pX600
pX600
编号 | 载体名称 |
北京华越洋VECT75267 | pX600 |
pX600 载体基本信息：
载体名称: | pX600 ; pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA |
质粒类型: | CRISPR-Cas9载体；基因敲除载体；哺乳动物载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶，多克隆位点 |
启动子: | -- |
载体大小: | 7101 bp |
5' 测序引物及序列: | CMV fwd:CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | BGH rev:TAGAAGGCACAGTCGAGG |
载体标签: | NLS (N ter)；NLS、3xHA(C ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | Stbl3 |
宿主细胞（系）: | 常规哺乳动物细胞等 |
备注: | A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA. |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pX600 载体质粒图谱和多克隆位点信息：
pX600 载体序列：
ORIGIN
1 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
61 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
481 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
661 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
721 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
781 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
841 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
901 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
961 ggcttccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1021 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1081 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1141 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1201 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1261 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1321 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1381 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1441 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1501 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1561 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1621 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1681 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1741 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1801 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
1861 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
1921 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
1981 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
2041 agcagattgt actgagagtg caccataaaa ttgtaaacgt taatattttg ttaaaattcg
2101 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
2161 cttataaatc aaaagaatag cccgagatag ggttgagtgt tgttccagtt tggaacaaga
2221 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
2281 atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg tgccgtaaag
2341 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
2401 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
2461 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
2521 cgtactatgg ttgctttgac gtatgcggtg tgaaataccg cacagatgcg taaggagaaa
2581 ataccgcatc aggcgcccct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg
2641 ggcaaagccc gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg
2701 cagagaggga gtggccaact ccatcactag gggttcctgc ggccgcctcg aggcgttgac
2761 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
2821 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
2881 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
2941 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
3001 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
3061 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
3121 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
3181 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
3241 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
3301 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actaccggtg
3361 ccaccATGGC CCCAAAGAAG AAGCGGAAGG TCGGTATCCA CGGAGTCCCA GCAGCCAAGC
3421 GGAACTACAT CCTGGGCCTG GACATCGGCA TCACCAGCGT GGGCTACGGC ATCATCGACT
3481 ACGAGACACG GGACGTGATC GATGCCGGCG TGCGGCTGTT CAAAGAGGCC AACGTGGAAA
3541 ACAACGAGGG CAGGCGGAGC AAGAGAGGCG CCAGAAGGCT GAAGCGGCGG AGGCGGCATA
3601 GAATCCAGAG AGTGAAGAAG CTGCTGTTCG ACTACAACCT GCTGACCGAC CACAGCGAGC
3661 TGAGCGGCAT CAACCCCTAC GAGGCCAGAG TGAAGGGCCT GAGCCAGAAG CTGAGCGAGG
3721 AAGAGTTCTC TGCCGCCCTG CTGCACCTGG CCAAGAGAAG AGGCGTGCAC AACGTGAACG
3781 AGGTGGAAGA GGACACCGGC AACGAGCTGT CCACCAAAGA GCAGATCAGC CGGAACAGCA
3841 AGGCCCTGGA AGAGAAATAC GTGGCCGAAC TGCAGCTGGA ACGGCTGAAG AAAGACGGCG
3901 AAGTGCGGGG CAGCATCAAC AGATTCAAGA CCAGCGACTA CGTGAAAGAA GCCAAACAGC
3961 TGCTGAAGGT GCAGAAGGCC TACCACCAGC TGGACCAGAG CTTCATCGAC ACCTACATCG
4021 ACCTGCTGGA AACCCGGCGG ACCTACTATG AGGGACCTGG CGAGGGCAGC CCCTTCGGCT
4081 GGAAGGACAT CAAAGAATGG TACGAGATGC TGATGGGCCA CTGCACCTAC TTCCCCGAGG
4141 AACTGCGGAG CGTGAAGTAC GCCTACAACG CCGACCTGTA CAACGCCCTG AACGACCTGA
4201 ACAATCTCGT GATCACCAGG GACGAGAACG AGAAGCTGGA ATATTACGAG AAGTTCCAGA
4261 TCATCGAGAA CGTGTTCAAG CAGAAGAAGA AGCCCACCCT GAAGCAGATC GCCAAAGAAA
4321 TCCTCGTGAA CGAAGAGGAT ATTAAGGGCT ACAGAGTGAC CAGCACCGGC AAGCCCGAGT
4381 TCACCAACCT GAAGGTGTAC CACGACATCA AGGACATTAC CGCCCGGAAA GAGATTATTG
4441 AGAACGCCGA GCTGCTGGAT CAGATTGCCA AGATCCTGAC CATCTACCAG AGCAGCGAGG
4501 ACATCCAGGA AGAACTGACC AATCTGAACT CCGAGCTGAC CCAGGAAGAG ATCGAGCAGA
4561 TCTCTAATCT GAAGGGCTAT ACCGGCACCC ACAACCTGAG CCTGAAGGCC ATCAACCTGA
4621 TCCTGGACGA GCTGTGGCAC ACCAACGACA ACCAGATCGC TATCTTCAAC CGGCTGAAGC
4681 TGGTGCCCAA GAAGGTGGAC CTGTCCCAGC AGAAAGAGAT CCCCACCACC CTGGTGGACG
4741 ACTTCATCCT GAGCCCCGTC GTGAAGAGAA GCTTCATCCA GAGCATCAAA GTGATCAACG
4801 CCATCATCAA GAAGTACGGC CTGCCCAACG ACATCATTAT CGAGCTGGCC CGCGAGAAGA
4861 ACTCCAAGGA CGCCCAGAAA ATGATCAACG AGATGCAGAA GCGGAACCGG CAGACCAACG
4921 AGCGGATCGA GGAAATCATC CGGACCACCG GCAAAGAGAA CGCCAAGTAC CTGATCGAGA
4981 AGATCAAGCT GCACGACATG CAGGAAGGCA AGTGCCTGTA CAGCCTGGAA GCCATCCCTC
5041 TGGAAGATCT GCTGAACAAC CCCTTCAACT ATGAGGTGGA CCACATCATC CCCAGAAGCG
5101 TGTCCTTCGA CAACAGCTTC AACAACAAGG TGCTCGTGAA GCAGGAAGAA AACAGCAAGA
5161 AGGGCAACCG GACCCCATTC CAGTACCTGA GCAGCAGCGA CAGCAAGATC AGCTACGAAA
5221 CCTTCAAGAA GCACATCCTG AATCTGGCCA AGGGCAAGGG CAGAATCAGC AAGACCAAGA
5281 AAGAGTATCT GCTGGAAGAA CGGGACATCA ACAGGTTCTC CGTGCAGAAA GACTTCATCA
5341 ACCGGAACCT GGTGGATACC AGATACGCCA CCAGAGGCCT GATGAACCTG CTGCGGAGCT
5401 ACTTCAGAGT GAACAACCTG GACGTGAAAG TGAAGTCCAT CAATGGCGGC TTCACCAGCT
5461 TTCTGCGGCG GAAGTGGAAG TTTAAGAAAG AGCGGAACAA GGGGTACAAG CACCACGCCG
5521 AGGACGCCCT GATCATTGCC AACGCCGATT TCATCTTCAA AGAGTGGAAG AAACTGGACA
5581 AGGCCAAAAA AGTGATGGAA AACCAGATGT TCGAGGAAAA GCAGGCCGAG AGCATGCCCG
5641 AGATCGAAAC CGAGCAGGAG TACAAAGAGA TCTTCATCAC CCCCCACCAG ATCAAGCACA
5701 TTAAGGACTT CAAGGACTAC AAGTACAGCC ACCGGGTGGA CAAGAAGCCT AATAGAGAGC
5761 TGATTAACGA CACCCTGTAC TCCACCCGGA AGGACGACAA GGGCAACACC CTGATCGTGA
5821 ACAATCTGAA CGGCCTGTAC GACAAGGACA ATGACAAGCT GAAAAAGCTG ATCAACAAGA
5881 GCCCCGAAAA GCTGCTGATG TACCACCACG ACCCCCAGAC CTACCAGAAA CTGAAGCTGA
5941 TTATGGAACA GTACGGCGAC GAGAAGAATC CCCTGTACAA GTACTACGAG GAAACCGGGA
6001 ACTACCTGAC CAAGTACTCC AAAAAGGACA ACGGCCCCGT GATCAAGAAG ATTAAGTATT
6061 ACGGCAACAA ACTGAACGCC CATCTGGACA TCACCGACGA CTACCCCAAC AGCAGAAACA
6121 AGGTCGTGAA GCTGTCCCTG AAGCCCTACA GATTCGACGT GTACCTGGAC AATGGCGTGT
6181 ACAAGTTCGT GACCGTGAAG AATCTGGATG TGATCAAAAA AGAAAACTAC TACGAAGTGA
6241 ATAGCAAGTG CTATGAGGAA GCTAAGAAGC TGAAGAAGAT CAGCAACCAG GCCGAGTTTA
6301 TCGCCTCCTT CTACAACAAC GATCTGATCA AGATCAACGG CGAGCTGTAT AGAGTGATCG
6361 GCGTGAACAA CGACCTGCTG AACCGGATCG AAGTGAACAT GATCGACATC ACCTACCGCG
6421 AGTACCTGGA AAACATGAAC GACAAGAGGC CCCCCAGGAT CATTAAGACA ATCGCCTCCA
6481 AGACCCAGAG CATTAAGAAG TACAGCACAG ACATTCTGGG CAACCTGTAT GAAGTGAAAT
6541 CTAAGAAGCA CCCTCAGATC ATCAAAAAGG GCAAAAGGCC GGCGGCCACG AAAAAGGCCG
6601 GCCAGGCAAA AAAGAAAAAG ggatccTACC CATACGATGT TCCAGATTAC GCTTACCCAT
6661 ACGATGTTCC AGATTACGCT TACCCATACG ATGTTCCAGA TTACGCTTAA GAAttcctag
6721 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
6781 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
6841 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
6901 ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggagC TAGAggccgc
6961 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
7021 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
7081 gagcgcgcag ctgcctgcag g
//
pX600 载体其他相关CRISPR/Cas9载体：
