北京华越洋生物
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首页    载体及质粒    pENTR2B
pENTR2BMap

pENTR2B

pENTR2B

pENTR2B

 



编号

载体名称

北京华越洋VECT511194

pENTR2B

 

pENTR2B载体基本信息:

载体名称:

pENTR2B

质粒类型:

Gateway载体;入门载体

高拷贝/低拷贝:

高拷贝

启动子:

克隆方法:

限制性内切酶;多克隆位点

载体大小:

2718 bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

载体抗性:

卡那霉素

真核筛选标记:

备注:

 

pENTR2B载体本身含有CCDb致死基因,能够使得普通的DH5alpha等细菌死亡,必须使用DB3.1感受态细胞进行质粒扩增;
 
 
构建Gateway系统入门克隆时需要使用ccdB敏感的 OmniMAX2感受态细胞。
   

病毒/非病毒:

非病毒

 

pENTR2B载体质粒图谱和多克隆位点信息:

 

 

pENTR2B载体简介

pENTR2B载体序列:

ORIGIN

    1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCA TGGATCTCGG

   61 GGACGTCTAA CTACTAAGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT

  121 CAGTCGGAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT

  181 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGTGAAGC AACGGCCCGG AGGGTGGCGG

  241 GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAACTAAGC AGAAGGCCAT CCTGACGGAT

  301 GGCCTTTTTG CGTTTCTACA AACTCTTCCT GTTAGTTAGT TACTTAAGCT CGGGCCCCAA

  361 ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA TAAGCAATGC

  421 TTTTTTATAA TGCCAACTTT GTACAAAAAA GCAGGCTGGC GCCGGAACCA ATTCAGTCGA

  481 CTGGATCCGG TACCGAATTC GCTTACTAAA AGCCAGATAA CAGTATGCGT ATTTGCGCGC

  541 TGATTTTTGC GGTATAAGAA TATATACTGA TATGTATACC CGAAGTATGT CAAAAAGAGG

  601 TGTGCTTCTA GAATGCAGTT TAAGGTTTAC ACCTATAAAA GAGAGAGCCG TTATCGTCTG

  661 TTTGTGGATG TACAGAGTGA TATTATTGAC ACGCCCGGGC GACGGATGGT GATCCCCCTG

  721 GCCAGTGCAC GTCTGCTGTC AGATAAAGTC TCCCGTGAAC TTTACCCGGT GGTGCATATC

  781 GGGGATGAAA GCTGGCGCAT GATGACCACC GATATGGCCA GTGTGCCGGT CTCCGTTATC

  841 GGGGAAGAAG TGGCTGATCT CAGCCACCGC GAAAATGACA TCAAAAACGC CATTAACCTG

  901 ATGTTCTGGG GAATATAGAA TTCGCGGCCG CACTCGAGAT ATCTAGACCC AGCTTTCTTG

  961 TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT

 1021 ATCAGTCAAA ATAAAATCAT TATTTGCCAT CCAGCTGCAG CTCTGGCCCG TGTCTCAAAA

 1081 TCTCTGATGT TACATTGCAC AAGATAAAAA TATATCATCA TGAACAATAA AACTGTCTGC

 1141 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC

 1201 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT

 1261 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT

 1321 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA

 1381 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA

 1441 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA

 1501 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC

 1561 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA

 1621 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG

 1681 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT

 1741 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG

 1801 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG

 1861 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT

 1921 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC

 1981 AGAATTGGTT AATTGGTTGT AACATTATTC AGATTGGGCC CCGTTCCACT GAGCGTCAGA

 2041 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG

 2101 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC

 2161 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT

 2221 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC

 2281 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT

 2341 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG

 2401 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT

 2461 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG

 2521 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG

 2581 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG

 2641 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG

 2701 GCCTTTTGCT CACATGTT

//

 

pENTR2B其他相关Gateway载体:

pLKO-DEST-puro

pcDNA6.2/V5-PL-DEST

pLenti7.3-V5-DEST

pcDNA6.2/N-YFP-DEST

pLenti6-UbC-V5-DEST

pcDNA3.2/V5-DEST

pLenti4-V5-DEST

pAd/BLOCK-iT-DEST

pLenti4-BLOCK-iT     -DEST

pT-Rex-DEST30

pIB-V5-His-DEST

pLKO-DEST-EGFP

pET-DEST42

plenti6/V5-Dest

pENTR-MCS1-IRES2-EGFP

pLenti6-tdTomato-V5-DEST

pENTR-L5-pLac-Spec-L2

pLenti6/BLOCK-iT     -DEST

pENTR-GUS

pLenti6.4/R4R2/V5-DEST

pENTR-DsRed2-MCS1     miR

pLenti6.2/V5-DEST

pENTR221

pENTR-L1-pLac-lacZa-R5

pENTR3C-Dual

pENTR11-Dual

pENTR     H1 TO

pEXP4-DEST

pDONR222

pEXP3-DEST

pDONR221-P4RP3R

pENTR5'/EF1αp

pDONR     Zeo

pEXP2-DEST

pDest40

pENTR4-FLAG

pDEST24

pENTR4

pDEST22

pENTR2B

pDEST10

pEXP1-DEST

pcDNA-DEST53

pDONR-P2RP3

pcDNA6.2-N-EmGFP-DEST

pDONR221-P1P4

pAd-BLOCK-iT-DEST

pDONR221

pLKO-DEST-YFP

pDEST26

pLenti6.2/C-Lumio/V5-DEST

pDEST15

pLenti6/TR

pcDNA-DEST40

pLenti4/TO/V5-DEST

pcDNA6/BioEase-DEST

pLenti4/V5-GW/LacZ

pcDNA6.2/GW/EmGFP-miR     negative

pENTR-MCS1-IRES2-DsRed2

pcDNA6.2/C-EmGFP-DEST

pENTR-L5-LacI-L4

pAd/CMV/V5-DEST

pENTR-L1pLaclacZaL4

pT-Rex-DEST31

pENTR11-Dual

pMT-DEST48

pENTR5'/CMVp

pLKO-DEST-hygro

pENTR4-Dual

plenti-CMV-puro-DEST

pENTR1A

pLenti6-capTEV-CT-DEST

pEF-DEST51

pLenti6.3-V5-DEST

pDONR223

pENTR-R4-pLac-Spec-R3

pDONR221-P5P4

pENTR-GeneBLAzer

pDONR221-P5P2

pENTR5-UbCp

pDONR201

pENTR3C

pDEST20

pENTR     U6

pDEST17

pENTR     2B Dual

pDEST12.2

pEBNA-DEST

pDONR-P4P1R

pET301/CT-DEST

pDONR207

pET300/NT-DEST

pDEST27

pcDNA6.2/cGeneBLAzer-DEST

pDEST14

pCMV-SPORT6

pDEST8

pCMV-SPORT6

pcDNA-DEST47

pcDNA6.2/nGeneBLAzer-DEST

pcDNA6.2-V5-DEST

pcDNA6.2/C-YFP-DEST

pcDNA3.1-nV5-DEST

pcDNA6.2/cTC-Tag-DEST

pBAD-DEST49

pcDNA6.2/cLumio-DEST

pAd-PL-DEST

pcDNA6.2/nLumio-DEST

pMT/BioEase-DEST

pDEST32

pXINSECT-DEST38

pYES-DEST52

pXINSECT-DEST39