北京华越洋生物
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首页    载体及质粒    pBABE-Puro
pBABE-puroMap

pBABE-Puro

pBABE-Puro

pBABE-Puro

 



编号

载体名称

北京华越洋VECT55125

pBABE-Puro

 

pBABE-Puro载体基本信息:

载体名称:

pBABE-puro

质粒类型:

逆病毒载体

高拷贝/低拷贝:

高拷贝

克隆方法:

限制性内切酶,多克隆位点

启动子:

MSCV

载体大小:

5177 bp

5' 测序引物及序列:

pBABE 5' primer 5'CTTTATCCAGCCCTCAC3'

3' 测序引物及序列:

pBABE 3' primer   5'ACCCTAACTGACACACATTCC3'

载体标签:

载体抗性:

氨苄青霉素

筛选标记:

Puromycin

克隆菌株:

DH5αHB101

宿主细胞(系):

常规哺乳动物细胞系

备注:


 
推荐使用逆病毒包装载体:pUMVCpCMV-VSV-G
   

稳定性:

稳表达

组成型/诱导型:

组成型

病毒/非病毒:

逆转录病毒

 

pBABE-Puro载体质粒图谱和多克隆位点信息:

 

 

pBABE-Puro载体序列:

ORIGIN

    1 CTGCAGCCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA

   61 GGGCCAAGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT

  121 TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGTCCCCAGA TGCGGTCCAG CCCTCAGCAG

  181 TTTCTAGAGA ACCATCAGAT GTTTCCAGGG TGCCCCAAGG ACCTGAAATG ACCCTGTGCC

  241 TTATTTGAAC TAACCAATCA GTTCGCTTCT CGCTTCTGTT CGCGCGCTTC TGCTCCCCGA

  301 GCTCAATAAA AGAGCCCACA ACCCCTCACT CGGGGCGCCA GTCCTCCGAT TGACTGAGTC

  361 GCCCGGGTAC CCGTGTATCC AATAAACCCT CTTGCAGTTG CATCCGACTT GTGGTCTCGC

  421 TGTTCCTTGG GAGGGTCTCC TCTGAGTGAT TGACTACCCG TCAGCGGGGG TCTTTCATTT

  481 GGGGGCTCGT CCGGGATCGG GAGACCCCTG CCCAGGGACC ACCGACCCAC CACCGGGAGG

  541 TAAGCTGGCC AGCAACTTAT CTGTGTCTGT CCGATTGTCT AGTGTCTATG ACTGATTTTA

  601 TGCGCCTGCG TCGGTACTAG TTAGCTAACT AGCTCTGTAT CTGGCGGACC CGTGGTGGAA

  661 CTGACGAGTT CTGAACACCC GGCCGCAACC CTGGGAGACG TCCCAGGGAC TTTGGGGGCC

  721 GTTTTTGTGG CCCGACCTGA GGAAGGGAGT CGATGTGGAA TCCGACCCCG TCAGGATATG

  781 TGGTTCTGGT AGGAGACGAG AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT

  841 CGGTTTGGAA CCGAAGCCGC GCGTCTTGTC TGCTGCAGCA TCGTTCTGTG TTGTCTCTGT

  901 CTGACTGTGT TTCTGTATTT GTCTGAAAAT TAGGGCCAGA CTGTTACCAC TCCCTTAAGT

  961 TTGACCTTAG ATCACTGGAA AGATGTCGAG CGGCTCGCTC ACAACCAGTC GGTAGATGTC

 1021 AAGAAGAGAC GTTGGGTTAC CTTCTGCTCT GCAGAATGGC CAACCTTTAA CGTCGGATGG

 1081 CCGCGAGACG GCACCTTTAA CCGAGACCTC ATCACCCAGG TTAAGATCAA GGTCTTTTCA

 1141 CCTGGCCCGC ATGGACACCC AGACCAGGTC CCCTACATCG TGACCTGGGA AGCCTTGGCT

 1201 TTTGACCCCC CTCCCTGGGT CAAGCCCTTT GTACACCCTA AGCCTCCGCC TCCTCTTCTT

 1261 CCATCCGCGC CGTCTCTCCC CCTTGAACCT CCTCTTTCGA CCCCGCCTCA ATCCTCCCTT

 1321 TATCCAGCCC TCACTCCTTC TCTAGGCGCC GGCCGGATCC CAGTGTGGTG GTACGTAGGA

 1381 ATTCGCCAGC ACAGTGGTCG ACCCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC

 1441 CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT

 1501 GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT

 1561 CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG

 1621 CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT

 1681 CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA

 1741 AAAAGCTTAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC

 1801 CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG

 1861 TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG

 1921 TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA

 1981 CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG

 2041 AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC

 2101 GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA

 2161 AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC

 2221 CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA

 2281 CCGTCACCGC CGACGTCGAG TGCCCGAAGG ACCGCGCGAC CTGGTGCATG ACCCGCAAGC

 2341 CCGGTGCCTG ACGCCCGCCC CACGACCCGC AGCGCCCGAC CGAAAGGAGC GCACGACCCC

 2401 ATGCATCGAT AAAATAAAAG ATTTTATTTA GTCTCCAGAA AAAGGGGGGA ATGAAAGACC

 2461 CCACCTGTAG GTTTGGCAAG CTAGCTTAAG TAACGCCATT TTGCAAGGCA TGGAAAAATA

 2521 CATAACTGAG AATAGAGAAG TTCAGATCAA GGTCAGGAAC AGATGGAACA GCTGAATATG

 2581 GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA AGAACAGATG

 2641 GAACAGCTGA ATATGGGCCA AACAGGATAT CTGTGGTAAG CAGTTCCTGC CCCGGCTCAG

 2701 GGCCAAGAAC AGATGGTCCC CAGATGCGGT CCAGCCCTCA GCAGTTTCTA GAGAACCATC

 2761 AGATGTTTCC AGGGTGCCCC AAGGACCTGA AATGACCCTG TGCCTTATTT GAACTAACCA

 2821 ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC CCGAGCTCAA TAAAAGAGCC

 2881 CACAACCCCT CACTCGGGGC GCCAGTCCTC CGATTGACTG AGTCGCCCGG GTACCCGTGT

 2941 ATCCAATAAA CCCTCTTGCA GTTGCATCCG ACTTGTGGTC TCGCTGTTCC TTGGGAGGGT

 3001 CTCCTCTGAG TGATTGACTA CCCGTCAGCG GGGGTCTTTC ATTTCCGACT TGTGGTCTCG

 3061 CTGCCTTGGG AGGGTCTCCT CTGAGTGATT GACTACCCGT CAGCGGGGGT CTTCACATGC

 3121 AGCATGTATC AAAATTAATT TGGTTTTTTT TCTTAAGTAT TTACATTAAA TGGCCATAGT

 3181 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGC GCTCTTCCGC

 3241 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA

 3301 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG

 3361 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA

 3421 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA

 3481 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC

 3541 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC

 3601 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT

 3661 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG

 3721 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG

 3781 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA

 3841 CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG

 3901 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT

 3961 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT

 4021 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG

 4081 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TGCGGCCGCA

 4141 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG

 4201 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG

 4261 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC

 4321 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG

 4381 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG

 4441 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG

 4501 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT

 4561 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC

 4621 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC

 4681 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA

 4741 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAACAC

 4801 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT

 4861 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC

 4921 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA

 4981 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA

 5041 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT

 5101 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA

 5161 AAGTGCCAC

//

 

 

pBABE-Puro其他相关逆病毒载体:

pVSV-G

pBABE

pBABE-hygro-hTERT

pBABE-neo-SV40LT

pMKO.1-GFP

pBABE-neo

pBABE-GFP

pVPack-10A1

pBABE-Puro

pCMV-Gag-Pol

pVPack-Eco

pCMV-VSV-G

pCL-Eco

pCMV-VSV-G

pMSCV-PIG

pFB-ERV

pFB-hrGFP

pFB-Neo

pCFB

pRetroX-Tight-Pur-Luc

pRetroX-TetOne

pRetroX-PTuner2

pRetroX-PTuner-IRES

pRetroX-TRE3G-Luc

pRetroX-Tet-On     Advanced

pRetroQ-mCherry-N1

pRetroX-Tight-Pur

pRetroQ-AcGFP1-C1

pRetroQ-DsRed-Monomer-C1

RNAi-Ready     pSIREN-RetroQ-DsRed-Express

pRetroX-SG2M-Cyan

pQCXIX

pLXSN

RNAi-Ready     pSIREN-RetroQ-ZsGreen1

PSUPER.RETRO.PURO

pMSCVhyg

PRETROX-IRES-ZSGREEN1

pSuper.Retro.Neo

pMSCVpuro

pMKO.1-puro

pMSCV-FLIP-puro-dsRed-GFP-miRNA

pBABE-HAII

pBABE-puro-IRES-EGFP

pBABE-Puro     SV40LT

pBABE-neo-hTERT

pBABE-zeo

pBABE-hygro

pVPack-VSV-G

pVPack-GP

pCL-Ampho

pVPack-Ampho

pCMV-VSVG

pUMVC

pCFB-EGSH

pQCXIN

pFB

pFB-Luc

pRetroX-TetOne-luc

pRetro-Lib

pRetroX-PTuner2-C

pRetroX-PTuner

pRetroX-Tet3G

pRetroX-TRE3G

pRetroX-Tight-Hyg-Luc

pRetroX-Tet-Off     Advanced

pRetroQ-mCherry-C1

pRetroX-G1-Red

pRetroQ-AcGFP1-N1

pRetroQ-DsRed-Monomer-N1

pLXIN

pLNCX2

pSuper.Retro.Neo+GFP

pSilencer5.1-U6     Retro

pRetroX-IRES-DsRedExpress

RNAi-Ready     pSIREN-RetroQ

pMSCVneo

pQCXIH

pSilencer5.1-H1     Retro

pCLXSN