pX602
pX602
编号 | 载体名称 |
北京华越洋VECT75269 | pX602 |
pX602 载体基本信息：
载体名称: | pX602 或 pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS- bGHpA;U6::BsaI-sgRNA 或 pX602-TBG-SaCas9(reopt)-OLLAS-sgRNA-U6(BsaI) |
质粒类型: | CRISPR-Cas9载体；基因敲除载体；哺乳动物载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶，多克隆位点 |
启动子: | U6 |
载体大小: | 7923 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | BGH rev:TAGAAGGCACAGTCGAGG |
载体标签: | NLS (N ter)；NLS、HA、OLLAS tag (C ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | Stbl3 |
宿主细胞（系）: | 常规哺乳动物细胞等 |
备注: | A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA. |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pX602 载体质粒图谱和多克隆位点信息：
pX602 载体序列：
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggcctcta gactcgaggg gctggaagct acctttgaca tcatttcctc
181 tgcgaatgca tgtataattt ctacagaacc tattagaaag gatcacccag cctctgcttt
241 tgtacaactt tcccttaaaa aactgccaat tccactgctg tttggcccaa tagtgagaac
301 tttttcctgc tgcctcttgg tgcttttgcc tatggcccct attctgcctg ctgaagacac
361 tcttgccagc atggacttaa acccctccag ctctgacaat cctctttctc ttttgtttta
421 catgaagggt ctggcagcca aagcaatcac tcaaagttca aaccttatca ttttttgctt
481 tgttcctctt ggccttggtt ttgtacatca gctttgaaaa taccatccca gggttaatgc
541 tggggttaat ttataactaa gagtgctcta gttttgcaat acaggacatg ctataaaaat
601 ggaaagatac cggtgccacc atggccccaa agaagaagcg gaaggtcggt atccacggag
661 tcccagcagc caagcggaac tacatcctgg gcctggacat cggcatcacc agcgtgggct
721 acggcatcat cgactacgag acacgggacg tgatcgatgc cggcgtgcgg ctgttcaaag
781 aggccaacgt ggaaaacaac gagggcaggc ggagcaagag aggcgccaga aggctgaagc
841 ggcggaggcg gcatagaatc cagagagtga agaagctgct gttcgactac aacctgctga
901 ccgaccacag cgagctgagc ggcatcaacc cctacgaggc cagagtgaag ggcctgagcc
961 agaagctgag cgaggaagag ttctctgccg ccctgctgca cctggccaag agaagaggcg
1021 tgcacaacgt gaacgaggtg gaagaggaca ccggcaacga gctgtccacc agagagcaga
1081 tcagccggaa cagcaaggcc ctggaagaga aatacgtggc cgaactgcag ctggaacggc
1141 tgaagaaaga cggcgaagtg cggggcagca tcaacagatt caagaccagc gactacgtga
1201 aagaagccaa acagctgctg aaggtgcaga aggcctacca ccagctggac cagagcttca
1261 tcgacaccta catcgacctg ctggaaaccc ggcggaccta ctatgaggga cctggcgagg
1321 gcagcccctt cggctggaag gacatcaaag aatggtacga gatgctgatg ggccactgca
1381 cctacttccc cgaggaactg cggagcgtga agtacgccta caacgccgac ctgtacaacg
1441 ccctgaacga cctgaacaat ctcgtgatca ccagggacga gaacgagaag ctggaatatt
1501 acgagaagtt ccagatcatc gagaacgtgt tcaagcagaa gaagaagccc accctgaagc
1561 agatcgccaa agaaatcctc gtgaacgaag aggatattaa gggctacaga gtgaccagca
1621 ccggcaagcc cgagttcacc aacctgaagg tgtaccacga catcaaggac attaccgccc
1681 ggaaagagat tattgagaac gccgagctgc tggatcagat tgccaagatc ctgaccatct
1741 accagagcag cgaggacatc caggaagaac tgaccaatct gaactccgag ctgacccagg
1801 aagagatcga gcagatctct aatctgaagg gctataccgg cacccacaac ctgagcctga
1861 aggccatcaa cctgatcctg gacgagctgt ggcacaccaa cgacaaccag atcgctatct
1921 tcaaccggct gaagctggtg cccaagaagg tggacctgtc ccagcagaaa gagatcccca
1981 ccaccctggt ggacgacttc atcctgagcc ccgtcgtgaa gagaagcttc atccagagca
2041 tcaaagtgat caacgccatc atcaagaagt acggcctgcc caacgacatc attatcgagc
2101 tggcccgcga gaagaactcc aaggacgccc agaaaatgat caacgagatg cagaagcgga
2161 accggcagac caacgagcgg atcgaggaaa tcatccggac caccggcaaa gagaacgcca
2221 agtacctgat cgagaagatc aagctgcacg acatgcagga aggcaagtgc ctgtacagcc
2281 tggaagccat ccctctggaa gatctgctga acaacccctt caactatgag gtggaccaca
2341 tcatccccag aagcgtgtcc ttcgacaaca gcttcaacaa caaggtgctc gtgaagcagg
2401 aagaaaacag caagaagggc aaccggaccc cattccagta cctgagcagc agcgacagca
2461 agatcagcta cgaaaccttc aagaagcaca tcctgaatct ggccaagggc aagggcagaa
2521 tcagcaagac caagaaagag tatctgctgg aagaacggga catcaacagg ttctccgtgc
2581 agaaagactt catcaaccgg aacctggtgg ataccagata cgccaccaga ggcctgatga
2641 acctgctgcg gagctacttc agagtgaaca acctggacgt gaaagtgaag tccatcaatg
2701 gcggcttcac cagctttctg cggcggaagt ggaagtttaa gaaagagcgg aacaaggggt
2761 acaagcacca cgccgaggac gccctgatca ttgccaacgc cgatttcatc ttcaaagagt
2821 ggaagaaact ggacaaggcc aaaaaagtga tggaaaacca gatgttcgag gaaaagcagg
2881 ccgagagcat gcccgagatc gaaaccgagc aggagtacaa agagatcttc atcacccccc
2941 accagatcaa gcacattaag gacttcaagg actacaagta cagccaccgg gtggacaaga
3001 agcctaatag agagctgatt aacgacaccc tgtactccac ccggaaggac gacaagggca
3061 acaccctgat cgtgaacaat ctgaacggcc tgtacgacaa ggacaatgac aagctgaaaa
3121 agctgatcaa caagagcccc gaaaagctgc tgatgtacca ccacgacccc cagacctacc
3181 agaaactgaa gctgattatg gaacagtacg gcgacgagaa gaatcccctg tacaagtact
3241 acgaggaaac cgggaactac ctgaccaagt actccaaaaa ggacaacggc cccgtgatca
3301 agaagattaa gtattacggc aacaaactga acgcccatct ggacatcacc gacgactacc
3361 ccaacagcag aaacaaggtc gtgaagctgt ccctgaagcc ctacagattc gacgtgtacc
3421 tggacaatgg cgtgtacaag ttcgtgaccg tgaagaatct ggatgtgatc aaaaaagaaa
3481 actactacga agtgaatagc aagtgctatg aggaagctaa gaagctgaag aagatcagca
3541 accaggccga gtttatcgcc tccttctaca acaacgatct gatcaagatc aacggcgagc
3601 tgtatagagt gatcggcgtg aacaacgacc tgctgaaccg gatcgaagtg aacatgatcg
3661 acatcaccta ccgcgagtac ctggaaaaca tgaacgacaa gaggcccccc aggatcatta
3721 agacaatcgc ctccaagacc cagagcatta agaagtacag cacagacatt ctgggcaacc
3781 tgtatgaagt gaaatctaag aagcaccctc agatcatcaa aaagggcaaa aggccggcgg
3841 ccacgaaaaa ggccggccag gcaaaaaaga aaaagggatc ctacccatac gatgttccag
3901 attacgctag cggcttcgcc aacgagcttg gacccaggtt gatgggaaag taagaattcc
3961 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
4021 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
4081 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
4141 gcaggacagc aagggggagg attgggaaga gaatagcagg catgctgggg aggtaccaaa
4201 aatctcgcca acaagttgac gagataaaca cggcattttg ccttgtttta gtagattctg
4261 tttccagagt actaaaactg agacctgccg tggtctccgg tgtttcgtcc tttccacaag
4321 atatataaag ccaagaaatc gaaatacttt caagttacgg taagcatatg atagtccatt
4381 ttaaaacata attttaaaac tgcaaactac ccaagaaatt attactttct acgtcacgta
4441 ttttgtacta atatctttgt gtttacagtc aaattaattc caattatctc tctaacagcc
4501 ttgtatcgta tatgcaaata tgaaggaatc atgggaaata ggccctcgcg gccgcaggaa
4561 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
4621 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
4681 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
4741 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
4801 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
4861 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
4921 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
4981 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
5041 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
5101 accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt
5161 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
5221 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
5281 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
5341 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
5401 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
5461 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
5521 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
5581 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
5641 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
5701 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
5761 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
5821 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
5881 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
5941 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
6001 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
6061 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
6121 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
6181 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
6241 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
6301 ctgataaatc tggagccggt gagcgtggaa gccgcggtat cattgcagca ctggggccag
6361 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
6421 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
6481 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
6541 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
6601 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
6661 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
6721 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
6781 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
6841 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
6901 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
6961 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
7021 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
7081 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
7141 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
7201 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
7261 tcctggcctt ttgctggcct tttgctcaca tgt
//
pX602 载体其他相关CRISPR/Cas9载体：
