pX601
pX601
编号 | 载体名称 |
北京华越洋VECT75268 | pX601 |
pX601载体基本信息：
载体名称: | pX601 ; pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA 或 pX601-CMV-SaCas9(reopt)-3xHA-U6-BsaI(sgRNA) |
质粒类型: | CRISPR-Cas9载体；基因敲除载体；哺乳动物载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶，多克隆位点 |
启动子: | U6 |
载体大小: | 7446 bp |
5' 测序引物及序列: | CMV fwd:CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | BGH rev:TAGAAGGCACAGTCGAGG |
载体标签: | NLS (N ter)；NLS、3xHA (C ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | Stbl3 |
宿主细胞（系）: | 常规哺乳动物细胞等 |
备注: | SaCas9 is form Staphylococcus aureus。 |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pX601 载体质粒图谱和多克隆位点信息：
pX601 载体序列：
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggcctcta gactcgaggc gttgacattg attattgact agttattaat
181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
541 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
661 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
721 tctatataag cagagctctc tggctaacta ccggtgccac cATGGCCCCA AAGAAGAAGC
781 GGAAGGTCGG TATCCACGGA GTCCCAGCAG CCAAGCGGAA CTACATCCTG GGCCTGGACA
841 TCGGCATCAC CAGCGTGGGC TACGGCATCA TCGACTACGA GACACGGGAC GTGATCGATG
901 CCGGCGTGCG GCTGTTCAAA GAGGCCAACG TGGAAAACAA CGAGGGCAGG CGGAGCAAGA
961 GAGGCGCCAG AAGGCTGAAG CGGCGGAGGC GGCATAGAAT CCAGAGAGTG AAGAAGCTGC
1021 TGTTCGACTA CAACCTGCTG ACCGACCACA GCGAGCTGAG CGGCATCAAC CCCTACGAGG
1081 CCAGAGTGAA GGGCCTGAGC CAGAAGCTGA GCGAGGAAGA GTTCTCTGCC GCCCTGCTGC
1141 ACCTGGCCAA GAGAAGAGGC GTGCACAACG TGAACGAGGT GGAAGAGGAC ACCGGCAACG
1201 AGCTGTCCAC CAAAGAGCAG ATCAGCCGGA ACAGCAAGGC CCTGGAAGAG AAATACGTGG
1261 CCGAACTGCA GCTGGAACGG CTGAAGAAAG ACGGCGAAGT GCGGGGCAGC ATCAACAGAT
1321 TCAAGACCAG CGACTACGTG AAAGAAGCCA AACAGCTGCT GAAGGTGCAG AAGGCCTACC
1381 ACCAGCTGGA CCAGAGCTTC ATCGACACCT ACATCGACCT GCTGGAAACC CGGCGGACCT
1441 ACTATGAGGG ACCTGGCGAG GGCAGCCCCT TCGGCTGGAA GGACATCAAA GAATGGTACG
1501 AGATGCTGAT GGGCCACTGC ACCTACTTCC CCGAGGAACT GCGGAGCGTG AAGTACGCCT
1561 ACAACGCCGA CCTGTACAAC GCCCTGAACG ACCTGAACAA TCTCGTGATC ACCAGGGACG
1621 AGAACGAGAA GCTGGAATAT TACGAGAAGT TCCAGATCAT CGAGAACGTG TTCAAGCAGA
1681 AGAAGAAGCC CACCCTGAAG CAGATCGCCA AAGAAATCCT CGTGAACGAA GAGGATATTA
1741 AGGGCTACAG AGTGACCAGC ACCGGCAAGC CCGAGTTCAC CAACCTGAAG GTGTACCACG
1801 ACATCAAGGA CATTACCGCC CGGAAAGAGA TTATTGAGAA CGCCGAGCTG CTGGATCAGA
1861 TTGCCAAGAT CCTGACCATC TACCAGAGCA GCGAGGACAT CCAGGAAGAA CTGACCAATC
1921 TGAACTCCGA GCTGACCCAG GAAGAGATCG AGCAGATCTC TAATCTGAAG GGCTATACCG
1981 GCACCCACAA CCTGAGCCTG AAGGCCATCA ACCTGATCCT GGACGAGCTG TGGCACACCA
2041 ACGACAACCA GATCGCTATC TTCAACCGGC TGAAGCTGGT GCCCAAGAAG GTGGACCTGT
2101 CCCAGCAGAA AGAGATCCCC ACCACCCTGG TGGACGACTT CATCCTGAGC CCCGTCGTGA
2161 AGAGAAGCTT CATCCAGAGC ATCAAAGTGA TCAACGCCAT CATCAAGAAG TACGGCCTGC
2221 CCAACGACAT CATTATCGAG CTGGCCCGCG AGAAGAACTC CAAGGACGCC CAGAAAATGA
2281 TCAACGAGAT GCAGAAGCGG AACCGGCAGA CCAACGAGCG GATCGAGGAA ATCATCCGGA
2341 CCACCGGCAA AGAGAACGCC AAGTACCTGA TCGAGAAGAT CAAGCTGCAC GACATGCAGG
2401 AAGGCAAGTG CCTGTACAGC CTGGAAGCCA TCCCTCTGGA AGATCTGCTG AACAACCCCT
2461 TCAACTATGA GGTGGACCAC ATCATCCCCA GAAGCGTGTC CTTCGACAAC AGCTTCAACA
2521 ACAAGGTGCT CGTGAAGCAG GAAGAAAACA GCAAGAAGGG CAACCGGACC CCATTCCAGT
2581 ACCTGAGCAG CAGCGACAGC AAGATCAGCT ACGAAACCTT CAAGAAGCAC ATCCTGAATC
2641 TGGCCAAGGG CAAGGGCAGA ATCAGCAAGA CCAAGAAAGA GTATCTGCTG GAAGAACGGG
2701 ACATCAACAG GTTCTCCGTG CAGAAAGACT TCATCAACCG GAACCTGGTG GATACCAGAT
2761 ACGCCACCAG AGGCCTGATG AACCTGCTGC GGAGCTACTT CAGAGTGAAC AACCTGGACG
2821 TGAAAGTGAA GTCCATCAAT GGCGGCTTCA CCAGCTTTCT GCGGCGGAAG TGGAAGTTTA
2881 AGAAAGAGCG GAACAAGGGG TACAAGCACC ACGCCGAGGA CGCCCTGATC ATTGCCAACG
2941 CCGATTTCAT CTTCAAAGAG TGGAAGAAAC TGGACAAGGC CAAAAAAGTG ATGGAAAACC
3001 AGATGTTCGA GGAAAAGCAG GCCGAGAGCA TGCCCGAGAT CGAAACCGAG CAGGAGTACA
3061 AAGAGATCTT CATCACCCCC CACCAGATCA AGCACATTAA GGACTTCAAG GACTACAAGT
3121 ACAGCCACCG GGTGGACAAG AAGCCTAATA GAGAGCTGAT TAACGACACC CTGTACTCCA
3181 CCCGGAAGGA CGACAAGGGC AACACCCTGA TCGTGAACAA TCTGAACGGC CTGTACGACA
3241 AGGACAATGA CAAGCTGAAA AAGCTGATCA ACAAGAGCCC CGAAAAGCTG CTGATGTACC
3301 ACCACGACCC CCAGACCTAC CAGAAACTGA AGCTGATTAT GGAACAGTAC GGCGACGAGA
3361 AGAATCCCCT GTACAAGTAC TACGAGGAAA CCGGGAACTA CCTGACCAAG TACTCCAAAA
3421 AGGACAACGG CCCCGTGATC AAGAAGATTA AGTATTACGG CAACAAACTG AACGCCCATC
3481 TGGACATCAC CGACGACTAC CCCAACAGCA GAAACAAGGT CGTGAAGCTG TCCCTGAAGC
3541 CCTACAGATT CGACGTGTAC CTGGACAATG GCGTGTACAA GTTCGTGACC GTGAAGAATC
3601 TGGATGTGAT CAAAAAAGAA AACTACTACG AAGTGAATAG CAAGTGCTAT GAGGAAGCTA
3661 AGAAGCTGAA GAAGATCAGC AACCAGGCCG AGTTTATCGC CTCCTTCTAC AACAACGATC
3721 TGATCAAGAT CAACGGCGAG CTGTATAGAG TGATCGGCGT GAACAACGAC CTGCTGAACC
3781 GGATCGAAGT GAACATGATC GACATCACCT ACCGCGAGTA CCTGGAAAAC ATGAACGACA
3841 AGAGGCCCCC CAGGATCATT AAGACAATCG CCTCCAAGAC CCAGAGCATT AAGAAGTACA
3901 GCACAGACAT TCTGGGCAAC CTGTATGAAG TGAAATCTAA GAAGCACCCT CAGATCATCA
3961 AAAAGGGCAA AAGGCCGGCG GCCACGAAAA AGGCCGGCCA GGCAAAAAAG AAAAAGggat
4021 cctacccata cgatgttcca gattacgctt acccatacga tgttccagat tacgcttacc
4081 catacgatgt tccagattac gcttaagaat tcctagagct cgctgatcag cctcgactgt
4141 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
4201 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
4261 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
4321 agagaatagc aggcatgctg gggaggtacc tgagggccta tttcccatga ttccttcata
4381 tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac
4441 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
4501 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
4561 tttcttggct ttatatatct tGTGGAAAGG ACGAAACACC gGAGACCacg gcaGGTCTCa
4621 gttttagtac tctggaaaca gaatctacta aaacaaggca aaatgccgtg tttatctcgt
4681 caacttgttg gcgagatttt tgcggccgca ggaaccccta gtgatggagt tggccactcc
4741 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
4801 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
4861 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
4921 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
4981 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
5041 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
5101 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
5161 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
5221 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
5281 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
5341 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
5401 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
5461 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
5521 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
5581 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
5641 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
5701 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
5761 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
5821 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
5881 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
5941 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
6001 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
6061 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
6121 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
6181 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
6241 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
6301 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
6361 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
6421 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
6481 ggaagccgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
6541 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
6601 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
6661 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
6721 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
6781 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
6841 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
6901 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
6961 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
7021 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
7081 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
7141 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
7201 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
7261 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
7321 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
7381 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
7441 cacatgt
//
pX601 载体其他相关CRISPR/Cas9载体：
