pBABE-Puro SV40LT
pBABE-Puro SV40LT
编号 | 载体名称 |
北京华越洋VECT55183 | pBABE-Puro SV40LT |
pBABE-Puro SV40LT逆病毒载体基本信息：
载体名称: | pBABE-Puro SV40LT, pBABEpuro SV40LT, pBABE Puro SV40 Large T |
质粒类型: | 哺乳动物细胞逆病毒基因表达载体 |
插入基因: | SV40 LT, SV40gp6 |
插入基因Genbank ID: | AF316141 |
插入位点: | BamH I (5’端）/ BamH I (3'端) |
高拷贝/低拷贝: | 低拷贝 |
启动子: | PGK |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 7302 bp |
5' 测序引物及序列: | pBABE 5‘: CTTTATCCAGCCCTCAC |
3' 测序引物及序列: | pBABE 3’: ACCCTAACTGACACACATTCC |
载体标签: | -- |
载体抗性: | 氨苄 |
筛选标记: | 嘌呤霉素(Puromycin) |
备注: | 含有嘌呤霉素筛选基因，可以用来筛选感染稳定的细胞系。 |
稳定性: | 稳表达 |
组成型: | 组成型 |
病毒/非病毒: | 逆病毒 |
pBABE-Puro SV40LT载体质粒图谱和多克隆位点信息：
pBABE-Puro SV40LT载体序列：
ORIGIN
1 CTGCAGCCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA
61 GGGCCAAGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT
121 TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGTCCCCAGA TGCGGTCCAG CCCTCAGCAG
181 TTTCTAGAGA ACCATCAGAT GTTTCCAGGG TGCCCCAAGG ACCTGAAATG ACCCTGTGCC
241 TTATTTGAAC TAACCAATCA GTTCGCTTCT CGCTTCTGTT CGCGCGCTTC TGCTCCCCGA
301 GCTCAATAAA AGAGCCCACA ACCCCTCACT CGGGGCGCCA GTCCTCCGAT TGACTGAGTC
361 GCCCGGGTAC CCGTGTATCC AATAAACCCT CTTGCAGTTG CATCCGACTT GTGGTCTCGC
421 TGTTCCTTGG GAGGGTCTCC TCTGAGTGAT TGACTACCCG TCAGCGGGGG TCTTTCATTT
481 GGGGGCTCGT CCGGGATCGG GAGACCCCTG CCCAGGGACC ACCGACCCAC CACCGGGAGG
541 TAAGCTGGCC AGCAACTTAT CTGTGTCTGT CCGATTGTCT AGTGTCTATG ACTGATTTTA
601 TGCGCCTGCG TCGGTACTAG TTAGCTAACT AGCTCTGTAT CTGGCGGACC CGTGGTGGAA
661 CTGACGAGTT CTGAACACCC GGCCGCAACC CTGGGAGACG TCCCAGGGAC TTTGGGGGCC
721 GTTTTTGTGG CCCGACCTGA GGAAGGGAGT CGATGTGGAA TCCGACCCCG TCAGGATATG
781 TGGTTCTGGT AGGAGACGAG AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT
841 CGGTTTGGAA CCGAAGCCGC GCGTCTTGTC TGCTGCAGCA TCGTTCTGTG TTGTCTCTGT
901 CTGACTGTGT TTCTGTATTT GTCTGAAAAT TAGGGCCAGA CTGTTACCAC TCCCTTAAGT
961 TTGACCTTAG ATCACTGGAA AGATGTCGAG CGGCTCGCTC ACAACCAGTC GGTAGATGTC
1021 AAGAAGAGAC GTTGGGTTAC CTTCTGCTCT GCAGAATGGC CAACCTTTAA CGTCGGATGG
1081 CCGCGAGACG GCACCTTTAA CCGAGACCTC ATCACCCAGG TTAAGATCAA GGTCTTTTCA
1141 CCTGGCCCGC ATGGACACCC AGACCAGGTC CCCTACATCG TGACCTGGGA AGCCTTGGCT
1201 TTTGACCCCC CTCCCTGGGT CAAGCCCTTT GTACACCCTA AGCCTCCGCC TCCTCTTCTT
1261 CCATCCGCGC CGTCTCTCCC CCTTGAACCT CCTCTTTCGA CCCCGCCTCA ATCCTCCCTT
1321 TATCCAGCCC TCACTCCTTC TCTAGGCGCC GGCCGGATCC ATGGATAAAG TTTTAAACAG
1381 AGAGGAATCT TTGCAGCTAA TGGACCTTCT AGGTCTTGAA AGGAGTGCCT GGGGGAATAT
1441 TCCTCTGATG AGAAAGGCAT ATTTAAAAAA ATGCAAGGAG TTTCATCCTG ATAAAGGAGG
1501 AGATGAAGAA AAAATGAAGA AAATGAATAC TCTGTACAAG AAAATGGAAG ATGGAGTAAA
1561 ATATGCTCAT CAACCTGACT TTGGAGGCTT CTGGGATGCA ACTGAGATTC CAACCTATGG
1621 AACTGATGAA TGGGAGCAGT GGTGGAATGC CTTTAATGAG GAAAACCTGT TTTGCTCAGA
1681 AGAAATGCCA TCTAGTGATG ATGAGGCTAC TGCTGACTCT CAACATTCTA CTCCTCCAAA
1741 AAAGAAGAGA AAGGTAGAAG ACCCCAAGGA CTTTCCTTCA GAATTGCTAA GTTTTTTGAG
1801 TCATGCTGTG TTTAGTAATA GAACTCTTGC TTGCTTTGCT ATTTACACCA CAAAGGAAAA
1861 AGCTGCACTG CTATACAAGA AAATTATGGA AAAATATTCT GTAACCTTTA TAAGTAGGCA
1921 TAACAGTTAT AATCATAACA TACTGTTTTT TCTTACTCCA CACAGGCATA GAGTGTCTGC
1981 TATTAATAAC TATGCTCAAA AATTGTGTAC CTTTAGCTTT TTAATTTGTA AAGGGGTTAA
2041 TAAGGAATAT TTGATGTATA GTGCCTTGAC TAGAGATCCA TTTTCTGTTA TTGAGGAAAG
2101 TTTGCCAGGT GGGTTAAAGG AGCATGATTT TAATCCAGAA GAAGCAGAGG AAACTAAACA
2161 AGTGTCCTGG AAGCTTGTAA CAGAGTATGC AATGGAAACA AAATGTGATG ATGTGTTGTT
2221 ATTGCTTGGG ATGTACTTGG AATTTCAGTA CAGTTTTGAA ATGTGTTTAA AATGTATTAA
2281 AAAAGAACAG CCCAGCCACT ATAAGTACCA TGAAAAGCAT TATGCAAATG CTGCTATATT
2341 TGCTGACAGC AAAAACCAAA AAACCATATG CCAACAGGCT GTTGATACTG TTTTAGCTAA
2401 AAAGCGGGTT GATAGCCTAC AATTAACTAG AGAACAAATG TTAACAAACA GATTTAATGA
2461 TCTTTTGGAT AGGATGGATA TAATGTTTGG TTCTACAGGC TCTGCTGACA TAGAAGAATG
2521 GATGGCTGGA GTTGCTTGGC TACACTGTTT GTTGCCCAAA ATGGATTCAG TGGTGTATGA
2581 CTTTTTAAAA TGCATGGTGT ACAACATTCC TAAAAAAAGA TACTGGCTGT TTAAAGGACC
2641 AATTGATAGT GGTAAAACTA CATTAGCAGC TGCTTTGCTT GAATTATGTG GGGGGAAAGC
2701 TTTAAATGTT AATTTGCCCT TGGACAGGCT GAACTTTGAG CTAGGAGTAG CTATTGACCA
2761 GTTTTTAGTA GTTTTTGAGG ATGTAAAGGG CACTGGAGGG GAGTCCAGAG ATTTGCCTTC
2821 AGGTCAGGGA ATTAATAACC TGGACAATTT AAGGGATTAT TTGGATGGCA GTGTTAAGGT
2881 AAACTTAGAA AAGAAACACC TAAATAAAAG AACTCAAATA TTTCCCCCTG GAATAGTCAC
2941 CATGAATGAG TACAGTGTGC CTAAAACACT GCAGGCCAGA TTTGTAAAAC AAATAGATTT
3001 TAGGCCCAAA GATTATTTAA AGCATTGCCT GGAACGCAGT GAGTTTTTGT TAGAAAAGAG
3061 AATAATTCAA AGTGGCATTG CTTTGCTTCT TATGTTAATT TGGTACAGAC CTGTGGCTGA
3121 GTTTGCTCAA AGTATTCAGA GCAGAATTGT GGAGTGGAAA GAGAGATTGG ACAAAGAGTT
3181 TAGTTTGTCA GTGTATCAAA AAATGAAGTT TAATGTGGCT ATGGGAATTG GAGTTTTAGA
3241 TTGGCTAAGA AACAGTGATG ATGATGATGA AGACAGCCAG GAAAATGCTG ATAAAAATGA
3301 AGATGGTGGG GAGAAGAACA TGGAAGACTC AGGGCATGAA ACAGGCATTG ATTCACAGTC
3361 CCAAGGCTCA TTTCAGGCCC CTCAGTCCTC ACAGTCTGTT CATGATCATA ATCAGCCATA
3421 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA
3481 AACATAAGGA TCCCAGTGTG GTGGTACGTA GGAATTCGCC AGCACAGTGG TCGACCCTGT
3541 GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC
3601 AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG
3661 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC
3721 CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA
3781 TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT
3841 GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT TACCATGACC GAGTACAAGC
3901 CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG
3961 CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC ATCGAGCGGG
4021 TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG
4081 TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG
4141 CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC
4201 AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG
4261 CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC
4321 CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC
4381 GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGTGCCCGA
4441 AGGACCGCGC GACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGACGCCCG CCCCACGACC
4501 CGCAGCGCCC GACCGAAAGG AGCGCACGAC CCCATGCATC GATAAAATAA AAGATTTTAT
4561 TTAGTCTCCA GAAAAAGGGG GGAATGAAAG ACCCCACCTG TAGGTTTGGC AAGCTAGCTT
4621 AAGTAACGCC ATTTTGCAAG GCATGGAAAA ATACATAACT GAGAATAGAG AAGTTCAGAT
4681 CAAGGTCAGG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA
4741 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGAACAGC TGAATATGGG CCAAACAGGA
4801 TATCTGTGGT AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG AACAGATGGT CCCCAGATGC
4861 GGTCCAGCCC TCAGCAGTTT CTAGAGAACC ATCAGATGTT TCCAGGGTGC CCCAAGGACC
4921 TGAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC TTCTGTTCGC
4981 GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC CCTCACTCGG GGCGCCAGTC
5041 CTCCGATTGA CTGAGTCGCC CGGGTACCCG TGTATCCAAT AAACCCTCTT GCAGTTGCAT
5101 CCGACTTGTG GTCTCGCTGT TCCTTGGGAG GGTCTCCTCT GAGTGATTGA CTACCCGTCA
5161 GCGGGGGTCT TTCATTTCCG ACTTGTGGTC TCGCTGCCTT GGGAGGGTCT CCTCTGAGTG
5221 ATTGACTACC CGTCAGCGGG GGTCTTCACA TGCAGCATGT ATCAAAATTA ATTTGGTTTT
5281 TTTTCTTAAG TATTTACATT AAATGGCCAT AGTTGCATTA ATGAATCGGC CAACGCGCGG
5341 GGAGAGGCGG TTTGCGTATT GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC
5401 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC
5461 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
5521 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
5581 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
5641 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
5701 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
5761 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
5821 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
5881 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
5941 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG
6001 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
6061 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
6121 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
6181 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
6241 CCTTTTAAAT TAAAAATGAA GTTTGCGGCC GCAAATCAAT CTAAAGTATA TATGAGTAAA
6301 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT
6361 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT
6421 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT
6481 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT
6541 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA
6601 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG
6661 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT
6721 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG
6781 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG
6841 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC
6901 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA CACGGGATAA TACCGCGCCA CATAGCAGAA
6961 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC
7021 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT
7081 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG
7141 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA
7201 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA
7261 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC AC //
pBABE-Puro SV40LT其他相关逆病毒载体：
