pGAPZαB
pGAPZαB
编号 | 载体名称 |
北京华越洋VECT75525 | pGAPZαB |
pGAPZαB载体基本信息:
启动子: | GAP |
复制子: | pUC ori |
终止子: | AOX1 TT |
质粒分类: | 酵母系列,毕赤酵母表达载体 |
质粒大小: | 3151bp |
质粒标签: | C-Myc, C-6×His |
原核抗性: | Zeo |
筛选标记: | HIS4 |
克隆菌株: | DH5α |
培养条件: | 37℃,有氧 LB |
表达宿主: | 酵母细胞 |
5'测序引物: | pGAP-F:GTCCCTATTTCAATCAATTGAA |
3'测序引物: | AOX3:GGCAAATGGCATTCTGACAT |
pGAPZαB载体图谱:
pGAPZαB载体简介:
甘油醛-3-磷酸脱氢酶(GAPDH)的酶在许多生物细胞中包括毕赤酵母,稳定高水平表达。最近GAPDH蛋白编码基因启动子(GAP)被深入研究,并在毕赤酵母中能够高水表达重组蛋白,这取决于使用的碳源(Waterham等 人,,1997)。GAP启动子(PGAP)的重组蛋白表达水平略高于AOX1启动子。
pGAPZ A,B,和C载体(2.9 KB)和pGAPZαB和C(3.1 KB)载体使用GAP启动子在毕赤酵母中稳定表达重组蛋白。重组蛋白表达为为带有C-末端myc抗原表位和C端His标签的融合蛋白。此外,pGAPZα 表达的融合蛋白在N-端带有酿酒酵母(Saccharomyces cerevisiae)的α-因子分泌信号肽。这两个系列的载体都提供三个不同读码框的载体,以方便克隆的带有C-端标签和/或N-末端分泌信号肽的载体中。
pGAPZαB载体序列:
ORIGIN
1 agatcttttt tgtagaaatg tcttggtgtc ctcgtccaat caggtagcca tctctgaaat
61 atctggctcc gttgcaactc cgaacgacct gctggcaacg taaaattctc cggggtaaaa
121 cttaaatgtg gagtaatgga accagaaacg tctcttccct tctctctcct tccaccgccc
181 gttaccgtcc ctaggaaatt ttactctgct ggagagcttc ttctacggcc cccttgcagc
241 aatgctcttc ccagcattac gttgcgggta aaacggaggt cgtgtacccg acctagcagc
301 ccagggatgg aaaagtcccg gccgtcgctg gcaataatag cgggcggacg catgtcatga
361 gattattgga aaccaccaga atcgaatata aaaggcgaac acctttccca attttggttt
421 ctcctgaccc aaagacttta aatttaattt atttgtccct atttcaatca attgaacaac
481 tatttcgaaa cgatgagatt tccttcaatt tttactgctg ttttattcgc agcatcctcc
541 gcattagctg ctccagtcaa cactacaaca gaagatgaaa cggcacaaat tccggctgaa
601 gctgtcatcg gttactcaga tttagaaggg gatttcgatg ttgctgtttt gccattttcc
661 aacagcacaa ataacgggtt attgtttata aatactacta ttgccagcat tgctgctaaa
721 gaagaagggg tatctctcga gaaaagagag gctgaagctg caggaattca cgtggcccag
781 ccggccgtct cggatcggta cctcgagccg cggcggccgc cagctttcta gaacaaaaac
841 tcatctcaga agaggatctg aatagcgccg tcgaccatca tcatcatcat cattgagttt
901 tagccttaga catgactgtt cctcagttca agttgggcac ttacgagaag accggtcttg
961 ctagattcta atcaagagga tgtcagaatg ccatttgcct gagagatgca ggcttcattt
1021 ttgatacttt tttatttgta acctatatag tataggattt tttttgtcat tttgtttctt
1081 ctcgtacgag cttgctcctg atcagcctat ctcgcagctg atgaatatct tgtggtaggg
1141 gtttgggaaa atcattcgag tttgatgttt ttcttggtat ttcccactcc tcttcagagt
1201 acagaagatt aagtgagacc ttcgtttgtg cggatccccc acacaccata gcttcaaaat
1261 gtttctactc cttttttact cttccagatt ttctcggact ccgcgcatcg ccgtaccact
1321 tcaaaacacc caagcacagc atactaaatt ttccctcttt cttcctctag ggtgtcgtta
1381 attacccgta ctaaaggttt ggaaaagaaa aaagagaccg cctcgtttct ttttcttcgt
1441 cgaaaaaggc aataaaaatt tttatcacgt ttctttttct tgaaattttt ttttttagtt
1501 tttttctctt tcagtgacct ccattgatat ttaagttaat aaacggtctt caatttctca
1561 agtttcagtt tcatttttct tgttctatta caactttttt tacttcttgt tcattagaaa
1621 gaaagcatag caatctaatc taagggcggt gttgacaatt aatcatcggc atagtatatc
1681 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
1741 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
1801 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
1861 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
1921 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
1981 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
2041 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtccga
2101 cggcggccca cgggtcccag gcctcggaga tccgtccccc ttttcctttg tcgatatcat
2161 gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc taaccgaaaa
2221 ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt
2281 attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc
2341 atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat
2401 ttgcaagctg gagaccaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
2461 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
2521 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
2581 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
2641 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
2701 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
2761 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
2821 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
2881 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
2941 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
3001 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
3061 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
3121 acgttaaggg attttggtca tgcatgagat c
//
pGAPZαB载体其他相关的毕赤酵母过表达载体:
pYM39-EYFP |
pYM33-EBFP |
pYM30-ECFP |
pPICZαC |
pPICZαB |
pPICZαA |
pPICZC |
pPICZB |
pPICZA |
pGAPZαC |
pGAPZαB |
PGAPZαA |
pGAPZC |
pGAPZB |
pGAPZA |