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
2341 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
2401 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
2461 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
2521 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
2581 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
2641 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
2701 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
2761 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
2821 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
2881 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
2941 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
3001 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
3061 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
3121 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
3181 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
3241 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
3301 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
3361 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
3421 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta cctcactcat taggcacccc
3481 aggctttaca ctttatgctt ccggctccta tgttgtgtgg aattgtgagc ggataacaat
3541 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
3601 gggaacaaaa gctggagctc caccgcggtg gcggccgctc tagaactagt ggatcccccg
3661 ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta
3721 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
3781 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
3841 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
3901 ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
3961 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
4021 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
4081 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
4141 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
4201 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
4261 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
4321 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcctga
4381 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatagat ccgtcgagtt
4441 caagagaaaa aaaaagaaaa agcaaaaaga aaaaaggaaa gcgcgcctcg ttcagaatga
4501 cacgtataga atgatgcatt accttgtcat cttcagtatc atactgttcg tatacatact
4561 tactgacatt cataggtata catatataca catgtatata tatcgtatgc tgcagcttta
4621 aataatcggt gtcactacat aagaacacct ttggtggagg gaacatcgtt ggtaccattg
4681 ggcgaggtgg cttctcttat ggcaaccgca agagccttga acgcactctc actacggtga
4741 tgatcattct tgcctcgcag acaatcaacg tggagggtaa ttctgctagc ctctgcaaag
4801 ctttcaagaa aatgcgggat catctcgcaa gagagatctc ctactttctc cctttgcaaa
4861 ccaagttcga caactgcgta cggcctgttc gaaagatcta ccaccgctct ggaaagtgcc
4921 tcatccaaag gcgcaaatcc tgatccaaac ctttttactc cacgcgccag tagggcctct
4981 ttaaaagctt gaccgagagc aatcccgcag tcttcagtgg tgtgatggtc gtctatgtgt
5041 aagtcaccaa tgcactcaac gattagcgac cagccggaat gcttggccag agcatgtatc
5101 atatggtcca gaaaccctat acctgtgtgg acgttaatca cttgcgattg tgtggcctgt
5161 tctgctactg cttctgcctc tttttctggg aagatcgagt gctctatcgc taggggacca
5221 ccctttaaag agatcgcaat ctgaatcttg gtttcatttg taatacgctt tactagggct
5281 ttctgctctg tcatctttgc cttcgtttat cttgcctgct cattttttag tatattcttc
5341 gaagaaatca cattacttta tataatgtat aattcattat gtgataatgc caatcgctaa
5401 gaaaaaaaaa gagtcatccg ctaggggaaa aaaaaaaatg aaaatcatta ccgaggcata
5461 aaaaaatata gagtgtacta gaggaggcca agagtaatag aaaaagaaaa ttgcgggaaa
5521 ggactgtgtt atgacttccc tgactaatgc cgtgttcaaa cgatacctgg cagtgactcc
5581 tagcgctcac caagctctta aaacgggaat ttatggtgca ctctcagtac aatctgctct
5641 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
5701 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
5761 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcga
//
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