北京华越洋生物
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首页    载体及质粒    pBABE-zeo
pBABE-zeoMap

pBABE-zeo

pBABE-zeo

pBABE-zeo

 



编号

载体名称

北京华越洋VECT55184

pBABE-zeo

 

pBABE-zeo逆病毒载体基本信息:

载体名称:

pBABE-zeo

质粒类型:

逆病毒载体

高拷贝/低拷贝:

高拷贝

克隆方法:

限制性内切酶,多克隆位点

启动子:

MSCV

载体大小:

4888bp

5' 测序引物及序列:

pBABE 5' primer 5'CTTTATCCAGCCCTCAC3'

3' 测序引物及序列:

pBABE 3' primer   5'ACCCTAACTGACACACATTCC3'

载体标签:

载体抗性:

氨苄青霉素

筛选标记:

Zeocin

克隆菌株:

DH5αHB101

宿主细胞(系):

常规哺乳动物细胞系

备注:


 
推荐使用逆病毒包装载体:pUMVCpCMV-VSV-G
   

稳定性:

稳表达

组成型/诱导型:

组成型

病毒/非病毒:

逆转录病毒

 

pBABE-zeo载体质粒图谱和多克隆位点信息:

 

 

载体简介

pBABE-zeo载体序列:

ORIGIN

    1 CTGCAGCCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA

   61 GGGCCAAGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT

  121 TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGTCCCCAGA TGCGGTCCAG CCCTCAGCAG

  181 TTTCTAGAGA ACCATCAGAT GTTTCCAGGG TGCCCCAAGG ACCTGAAATG ACCCTGTGCC

  241 TTATTTGAAC TAACCAATCA GTTCGCTTCT CGCTTCTGTT CGCGCGCTTC TGCTCCCCGA

  301 GCTCAATAAA AGAGCCCACA ACCCCTCACT CGGGGCGCCA GTCCTCCGAT TGACTGAGTC

  361 GCCCGGGTAC CCGTGTATCC AATAAACCCT CTTGCAGTTG CATCCGACTT GTGGTCTCGC

  421 TGTTCCTTGG GAGGGTCTCC TCTGAGTGAT TGACTACCCG TCAGCGGGGG TCTTTCATTT

  481 GGGGGCTCGT CCGGGATCGG GAGACCCCTG CCCAGGGACC ACCGACCCAC CACCGGGAGG

  541 TAAGCTGGCC AGCAACTTAT CTGTGTCTGT CCGATTGTCT AGTGTCTATG ACTGATTTTA

  601 TGCGCCTGCG TCGGTACTAG TTAGCTAACT AGCTCTGTAT CTGGCGGACC CGTGGTGGAA

  661 CTGACGAGTT CTGAACACCC GGCCGCAACC CTGGGAGACG TCCCAGGGAC TTTGGGGGCC

  721 GTTTTTGTGG CCCGACCTGA GGAAGGGAGT CGATGTGGAA TCCGACCCCG TCAGGATATG

  781 TGGTTCTGGT AGGAGACGAG AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT

  841 CGGTTTGGAA CCGAAGCCGC GCGTCTTGTC TGCTGCAGCA TCGTTCTGTG TTGTCTCTGT

  901 CTGACTGTGT TTCTGTATTT GTCTGAAAAT TAGGGCCAGA CTGTTACCAC TCCCTTAAGT

  961 TTGACCTTAG ATCACTGGAA AGATGTCGAG CGGCTCGCTC ACAACCAGTC GGTAGATGTC

 1021 AAGAAGAGAC GTTGGGTTAC CTTCTGCTCT GCAGAATGGC CAACCTTTAA CGTCGGATGG

 1081 CCGCGAGACG GCACCTTTAA CCGAGACCTC ATCACCCAGG TTAAGATCAA GGTCTTTTCA

 1141 CCTGGCCCGC ATGGACACCC AGACCAGGTC CCCTACATCG TGACCTGGGA AGCCTTGGCT

 1201 TTTGACCCCC CTCCCTGGGT CAAGCCCTTT GTACACCCTA AGCCTCCGCC TCCTCTTCTT

 1261 CCATCCGCGC CGTCTCTCCC CCTTGAACCT CCTCTTTCGA CCCCGCCTCA ATCCTCCCTT

 1321 TATCCAGCCC TCACTCCTTC TCTAGGCGCC GGCCGGATCC CAGTGTGGTG GTACGTAGGA

 1381 ATTCGCCAGC ACAGTGGTCG ACCCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC

 1441 CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT

 1501 GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT

 1561 CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG

 1621 CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT

 1681 CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA

 1741 AAAAGCTTAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC GACGTCGCCG

 1801 GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG GAGGACGACT

 1861 TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG GACCAGGTGG

 1921 TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG TACGCCGAGT

 1981 GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG ACCGAGATCG

 2041 GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC TGCGTGCACT

 2101 TCGTGGCCGA GGAGCAGGAC TGAATCGATA AAATAAAAGA TTTTATTTAG TCTCCAGAAA

 2161 AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT

 2221 TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAGAAGT TCAGATCAAG GTCAGGAACA

 2281 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC

 2341 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC

 2401 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG

 2461 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT

 2521 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC

 2581 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGGGCG CCAGTCCTCC GATTGACTGA

 2641 GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT

 2701 CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA

 2761 TTTCCGACTT GTGGTCTCGC TGCCTTGGGA GGGTCTCCTC TGAGTGATTG ACTACCCGTC

 2821 AGCGGGGGTC TTCACATGCA GCATGTATCA AAATTAATTT GGTTTTTTTT CTTAAGTATT

 2881 TACATTAAAT GGCCATAGTT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG

 2941 CGTATTGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC

 3001 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA

 3061 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG

 3121 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT

 3181 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA

 3241 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC

 3301 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT

 3361 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG

 3421 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG

 3481 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT

 3541 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC

 3601 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG

 3661 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC

 3721 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT

 3781 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA

 3841 AATGAAGTTT GCGGCCGCAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT

 3901 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA

 3961 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC

 4021 AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC

 4081 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG

 4141 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC

 4201 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC

 4261 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG

 4321 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC

 4381 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT

 4441 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC

 4501 TCTTGCCCGG CGTCAACACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC

 4561 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC

 4621 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC

 4681 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA

 4741 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT

 4801 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT

 4861 CCGCGCACAT TTCCCCGAAA AGTGCCAC

//

 

pBABE-zeo其他相关逆病毒载体:

pVSV-G

pBABE

pBABE-hygro-hTERT

pBABE-neo-SV40LT

pMKO.1-GFP

pBABE-neo

pBABE-GFP

pVPack-10A1

pBABE-Puro

pCMV-Gag-Pol

pVPack-Eco

pCMV-VSV-G

pCL-Eco

pCMV-VSV-G

pMSCV-PIG

pFB-ERV

pFB-hrGFP

pFB-Neo

pCFB

pRetroX-Tight-Pur-Luc

pRetroX-TetOne

pRetroX-PTuner2

pRetroX-PTuner-IRES

pRetroX-TRE3G-Luc

pRetroX-Tet-On     Advanced

pRetroQ-mCherry-N1

pRetroX-Tight-Pur

pRetroQ-AcGFP1-C1

pRetroQ-DsRed-Monomer-C1

RNAi-Ready     pSIREN-RetroQ-DsRed-Express

pRetroX-SG2M-Cyan

pQCXIX

pLXSN

RNAi-Ready     pSIREN-RetroQ-ZsGreen1

PSUPER.RETRO.PURO

pMSCVhyg

PRETROX-IRES-ZSGREEN1

pSuper.Retro.Neo

pMSCVpuro

pMKO.1-puro

pMSCV-FLIP-puro-dsRed-GFP-miRNA

pBABE-HAII

pBABE-puro-IRES-EGFP

pBABE-Puro     SV40LT

pBABE-neo-hTERT

pBABE-zeo

pBABE-hygro

pVPack-VSV-G

pVPack-GP

pCL-Ampho

pVPack-Ampho

pCMV-VSVG

pUMVC

pCFB-EGSH

pQCXIN

pFB

pFB-Luc

pRetroX-TetOne-luc

pRetro-Lib

pRetroX-PTuner2-C

pRetroX-PTuner

pRetroX-Tet3G

pRetroX-TRE3G

pRetroX-Tight-Hyg-Luc

pRetroX-Tet-Off     Advanced

pRetroQ-mCherry-C1

pRetroX-G1-Red

pRetroQ-AcGFP1-N1

pRetroQ-DsRed-Monomer-N1

pLXIN

pLNCX2

pSuper.Retro.Neo+GFP

pSilencer5.1-U6     Retro

pRetroX-IRES-DsRedExpress

RNAi-Ready     pSIREN-RetroQ

pMSCVneo

pQCXIH

pSilencer5.1-H1     Retro

pCLXSN