PX393
PX393
编号 | 载体名称 |
北京华越洋VECT75229 | PX393 |
PX393 载体基本信息：
载体名称: | PX393 或 PX393 Gluconacetobacter diazotrophicus Cas9 |
质粒类型: | CRISPR-Cas9载体；基因敲除载体；昆虫细胞载体 |
高拷贝/低拷贝: | 高拷贝 |
插入位点: | 限制性内切酶，多克隆位点 |
启动子: | CMV |
载体大小: | 10346 bp |
5' 测序引物及序列: | CMV Forward:CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3') |
载体标签: | NLS (C ter)；HA、NLS (N ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | Neomycin |
克隆菌株: | Stbl3 |
宿主细胞（系）: | 昆虫细胞 |
备注: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 杆状病毒 |
PX393 载体质粒图谱和多克隆位点信息：
PX393 载体序列：
ORIGIN
1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
1861 aaaaagcgca aggtcgaagc gtccatggga gagaatatga ttgacgagag tctgaccttc
1921 ggcattgacc tggggattgg gagttgtggg tgggcagtgc tgaggcggcc aagcgccttc
1981 ggacggaagg gcgtgatcga gggaatgggc tcctggtgct ttgacgtccc cgagacatct
2041 aaagaacgga ctcctaccaa ccagatccgg agatccaata gactgctgag gcgcgtgatc
2101 cgacggagaa ggaaccggat ggccgctatt cgccgactgc tgcacgcagc aggcctgctg
2161 ccatcaaccg acagcgatgc cctgaagcgg cccggacatg atccttggga actgagggca
2221 cgaggcctgg acaagccact gaaacccgtg gagttcgctg tggtcctggg ccacatcgca
2281 aagcggagag ggtttaaatc cgctgcaaag agaaaagcca caaacattag ctccgacgat
2341 aagaaaatgc tgacagccct ggaggctact cgagaacggc tggggagata caggaccgtg
2401 ggagaaatgt tcgccaggga ccctgatttt gcttctaggc gccgaaatcg cgagggcaag
2461 tatgatagga ccacagctcg cgacgatctg gagcacgaag tgcacgccct gttcgcagct
2521 cagcggagac tgggacaggg atttgccagt ccagaactgg aggaggcctt caccgcttca
2581 gcatttcatc agaggcccat gcaggacagc gagcgcctgg tgggattctg cccttttgag
2641 cgaaccgaaa agcgggcagc caaactgaca ccctctttcg agcgctttcg actgctggcc
2701 cggctgctga acctgagaat cactacccca gacggagagc ggcccctgac agtggatgaa
2761 attgctctgg tcacccggga cctgggcaag accgcaaaac tgagtatcaa gcgggtgaga
2821 actctgattg gactggagga caatcagagg ttcacaacta tccgccccga ggacgaagat
2881 cgagacattg tggctcggac aggcggggca atgacaggga ctgccaccct gaggaaggca
2941 ctgggagagg ccctgtggac tgatatgcag gagcgccctg aacagctgga cgctatcgtg
3001 caggtcctga gcttctttga ggccaacgaa acaatcactg agaagctgag ggaaattggc
3061 ctgactctgg ccgtgctgga cgtcctgctg accgcactgg atgccggagt gttcgccaag
3121 tttaaaggcg ctgcacacat cagcaccaaa gccgctagga atctgctgcc acatctggag
3181 cagggcaggc gctacgatga ggcctgcaca atggcagggt atgaccacgc agcctcccgc
3241 ctgtctcacc atggccagat cgtggcaaag acacagttca acgccctggt cactgagatc
3301 ggcgaatcca ttgccaatcc aatcgctcgg aaggcactga tcgaggggct gaaacagatt
3361 tgggccatga gaaaccactg ggggctgccc ggaagtatcc atgtggagct ggcccgggat
3421 gtcggcaact caattgaaaa gcgacgggag attgaaaagc acatcgagaa aaatactgcc
3481 ctgagggctc gcgagagaag ggaggtgcat gatctgctgg acctggaaga tgtcaatggc
3541 gacaccctgc tgcgataccg gctgtggaag gagcagggag gcaaatgcct gtatacaggg
3601 aaggccatcc acattcggca gatcgctgca actgacaact ccgtgcaggt cgatcatatt
3661 ctgccttgga gccggttcgg cgacgatagt tttaacaaca agaccctgtg tctggcctct
3721 gctaatcagc agaagaaaag gtcaacacca tacgagtggc tgagcggcca gactggggat
3781 gcatggaacg ccttcgtgca gcgcatcgag acaaataagg aactgagagg gtttaagaaa
3841 aggaactatc tgctgaagaa tgctaaagag gcagaggaaa aattcagaag caggaacctg
3901 aatgacacca gatacgccgc taggctgttc gcagaggccg tgaagctgct gtatgccttt
3961 ggggagagac aggaaaaagg gggaaaccgc cgagtgttta ctcggcctgg agcactgacc
4021 gcagcactga gacaggcttg gggagtggag agcctgaaga aacaggatgg gaagcgcatc
4081 aatgacgatc gacaccatgc cctggatgct ctgaccgtgg ctgcagtcga cgaggccgaa
4141 attcagaggc tgacaaaatc attccacgag tgggaacagc agggcctggg gcggcctctg
4201 cggagagtgg agccaccttg ggagagcttc cgggcagacg tcgaggctac ctaccctgaa
4261 gtgtttgtcg cacggccaga gaggcgccga gcaagaggag aaggccatgc cgctaccatc
4321 cggcaggtga aggagagaga atgcacacca attgtgtttg agagaaaggc tgtctctagt
4381 ctgaaagagg cagacctgga acgaatcaaa gatggcgagc gcaacgaagc aattgtggag
4441 gccatcagga gctggattgc cactggacgc ccagctgatg caccaccacg ctccccccga
4501 ggcgacatca ttaccaagat caggctggcc accaccatca aggcagccgt gcctgtccgc
4561 ggagggaccg caggaagggg agaaatggtg cgcgcagatg tgttcagcaa gccaaaccgg
4621 agagggaaag acgagtggta tctggtgccc gtgtatccac accagatcat gaacaggaag
4681 gcttggccca aacctccaat gcgctcaatt gtggccaata aggatgagga cgaatggacc
4741 gaagtgggac ctgaacacca gttccggttt agcctgtacc ctagatccaa tatcgagatc
4801 attaggccat ctggagaagt gatcgaagga tatttcgtcg gcctgcatcg caacactggc
4861 gctctgacca tcagtgcaca caatgatccc aagagtatcc attcaggcat tgggaccaag
4921 acactgctgg ccatttccaa ataccaggtg gacagattcg gcagaaagtc tccagtgcgc
4981 aaagaggtcc gaacttggca cggggaagcc tgtatctctc ccaccccccc tggataagaa
5041 ttcggtaccg agctcaagct tgcggccgcc tatagtgtca cctaaatcgt aacccagctt
5101 tcttgtacaa agtggtcggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
5161 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
5221 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
5281 ggcaagttgg gatgacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
5341 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
5401 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
5461 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
5521 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
5581 ccttccctgt ccttctagca taaccccttg gggcctctaa acgggtcttg aggggttttt
5641 tgtctagaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
5701 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
5761 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
5821 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
5881 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
5941 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
6001 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
6061 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
6121 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
6181 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
6241 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
6301 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
6361 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
6421 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
6481 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
6541 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
6601 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
6661 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
6721 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
6781 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
6841 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
6901 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
6961 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
7021 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
7081 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
7141 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
7201 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
7261 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
7321 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
7381 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
7441 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
7501 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
7561 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
7621 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gttcatgaag
7681 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
7741 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
7801 cagaatgagt atttggttta gagtttggca acatatgccc atatgtaact agcataaccc
7861 cttggggcct ctaaacgggt cttgaggggt tttttgctga aagcatgcgg aggaaattct
7921 ccttgaagtt tccctggtgt tcaaagtaaa ggagtttgca ccagacgcac ctctgttcac
7981 tggtccggcg tattaaaaca cgatacattg ttattagtac atttattaag cgctagattc
8041 tgtgcgttgt tgatttacag acaattgttg tacgtatttt aataattcat taaatttata
8101 atctttaggg tggtatgtta gagcgaaaat caaatgattt tcagcgtctt tatatctgaa
8161 tttaaatatt aaatcctcaa tagatttgta aaataggttt cgattagttt caaacaaggg
8221 ttgtttttcc gaaccgatgg ctggactatc taatggattt tcgctcaacg ccacaaaact
8281 tgccaaatct tgtagcagca atctagcttt gtcgatattc gtttgtgttt tgttttgtaa
8341 taaaggttcg acgtcgttca aaatattatg cgcttttgta tttctttcat cactgtcgtt
8401 agtgtacaat tgactcgacg taaacacgtt aaatagagct tggacatatt taacatcggg
8461 cgtgttagct ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt tagaagttgc
8521 ttccgaagac gattttgcca tagccacacg acgcctatta attgtgtcgg ctaacacgtc
8581 cgcgatcaaa tttgtagttg agctttttgg aattgcgatc gcataacttc gtatagcata
8641 cattatacga agttataagc tcggaacgct gcgctcggtc gttcggctgc ggcgagcggt
8701 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
8761 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
8821 gtttttccat aggctccgcc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
8881 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
8941 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
9001 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
9061 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
9121 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
9181 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
9241 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
9301 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
9361 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
9421 ttgttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
9481 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
9541 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
9601 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
9661 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
9721 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
9781 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
9841 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
9901 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
9961 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
10021 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
10081 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
10141 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
10201 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
10261 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
10321 gggttattgt ctcatgtccg cgcgtt
//
PX393 载体其他相关CRISPR/Cas9载体：
