PX395
PX395
编号 | 载体名称 |
北京华越洋VECT75228 | PX395 |
PX395 载体基本信息：
载体名称: | PX395 或 PX395 Roseburia intestinalis Cas9 |
质粒类型: | CRISPR-Cas9载体；基因敲除载体；昆虫细胞载体 |
高拷贝/低拷贝: | 高拷贝 |
插入位点: | 限制性内切酶，多克隆位点 |
启动子: | CMV |
载体大小: | 10616 bp |
5' 测序引物及序列: | CMV Forward:CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3') |
载体标签: | NLS (C ter)；HA、NLS (N ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | Neomycin |
克隆菌株: | Stbl3 |
宿主细胞（系）: | 昆虫细胞 |
备注: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 杆状病毒 |
PX395 载体质粒图谱和多克隆位点信息：
PX395 载体序列：
ORIGIN
1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
1861 aaaaagcgca aggtcgaagc gtccatgaga gagaacggga gtgacgaaag gcggaggaat
1921 atggacgaaa aaatggatta caggattggg ctggacatcg gcatcgcatc agtggggtgg
1981 gccgtcctgc agaacaattc agacgatgag cctgtgagga ttgtcgacct gggagtgcgc
2041 attttcgata ccgcagagat cccaaagaca ggcgaaagcc tggcaggacc ccggagagca
2101 gctcgaacca caaggcgccg actgcggaga aggaaacacc gcctggaccg aatcaagtgg
2161 ctgttcgaga accaggggct gatcaatatt gacgattttc tgaagagata caacatggcc
2221 ggactgccag atgtgtacca gctgcggtat gaggctctgg acagaaaact gaccgatgag
2281 gaactggccc aggtgctgct gcacatcgct aagcatcggg gcttcagaag caccaggaaa
2341 gccgagacag cagccaagga aaacggggca gtgctgaagg ccacagacga gaatcagaaa
2401 cgaatgcagg aaaagggata caggacagtg ggcgagatga tctacctgga cgaggccttc
2461 cggactggct gctcttggag tgagaaaggg tacatcctga ccccccgcaa caaggctgaa
2521 aattatcagc acacaatgct gcgggcaatg ctggtggagg aagtcaagga gattttcagc
2581 tcccagcgcc gactgggcaa cgaaaaagcc actgaggaac tggaggaaaa gtacctggag
2641 atcatgacct cccagcgctc ttttgacctg gggcctggaa tgcagccaga tgggaagccc
2701 tccccttatg caatggaggg cttctctgac agagtgggga aatgtacttt tctgggggat
2761 cagggagagc tgaggggcgc taaggggacc tacacagccg aatatttcgt ggctctgcag
2821 aaaatcaacc acacaaagct ggtcaatcag gacggcgaga caaggaattt cactgaggaa
2881 gagcggagag ccctgactct gctgctgttt acccagaaag aggtgaagta cgctgcagtc
2941 cgcaagaaac tgggcctgcc tgaggacatc ctgttctaca acctgaacta caagaaggcc
3001 gctactaaag aagagcagca gaaggagaac cagaataccg aaaaagccaa gtttatcggg
3061 atgccatact atcacgatta caagaaatgc ctggaagaga gagtgaagta tctgaccgag
3121 aacgaagtca gggacctgtt tgatgagatc ggaatgattc tgacttgtta caaaaatgac
3181 gattcccgca ccgaacgact ggccaagctg ggactggtgc ccatcgagat ggaaggcctg
3241 ctggcttata ctcctaccaa attccagcat ctgtctatga aggcaatgcg gaacatcatt
3301 ccctttctgg agaaagggat gacctacgac aaggcttgcg aagaggcagg atatgacttc
3361 aaagccgata gcaaggggac taaacagaag ctgctgaccg gagagaacgt gaatcagaca
3421 atcaacgaaa ttactaatcc tgtggtcaaa cgctcagtga gccagacagt gaaggtcatt
3481 aacgccatca ttcggactta cggcagtcca caggctatca atattgagct ggcaagagaa
3541 atgtcaaaga cctttgaaga gaggcgcaaa atcaaggggg acatggagaa acggcagaag
3601 aacaatgaag atgtgaagaa acagattcag gagctgggaa aactgtctcc tacaggccag
3661 gacatcctga agtacagact gtggcaggag cagcagggga tttgtatgta tagtggaaaa
3721 accatcccac tggaagagct gttcaagccc ggctacgaca tcgatcacat tctgccctat
3781 tcaattacat tcgacgatag ctttaggaac aaagtgctgg tcacatccca ggagaacaga
3841 cagaagggca ataggactcc ttacgagtat atggggaacg acgaacagcg ctggaatgag
3901 tttgaaacca gggtgaaaac taccatccgc gattacaaga aacagcagaa gctgctgaag
3961 aaacatttct ctgaagagga aaggagtgag tttaaagaac ggaacctgac agacactaag
4021 tacatcacaa ccgtgatcta caacatgatc agacagaatc tggagatggc ccccctgaac
4081 cgccctgaaa agaaaaagca ggtgcgggct gtcaatggcg caattaccgc ctacctgcga
4141 aaacggtggg ggctgccaca gaagaatcgg gagacagaca cacaccatgc tatggatgca
4201 gtggtcatcg cctgctgtac cgacggcatg atccagaaaa ttagtagata cacaaaggtg
4261 agagagaggt gctattcaaa gggaacagag ttcgtcgatg cagagactgg cgaaatcttt
4321 agacccgagg actacagcag ggccgagtgg gatgaaattt tcggcgtgca catcccaaag
4381 ccctgggaga catttcgcgc cgaactggac gtccgaatgg gggacgatcc aaagggattc
4441 ctggacactc atagcgatgt ggctctggag ctggattatc ccgagtacat ctacgaaaac
4501 ctgcggccta tcttcgtgag cagaatgcca aatcacaagg tcaccggagc agcccatgct
4561 gacacaattc ggtccccaag acactttaaa gatgagggca tcgtgctgac taagaccgca
4621 ctgaccgacc tgaaactgga caaggatggg gagatcgacg gatactataa cccccagtcc
4681 gatctgctgc tgtacgaagc actgaaaaag cagctgctgc tgtatggcaa tgatgccaaa
4741 aaggccttcg ctcaggactt tcataaaccc aaggccgatg gaactgaggg ccctgtggtc
4801 aggaaggtga agatccagaa aaagcagacc atgggagtgt tcgtcgactc tggcaacggg
4861 attgccgaga atggcgggat ggtgcgcatc gatgtgttcc gagtcaacgg caagtactat
4921 tttgtgcccg tctacaccgc tgacgtggtc aaaaaggtgc tgcctaatag ggccagtaca
4981 gctcacaagc catacggcga gtggaaagtg atggaggaca aggatttcct gtttagtctg
5041 tattcacgcg acctgatcca tatcaagtct aaaaaggata tccctattaa gatggtgaac
5101 ggaggcatgg aggggatcaa ggaaacctac gcatactata ttggagccga catcagcgct
5161 gcaaatatcc agggcattgc ccacgattcc aggtataaat tccgcggact gggcattcag
5221 tctctggacg tgctggagaa gtgtcagatc gatgtgctgg gacatgtcag cgtggtccga
5281 tccgaaaagc ggatgggctt tagctaagaa ttcggtaccg agctcaagct tgcggccgcc
5341 tatagtgtca cctaaatcgt aacccagctt tcttgtacaa agtggtcggg tggcatccct
5401 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
5461 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
5521 tggggtggag gggggtggta tggagcaagg ggcaagttgg gatgacaacc tgtagggcct
5581 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
5641 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
5701 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
5761 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
5821 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctagca taaccccttg
5881 gggcctctaa acgggtcttg aggggttttt tgtctagaag acaatagcag gcatgctggg
5941 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
6001 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
6061 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
6121 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
6181 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
6241 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
6301 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
6361 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
6421 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
6481 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
6541 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
6601 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
6661 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
6721 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
6781 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
6841 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
6901 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
6961 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
7021 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
7081 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
7141 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
7201 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
7261 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
7321 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
7381 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
7441 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
7501 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
7561 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
7621 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
7681 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
7741 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
7801 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
7861 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
7921 caatgtatct tatcatgtct gttcatgaag ccccttgagc atctgacttc tggctaataa
7981 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
8041 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
8101 acatatgccc atatgtaact agcataaccc cttggggcct ctaaacgggt cttgaggggt
8161 tttttgctga aagcatgcgg aggaaattct ccttgaagtt tccctggtgt tcaaagtaaa
8221 ggagtttgca ccagacgcac ctctgttcac tggtccggcg tattaaaaca cgatacattg
8281 ttattagtac atttattaag cgctagattc tgtgcgttgt tgatttacag acaattgttg
8341 tacgtatttt aataattcat taaatttata atctttaggg tggtatgtta gagcgaaaat
8401 caaatgattt tcagcgtctt tatatctgaa tttaaatatt aaatcctcaa tagatttgta
8461 aaataggttt cgattagttt caaacaaggg ttgtttttcc gaaccgatgg ctggactatc
8521 taatggattt tcgctcaacg ccacaaaact tgccaaatct tgtagcagca atctagcttt
8581 gtcgatattc gtttgtgttt tgttttgtaa taaaggttcg acgtcgttca aaatattatg
8641 cgcttttgta tttctttcat cactgtcgtt agtgtacaat tgactcgacg taaacacgtt
8701 aaatagagct tggacatatt taacatcggg cgtgttagct ttattaggcc gattatcgtc
8761 gtcgtcccaa ccctcgtcgt tagaagttgc ttccgaagac gattttgcca tagccacacg
8821 acgcctatta attgtgtcgg ctaacacgtc cgcgatcaaa tttgtagttg agctttttgg
8881 aattgcgatc gcataacttc gtatagcata cattatacga agttataagc tcggaacgct
8941 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
9001 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
9061 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc ccctgacgag
9121 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
9181 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
9241 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
9301 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
9361 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
9421 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
9481 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
9541 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
9601 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
9661 cgcagaaaaa aaggatctca agaagatcct ttgttaccaa tgcttaatca gtgaggcacc
9721 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
9781 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
9841 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
9901 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
9961 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
10021 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
10081 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
10141 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
10201 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
10261 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
10321 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
10381 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
10441 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
10501 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
10561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgtccg cgcgtt
//
PX395 载体其他相关CRISPR/Cas9载体：
