北京华越洋生物
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首页    载体及质粒    pENTR4
pENTR4Map

pENTR4

pENTR4

pENTR4

 



编号

载体名称

北京华越洋VECT511192

pENTR4

 

pENTR4载体基本信息:

载体名称:

pENTR4

质粒类型:

Gateway载体;入门载体

高拷贝/低拷贝:

高拷贝

启动子:

克隆方法:

限制性内切酶;多克隆位点

载体大小:

2720 bp

5' 测序引物及序列:

pENTR-F: CTACAAACTCTTCCTGTTAGTTAG

3' 测序引物及序列:

pENTR-R:   ATGGCTCATAACACCCCTTG

载体标签:

载体抗性:

卡那霉素

真核筛选标记:

备注:


  pENTR4
载体本身含有CCDb致死基因,能够使得普通的DH5alpha等细菌死亡,必须使用DB3.1感受态细胞进行质粒扩增;
 
 
构建Gateway系统入门克隆时需要使用ccdB敏感的 OmniMAX2感受态细胞。
   

病毒/非病毒:

非病毒

 

pENTR4载体质粒图谱和多克隆位点信息:

 

 

pENTR4载体简介

pENTR4载体序列:

ORIGIN

        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCA TGGATCTCGG

       61 GGACGTCTAA CTACTAAGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT

      121 CAGTCGGAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT

      181 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGTGAAGC AACGGCCCGG AGGGTGGCGG

      241 GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAACTAAGC AGAAGGCCAT CCTGACGGAT

      301 GGCCTTTTTG CGTTTCTACA AACTCTTCCT GTTAGTTAGT TACTTAAGCT CGGGCCCCAA

      361 ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA TAAGCAATGC

      421 TTTTTTATAA TGCCAACTTT GTACAAAAAA GCAGGCTCCA CCATGGGAAC CAATTCAGTC

      481 GACTGGATCC GGTACCGAAT TCGCTTACTA AAAGCCAGAT AACAGTATGC GTATTTGCGC

      541 GCTGATTTTT GCGGTATAAG AATATATACT GATATGTATA CCCGAAGTAT GTCAAAAAGA

      601 GGTGTGCTTC TAGAATGCAG TTTAAGGTTT ACACCTATAA AAGAGAGAGC CGTTATCGTC

      661 TGTTTGTGGA TGTACAGAGT GATATTATTG ACACGCCCGG GCGACGGATG GTGATCCCCC

      721 TGGCCAGTGC ACGTCTGCTG TCAGATAAAG TCTCCCGTGA ACTTTACCCG GTGGTGCATA

      781 TCGGGGATGA AAGCTGGCGC ATGATGACCA CCGATATGGC CAGTGTGCCG GTCTCCGTTA

      841 TCGGGGAAGA AGTGGCTGAT CTCAGCCACC GCGAAAATGA CATCAAAAAC GCCATTAACC

      901 TGATGTTCTG GGGAATATAG AATTCGCGGC CGCACTCGAG ATATCTAGAC CCAGCTTTCT

      961 TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA

     1021 CTATCAGTCA AAATAAAATC ATTATTTGCC ATCCAGCTGC AGCTCTGGCC CGTGTCTCAA

     1081 AATCTCTGAT GTTACATTGC ACAAGATAAA AATATATCAT CATGAACAAT AAAACTGTCT

     1141 GCTTACATAA ACAGTAATAC AAGGGGTGTT ATGAGCCATA TTCAACGGGA AACGTCGAGG

     1201 CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT

     1261 GTCGGGCAAT CAGGTGCGAC AATCTATCGC TTGTATGGGA AGCCCGATGC GCCAGAGTTG

     1321 TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA

     1381 AACTGGCTGA CGGAATTTAT GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGGT

     1441 GATGCATGGT TACTCACCAC TGCGATCCCC GGAAAAACAG CATTCCAGGT ATTAGAAGAA

     1501 TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT

     1561 TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG

     1621 CAATCACGAA TGAATAACGG TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC

     1681 TGGCCTGTTG AACAAGTCTG GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA

     1741 GTCGTCACTC ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA

     1801 GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA

     1861 TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT

     1921 ATTGATAATC CTGATATGAA TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA

     1981 TCAGAATTGG TTAATTGGTT GTAACATTAT TCAGATTGGG CCCCGTTCCA CTGAGCGTCA

     2041 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC

     2101 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA

     2161 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT

     2221 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC

     2281 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG

     2341 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG

     2401 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG

     2461 CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC

     2521 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT

     2581 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG

     2641 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC

     2701 TGGCCTTTTG CTCACATGTT

//

 

pENTR4其他相关Gateway载体:

pLKO-DEST-puro

pcDNA6.2/V5-PL-DEST

pLenti7.3-V5-DEST

pcDNA6.2/N-YFP-DEST

pLenti6-UbC-V5-DEST

pcDNA3.2/V5-DEST

pLenti4-V5-DEST

pAd/BLOCK-iT-DEST

pLenti4-BLOCK-iT     -DEST

pT-Rex-DEST30

pIB-V5-His-DEST

pLKO-DEST-EGFP

pET-DEST42

plenti6/V5-Dest

pENTR-MCS1-IRES2-EGFP

pLenti6-tdTomato-V5-DEST

pENTR-L5-pLac-Spec-L2

pLenti6/BLOCK-iT     -DEST

pENTR-GUS

pLenti6.4/R4R2/V5-DEST

pENTR-DsRed2-MCS1     miR

pLenti6.2/V5-DEST

pENTR221

pENTR-L1-pLac-lacZa-R5

pENTR3C-Dual

pENTR11-Dual

pENTR     H1 TO

pEXP4-DEST

pDONR222

pEXP3-DEST

pDONR221-P4RP3R

pENTR5'/EF1αp

pDONR     Zeo

pEXP2-DEST

pDest40

pENTR4-FLAG

pDEST24

pENTR4

pDEST22

pENTR2B

pDEST10

pEXP1-DEST

pcDNA-DEST53

pDONR-P2RP3

pcDNA6.2-N-EmGFP-DEST

pDONR221-P1P4

pAd-BLOCK-iT-DEST

pDONR221

pLKO-DEST-YFP

pDEST26

pLenti6.2/C-Lumio/V5-DEST

pDEST15

pLenti6/TR

pcDNA-DEST40

pLenti4/TO/V5-DEST

pcDNA6/BioEase-DEST

pLenti4/V5-GW/LacZ

pcDNA6.2/GW/EmGFP-miR     negative

pENTR-MCS1-IRES2-DsRed2

pcDNA6.2/C-EmGFP-DEST

pENTR-L5-LacI-L4

pAd/CMV/V5-DEST

pENTR-L1pLaclacZaL4

pT-Rex-DEST31

pENTR11-Dual

pMT-DEST48

pENTR5'/CMVp

pLKO-DEST-hygro

pENTR4-Dual

plenti-CMV-puro-DEST

pENTR1A

pLenti6-capTEV-CT-DEST

pEF-DEST51

pLenti6.3-V5-DEST

pDONR223

pENTR-R4-pLac-Spec-R3

pDONR221-P5P4

pENTR-GeneBLAzer

pDONR221-P5P2

pENTR5-UbCp

pDONR201

pENTR3C

pDEST20

pENTR     U6

pDEST17

pENTR     2B Dual

pDEST12.2

pEBNA-DEST

pDONR-P4P1R

pET301/CT-DEST

pDONR207

pET300/NT-DEST

pDEST27

pcDNA6.2/cGeneBLAzer-DEST

pDEST14

pCMV-SPORT6

pDEST8

pCMV-SPORT6

pcDNA-DEST47

pcDNA6.2/nGeneBLAzer-DEST

pcDNA6.2-V5-DEST

pcDNA6.2/C-YFP-DEST

pcDNA3.1-nV5-DEST

pcDNA6.2/cTC-Tag-DEST

pBAD-DEST49

pcDNA6.2/cLumio-DEST

pAd-PL-DEST

pcDNA6.2/nLumio-DEST

pMT/BioEase-DEST

pDEST32

pXINSECT-DEST38

pYES-DEST52

pXINSECT-DEST39